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Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge

文献类型：期刊论文

作者Cong Ming1,2; Lu Jiasen3; Wu Huifeng1,2; Zhao Jianmin1,2
刊名CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
出版日期2013-09-01
卷号31期号:5页码:1002-1009
关键词cadmium Listonella anguillarum Crassostrea gigas defensive enzyme gene expression
ISSN号0254-4059
产权排序[Cong Ming; Wu Huifeng; Zhao Jianmin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Cong Ming; Wu Huifeng; Zhao Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Lu Jiasen] Yantai Univ, Sch Biol, Yantai 264005, Peoples R China
通讯作者Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
中文摘要Heavy metal pollution can affect the immune capability of organisms. We evaluated the effect of cadmium (Cd) on the defense responses of the Pacific oyster Crassostrea gigas to Listonella anguillarum challenge. The activities of several important defensive enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GPx), acid phosphatase (ACP), Na+, K+ -ATPase in gills and hepatopancreas, and phenoloxidase-like (POL) enzyme in hemolymph were assayed. In addition, the expression levels of several genes, including heat shock protein 90 (HSP90), metallothionein (MT), and bactericidal/permeability increasing (BPI) protein were quantified by fluorescent quantitative PCR. The enzyme activities of SOD, ACP, POL, and GPx in hepatopancreas, and the expression of HSP90 were down-regulated, whereas GPx activity in the gill, Na+, K+-ATPase activities in both tissues, and MT expression was increased in Cdexposed oysters post L. anguillarum challenge. However, BPI expression was not significantly altered by co-stress of L. anguillarum infection and cadmium exposure. Our results suggest that cadmium exposure alters the oysters' immune responses and energy metabolism following vibrio infection.
英文摘要Heavy metal pollution can affect the immune capability of organisms. We evaluated the effect of cadmium (Cd) on the defense responses of the Pacific oyster Crassostrea gigas to Listonella anguillarum challenge. The activities of several important defensive enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GPx), acid phosphatase (ACP), Na+, K+ -ATPase in gills and hepatopancreas, and phenoloxidase-like (POL) enzyme in hemolymph were assayed. In addition, the expression levels of several genes, including heat shock protein 90 (HSP90), metallothionein (MT), and bactericidal/permeability increasing (BPI) protein were quantified by fluorescent quantitative PCR. The enzyme activities of SOD, ACP, POL, and GPx in hepatopancreas, and the expression of HSP90 were down-regulated, whereas GPx activity in the gill, Na+, K+-ATPase activities in both tissues, and MT expression was increased in Cdexposed oysters post L. anguillarum challenge. However, BPI expression was not significantly altered by co-stress of L. anguillarum infection and cadmium exposure. Our results suggest that cadmium exposure alters the oysters' immune responses and energy metabolism following vibrio infection.
学科主题Limnology ; Oceanography
研究领域[WOS]Marine & Freshwater Biology ; Oceanography
关键词[WOS]MESSENGER-RNA ; FREE-RADICALS ; EXPOSURE ; EXPRESSION ; IMMUNE ; HEMOCYTES ; VIRGINICA ; STRESS ; GENE ; METABOLISM
收录类别SCI
资助信息100 Talents Program of Chinese Academy of Sciences; Development Plan of Science and Technology in Shandong Province [2012GGA06032]; Key Deployment Program of Chinese Academy of Sciences [KZZD-EW-14-03]
原文出处http://dx.doi.org/10.1007/s00343-013-2280-2
语种英语
WOS记录号WOS:000324111300008
公开日期2014-07-08
源URL[http://ir.yic.ac.cn/handle/133337/6953]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
3.Yantai Univ, Sch Biol, Yantai 264005, Peoples R China
推荐引用方式
GB/T 7714		 Cong Ming,Lu Jiasen,Wu Huifeng,et al. Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(5):1002-1009.
APA Cong Ming,Lu Jiasen,Wu Huifeng,&Zhao Jianmin.(2013).Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(5),1002-1009.
MLA Cong Ming,et al."Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.5(2013):1002-1009.

入库方式： OAI收割

来源：烟台海岸带研究所

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