Metabolic responses of HeLa cells to silica nanoparticles by NMR-based metabolomic analyses
文献类型：期刊论文
| 作者 | Feng, Jianghua1; Li, Jinquan1; Wu, Huifeng2 ; Chen, Zhong1
|
| 刊名 | METABOLOMICS
 |
| 出版日期 | 2013-08-01 |
| 卷号 | 9期号:4页码:874-886 |
| 关键词 | Silica nanoparticles Metabolomic analysis NMR spectroscopy Nanomedicine HeLa cell |
| ISSN号 | 1573-3882 |
| 产权排序 | [Feng, Jianghua; Li, Jinquan; Chen, Zhong] Xiamen Univ, Dept Elect Sci, Fujian Prov Key Lab Plasma & Magnet Resonance, Xiamen 361005, Peoples R China; [Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China |
| 通讯作者 | Feng, JH (reprint author), Xiamen Univ, Dept Elect Sci, Fujian Prov Key Lab Plasma & Magnet Resonance, Xiamen 361005, Peoples R China. jianghua.feng@xmu.edu.cn |
| 中文摘要 | Silica nanoparticles are increasingly used in the biomedical fields due to their excellent solubility, high stability and favorable biocompatibility. However, despite being considered of low genotoxicity, their bio-related adverse effects have attracted particular concern from both the scientific field and the public. In this study, human cervical adenocarcinoma cells (HeLa line) were exposed to 0.01 or 1.0 mg/mL of hydrophilic silica nanoparticles. The H-1 NMR spectroscopy coupled with multivariate statistical analysis were used to characterize the metabolic variations of intracellular metabolites and the compositional changes of the corresponding culture media. At the early stage of silica nanoparticles-exposure, no obvious dose-effect of HeLa cell metabolome was observed, which implied that cellular stress-response regulated the metabolic variations of HeLa cell. Silica nanoparticles induced the increases of lipids including triglyceride, LDL, VLDL and lactate/alanine ratio and the decreases of alanine, ATP, choline, creatine, glycine, glycerol, isoleucine, leucine, phenylalanine, tyrosine, and valine, which involved in membrane modification, catabolism of carbohydrate and protein, and stress-response. Subsequently, a complicated synergistic effect of stress-response and toxicological-effect dominated the biochemical process and metabolic response, which was demonstrated in the reverse changes of some metabolites including acetate, ADP, ATP, choline, creatine, glutamine, glycine, lysine, methionine, phenylalanine and valine between 6 and 48 h post-treatment of silica nanoparticles. The toxicological-effects induced by high-dosage silica nanoparticles could be derived from the elevated levels of ATP and ADP, the utilization of glucose and amino acids and the production of metabolic end-products such as glutamate, glycine, lysine, methionine, phenylalanine, and valine. The results indicated that it is important and necessary to pursue further the physiological responses of silica nanoparticles in animal models and human before their practical use. NMR-based metabolomic analysis helps to understand the biological mechanisms of silica nanoparticles and their metabolic fate, and further, it offers an ideal platform for establishing the bio-safety of existing and new nanomaterials. |
| 英文摘要 | Silica nanoparticles are increasingly used in the biomedical fields due to their excellent solubility, high stability and favorable biocompatibility. However, despite being considered of low genotoxicity, their bio-related adverse effects have attracted particular concern from both the scientific field and the public. In this study, human cervical adenocarcinoma cells (HeLa line) were exposed to 0.01 or 1.0 mg/mL of hydrophilic silica nanoparticles. The H-1 NMR spectroscopy coupled with multivariate statistical analysis were used to characterize the metabolic variations of intracellular metabolites and the compositional changes of the corresponding culture media. At the early stage of silica nanoparticles-exposure, no obvious dose-effect of HeLa cell metabolome was observed, which implied that cellular stress-response regulated the metabolic variations of HeLa cell. Silica nanoparticles induced the increases of lipids including triglyceride, LDL, VLDL and lactate/alanine ratio and the decreases of alanine, ATP, choline, creatine, glycine, glycerol, isoleucine, leucine, phenylalanine, tyrosine, and valine, which involved in membrane modification, catabolism of carbohydrate and protein, and stress-response. Subsequently, a complicated synergistic effect of stress-response and toxicological-effect dominated the biochemical process and metabolic response, which was demonstrated in the reverse changes of some metabolites including acetate, ADP, ATP, choline, creatine, glutamine, glycine, lysine, methionine, phenylalanine and valine between 6 and 48 h post-treatment of silica nanoparticles. The toxicological-effects induced by high-dosage silica nanoparticles could be derived from the elevated levels of ATP and ADP, the utilization of glucose and amino acids and the production of metabolic end-products such as glutamate, glycine, lysine, methionine, phenylalanine, and valine. The results indicated that it is important and necessary to pursue further the physiological responses of silica nanoparticles in animal models and human before their practical use. NMR-based metabolomic analysis helps to understand the biological mechanisms of silica nanoparticles and their metabolic fate, and further, it offers an ideal platform for establishing the bio-safety of existing and new nanomaterials. |
| 学科主题 | Endocrinology & Metabolism |
| 研究领域[WOS] | Endocrinology & Metabolism |
| 关键词[WOS] | SPINNING H-1-NMR SPECTROSCOPY ; IN-VITRO ; DIOXIDE ; DELIVERY ; SIO2 ; MITOCHONDRIAL ; CONSEQUENCES ; CHEMOMETRICS ; EXPRESSION ; PULMONARY |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [20605025, 21175099, 81272581]; Fundamental Research Funds for the Central Universities [2011121046] |
| 原文出处 | http://dx.doi.org/10.1007/s11306-013-0499-8 |
| 语种 | 英语 |
| WOS记录号 | WOS:000322019100013 |
| 公开日期 | 2014-07-08 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/6967]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Xiamen Univ, Dept Elect Sci, Fujian Prov Key Lab Plasma & Magnet Resonance, Xiamen 361005, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Jianghua,Li, Jinquan,Wu, Huifeng,et al. Metabolic responses of HeLa cells to silica nanoparticles by NMR-based metabolomic analyses[J]. METABOLOMICS,2013,9(4):874-886. |
| APA | Feng, Jianghua,Li, Jinquan,Wu, Huifeng,&Chen, Zhong.(2013).Metabolic responses of HeLa cells to silica nanoparticles by NMR-based metabolomic analyses.METABOLOMICS,9(4),874-886. |
| MLA | Feng, Jianghua,et al."Metabolic responses of HeLa cells to silica nanoparticles by NMR-based metabolomic analyses".METABOLOMICS 9.4(2013):874-886. |
入库方式： OAI收割
来源：烟台海岸带研究所
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