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Tetra(p-tolyl)borate-Functionalized Solvent Polymeric Membrane: A Facile and Sensitive Sensing Platform for Peroxidase and Peroxidase Mimetics

文献类型：期刊论文

作者Wang, Xuewei1,2; Qin, Wei1
刊名CHEMISTRY-A EUROPEAN JOURNAL
出版日期2013-07-22
卷号19期号:30页码:9979-9986
关键词biosensors intermediate-sensitive membranes peroxidase peroxidase mimetics potentiometry
ISSN号0947-6539
产权排序[Wang, Xuewei; Qin, Wei] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Wang, Xuewei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者Qin, W (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn
中文摘要The determination of peroxidase activities is the basis for enzyme-labeled bioaffinity assays, peroxidase-mimicking DNAzymes- and nanoparticles-based assays, and characterization of the catalytic functions of peroxidase mimetics. Here, a facile, sensitive, and cost-effective solvent polymeric membrane-based peroxidase detection platform is described that utilizes reaction intermediates with different pK(a) values from those of substrates and final products. Several key but long-debated intermediates in the peroxidative oxidation of o-phenylenediamine (o-PD) have been identified and their charge states have been estimated. By using a solvent polymeric membrane functionalized by an appropriate substituted tetraphenylborate as a receptor, those cationic intermediates could be transferred into the membrane from the aqueous phase to induce a large cationic potential response. Thus, the potentiometric indication of the o-PD oxidation catalyzed by peroxidase or its mimetics can be fulfilled. Horseradish peroxidase has been detected with a detection limit at least two orders of magnitude lower than those obtained by spectrophotometric techniques and traditional membrane-based methods. As an example of peroxidase mimetics, G-quadruplex DNAzymes were probed by the intermediate-sensitive membrane and a label-free thrombin detection protocol was developed based on the catalytic activity of the thrombin-binding G-quadruplex aptamer.
英文摘要The determination of peroxidase activities is the basis for enzyme-labeled bioaffinity assays, peroxidase-mimicking DNAzymes- and nanoparticles-based assays, and characterization of the catalytic functions of peroxidase mimetics. Here, a facile, sensitive, and cost-effective solvent polymeric membrane-based peroxidase detection platform is described that utilizes reaction intermediates with different pK(a) values from those of substrates and final products. Several key but long-debated intermediates in the peroxidative oxidation of o-phenylenediamine (o-PD) have been identified and their charge states have been estimated. By using a solvent polymeric membrane functionalized by an appropriate substituted tetraphenylborate as a receptor, those cationic intermediates could be transferred into the membrane from the aqueous phase to induce a large cationic potential response. Thus, the potentiometric indication of the o-PD oxidation catalyzed by peroxidase or its mimetics can be fulfilled. Horseradish peroxidase has been detected with a detection limit at least two orders of magnitude lower than those obtained by spectrophotometric techniques and traditional membrane-based methods. As an example of peroxidase mimetics, G-quadruplex DNAzymes were probed by the intermediate-sensitive membrane and a label-free thrombin detection protocol was developed based on the catalytic activity of the thrombin-binding G-quadruplex aptamer.
学科主题Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]QUADRUPLEX-BASED DNAZYME ; ION-SELECTIVE ELECTRODE ; BENZOQUINONE DI-IMINES ; HORSERADISH-PEROXIDASE ; O-PHENYLENEDIAMINE ; COLORIMETRIC DETECTION ; ELECTROCHEMICAL ASSAY ; CATALYZED OXIDATION ; ENZYME-IMMUNOASSAY ; HYDROGEN-PEROXIDE
收录类别SCI
资助信息Instrument Developing Project of the Chinese Academy of Sciences [YZ201161]; National Natural Science Foundation of China [41176081]; Taishan Scholar Program of Shandong Province [TS20081159]
原文出处http://dx.doi.org/10.1002/chem.201300284
语种英语
WOS记录号WOS:000321766400036
公开日期2014-07-08
源URL[http://ir.yic.ac.cn/handle/133337/6973]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Xuewei,Qin, Wei. Tetra(p-tolyl)borate-Functionalized Solvent Polymeric Membrane: A Facile and Sensitive Sensing Platform for Peroxidase and Peroxidase Mimetics[J]. CHEMISTRY-A EUROPEAN JOURNAL,2013,19(30):9979-9986.
APA Wang, Xuewei,&Qin, Wei.(2013).Tetra(p-tolyl)borate-Functionalized Solvent Polymeric Membrane: A Facile and Sensitive Sensing Platform for Peroxidase and Peroxidase Mimetics.CHEMISTRY-A EUROPEAN JOURNAL,19(30),9979-9986.
MLA Wang, Xuewei,et al."Tetra(p-tolyl)borate-Functionalized Solvent Polymeric Membrane: A Facile and Sensitive Sensing Platform for Peroxidase and Peroxidase Mimetics".CHEMISTRY-A EUROPEAN JOURNAL 19.30(2013):9979-9986.

入库方式： OAI收割

来源：烟台海岸带研究所

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