A Novel C-Type Lysozyme from Mytilus galloprovincialis: Insight into Innate Immunity and Molecular Evolution of Invertebrate C-Type Lysozymes
文献类型：期刊论文
| 作者 | Wang, Qing1 ; Wang, Chunyan2; Mu, Changkao2; Wu, Huifeng1; Zhang, Linbao1; Zhao, Jianmin1
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| 刊名 | PLOS ONE
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| 出版日期 | 2013-06-20 |
| 卷号 | 8期号:6页码:e67469 |
| 关键词 | EGG-WHITE LYSOZYME EXPRESSION ANALYSIS DROSOPHILA-MELANOGASTER MAXIMUM-LIKELIHOOD POSITIVE SELECTION SHRIMP LYSOZYME GENE-EXPRESSION SALIVARY-GLANDS LYTIC ACTIVITY BOMBYX-MORI |
| ISSN号 | 1932-6203 |
| 产权排序 | [Wang, Qing; Wu, Huifeng; Zhang, Linbao; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai, Peoples R China; [Wang, Chunyan; Mu, Changkao] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China |
| 通讯作者 | Wu, HF (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai, Peoples R China. hfwu@yic.ac.cn ; jmzhao@yic.ac.cn |
| 中文摘要 | A c-type lysozyme (named as MgCLYZ) gene was cloned from the mussel Mytilus galloprovincialis. Blast analysis indicated that MgCLYZ was a salivary c-type lysozyme which was mainly found in insects. The nucleotide sequence of MgCLYZ was predicted to encode a polypeptide of 154 amino acid residues with the signal peptide comprising the first 24 residues. The deduced mature peptide of MgCLYZ was of a calculated molecular weight of 14.4 kD and a theoretical isoelectric point (pI) of 8.08. Evolution analysis suggested that bivalve branch of the invertebrate c-type lysozymes phylogeny tree underwent positive selection during evolution. By quantitative real-time RT-PCR (qRT-PCR) analysis, MgCLYZ transcript was widely detected in all examined tissues and responded sensitively to bacterial challenge in hemocytes and hepatopancreas. The optimal temperature and pH of recombinant MgCLYZ (rMgCLYZ) were 20 degrees C and 4, respectively. The rMgCLYZ displayed lytic activities against Gram-positive bacteria including Micrococcus luteus and Staphyloccocus aureus, and Gram-negative bacteria including Vibrio anguillarum, Enterobacter cloacae, Pseudomonas putida, Proteus mirabilis and Bacillus aquimaris. These results suggest that MgCLYZ perhaps play an important role in innate immunity of M. galloprovincialis, and invertebrate c-type lysozymes might be under positive selection in a species-specific manner during evolution for undergoing adaptation to different environment and diverse pathogens. |
| 英文摘要 | A c-type lysozyme (named as MgCLYZ) gene was cloned from the mussel Mytilus galloprovincialis. Blast analysis indicated that MgCLYZ was a salivary c-type lysozyme which was mainly found in insects. The nucleotide sequence of MgCLYZ was predicted to encode a polypeptide of 154 amino acid residues with the signal peptide comprising the first 24 residues. The deduced mature peptide of MgCLYZ was of a calculated molecular weight of 14.4 kD and a theoretical isoelectric point (pI) of 8.08. Evolution analysis suggested that bivalve branch of the invertebrate c-type lysozymes phylogeny tree underwent positive selection during evolution. By quantitative real-time RT-PCR (qRT-PCR) analysis, MgCLYZ transcript was widely detected in all examined tissues and responded sensitively to bacterial challenge in hemocytes and hepatopancreas. The optimal temperature and pH of recombinant MgCLYZ (rMgCLYZ) were 20 degrees C and 4, respectively. The rMgCLYZ displayed lytic activities against Gram-positive bacteria including Micrococcus luteus and Staphyloccocus aureus, and Gram-negative bacteria including Vibrio anguillarum, Enterobacter cloacae, Pseudomonas putida, Proteus mirabilis and Bacillus aquimaris. These results suggest that MgCLYZ perhaps play an important role in innate immunity of M. galloprovincialis, and invertebrate c-type lysozymes might be under positive selection in a species-specific manner during evolution for undergoing adaptation to different environment and diverse pathogens. |
| 学科主题 | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | EGG-WHITE LYSOZYME ; EXPRESSION ANALYSIS ; DROSOPHILA-MELANOGASTER ; MAXIMUM-LIKELIHOOD ; POSITIVE SELECTION ; SHRIMP LYSOZYME ; GENE-EXPRESSION ; SALIVARY-GLANDS ; LYTIC ACTIVITY ; BOMBYX-MORI |
| 收录类别 | SCI |
| 资助信息 | NSFC [31172388]; 100 Talents Program of the Chinese Academy of Sciences; Department of Science and Technology of Yantai City [2011067] |
| 原文出处 | http://dx.doi.org/10.1371/journal.pone.0067469 |
| 语种 | 英语 |
| WOS记录号 | WOS:000322342800136 |
| 公开日期 | 2014-07-08 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/6983]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai, Peoples R China 2.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Qing,Wang, Chunyan,Mu, Changkao,et al. A Novel C-Type Lysozyme from Mytilus galloprovincialis: Insight into Innate Immunity and Molecular Evolution of Invertebrate C-Type Lysozymes[J]. PLOS ONE,2013,8(6):e67469. |
| APA | Wang, Qing,Wang, Chunyan,Mu, Changkao,Wu, Huifeng,Zhang, Linbao,&Zhao, Jianmin.(2013).A Novel C-Type Lysozyme from Mytilus galloprovincialis: Insight into Innate Immunity and Molecular Evolution of Invertebrate C-Type Lysozymes.PLOS ONE,8(6),e67469. |
| MLA | Wang, Qing,et al."A Novel C-Type Lysozyme from Mytilus galloprovincialis: Insight into Innate Immunity and Molecular Evolution of Invertebrate C-Type Lysozymes".PLOS ONE 8.6(2013):e67469. |
入库方式： OAI收割
来源：烟台海岸带研究所
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