Construction of a full-length cDNA library of Solen grandis Dunker and identification of defense- and immune-related genes
文献类型：期刊论文
| 作者 | Sun Guohua1; Liu Xiangquan1; Ren Lihua1; Yang Jianmin1; Wei Xiumei1; Yang Jialong2 |
| 刊名 | JOURNAL OF OCEAN UNIVERSITY OF CHINA
 |
| 出版日期 | 2014-02-01 |
| 卷号 | 13期号:1页码:169-173 |
| 关键词 | Solen grandis cDNA library EST sequence defense and immune functional gene |
| ISSN号 | 1672-5182 |
| 产权排序 | [Sun Guohua; Liu Xiangquan; Ren Lihua; Yang Jianmin; Wei Xiumei] Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China; [Yang Jialong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 通讯作者 | Liu, XQ (reprint author), Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China. lxq6808@163.com |
| 中文摘要 | The basic genetic characteristics, important functional genes, and entire transcriptome of Solen grandis Dunker were investigated by constructing a full-length cDNA library with the 'switching mechanism at the 5'-end of the RNA transcript' (SMART) technique. Total RNA was isolated from the immune-relevant tissues, gills and hemocytes, using the Trizol reagent, and cDNA fragments were digested with Sfi I before being ligated to the pBluescript II SK* vector. The cDNA library had a titer of 1048 cfu mu L-1 and a storage capacity of 1.05x10(6) cfu. Approximately 98% of the clones in the library were recombinants, and the fragment lengths of insert cDNA ranged from 0.8 kb to 3.0 kb. A total of 2038 expressed sequence tags were successfully sequenced and clustered into 965 unigenes. BLASTN analysis showed that 240 sequences were highly similar to the known genes (E-value < 1e (-5); percent identity > 80%), accounting for 25% of the total unigenes. According to the Gene Ontology, these unigenes were related to several biological processes, including cell structure, signal transport, protein synthesis, transcription, energy metabolism, and immunity. Fifteen of the identified sequences were related to defense and immunity. The full-length cDNA sequence of HSC70 was obtained. The cDNA library of S. grandis provided a useful resource for future researches of functional genomics related to stress tolerance, immunity, and other physiological activities. |
| 英文摘要 | The basic genetic characteristics, important functional genes, and entire transcriptome of Solen grandis Dunker were investigated by constructing a full-length cDNA library with the 'switching mechanism at the 5'-end of the RNA transcript' (SMART) technique. Total RNA was isolated from the immune-relevant tissues, gills and hemocytes, using the Trizol reagent, and cDNA fragments were digested with Sfi I before being ligated to the pBluescript II SK* vector. The cDNA library had a titer of 1048 cfu mu L-1 and a storage capacity of 1.05x10(6) cfu. Approximately 98% of the clones in the library were recombinants, and the fragment lengths of insert cDNA ranged from 0.8 kb to 3.0 kb. A total of 2038 expressed sequence tags were successfully sequenced and clustered into 965 unigenes. BLASTN analysis showed that 240 sequences were highly similar to the known genes (E-value < 1e (-5); percent identity > 80%), accounting for 25% of the total unigenes. According to the Gene Ontology, these unigenes were related to several biological processes, including cell structure, signal transport, protein synthesis, transcription, energy metabolism, and immunity. Fifteen of the identified sequences were related to defense and immunity. The full-length cDNA sequence of HSC70 was obtained. The cDNA library of S. grandis provided a useful resource for future researches of functional genomics related to stress tolerance, immunity, and other physiological activities. |
| 学科主题 | Oceanography |
| 研究领域[WOS] | Oceanography |
| 关键词[WOS] | OYSTER CRASSOSTREA-GIGAS ; HEAT-SHOCK PROTEINS ; PACIFIC OYSTER ; HSP70 GENES ; EXPRESSION ; HEAT-SHOCK-PROTEIN-70 ; EVOLUTION ; APOPTOSIS ; DISEASE ; FAMILY |
| 收录类别 | SCI |
| 资助信息 | Shandong Program of Agriculture Thoroughbred Project; Marine Special Research Foundation of China [200805031]; Taishan Scholar position funding of Aquatic Animal Nutrition and Feed; NSFC [31202025] |
| 原文出处 | http://dx.doi.org/10.1007/s11802-014-2025-7 |
| 语种 | 英语 |
| WOS记录号 | WOS:000330312000022 |
| 公开日期 | 2014-07-08 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/7059]  |
| 专题 | 烟台海岸带研究所_中科院烟台海岸带研究所知识产出 |
| 作者单位 | 1.Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun Guohua,Liu Xiangquan,Ren Lihua,et al. Construction of a full-length cDNA library of Solen grandis Dunker and identification of defense- and immune-related genes[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2014,13(1):169-173. |
| APA | Sun Guohua,Liu Xiangquan,Ren Lihua,Yang Jianmin,Wei Xiumei,&Yang Jialong.(2014).Construction of a full-length cDNA library of Solen grandis Dunker and identification of defense- and immune-related genes.JOURNAL OF OCEAN UNIVERSITY OF CHINA,13(1),169-173. |
| MLA | Sun Guohua,et al."Construction of a full-length cDNA library of Solen grandis Dunker and identification of defense- and immune-related genes".JOURNAL OF OCEAN UNIVERSITY OF CHINA 13.1(2014):169-173. |
入库方式： OAI收割
来源：烟台海岸带研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。