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Molecular Cloning, Sequence Analysis and Phylogeny of First Caudata G-type Lysozyme in Axolotl (Ambystoma mexicanum)

文献类型：期刊论文

作者Yu, Haining1,2; Gao, Jiuxiang1; Lu, Yiling1; Guang, Huijuan1; Cai, Shasha1; Zhang, Songyan1; Wang, Yipeng3
刊名ZOOLOGICAL SCIENCE
出版日期2013-11-01
卷号30期号:11页码:938-943
关键词Ambystoma mexicanum caudate amphibian lysozyme molecular cloning phylogeny
ISSN号0289-0003
其他题名Molecularly imprinted nanoparticles based potentiometric sensor with a nanomolar detection limit.pdf
产权排序[Yu, Haining; Gao, Jiuxiang; Lu, Yiling; Guang, Huijuan; Cai, Shasha; Zhang, Songyan] Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China; [Yu, Haining] Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China; [Wang, Yipeng] Chinese Acad Sci, Yantai Inst Coastal Zone Reseach, Yantai 264003, Shandong, Peoples R China
通讯作者Yu, HN (reprint author), Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China. joannyu@live.cn
中文摘要Lysozymes are key proteins that play important roles in innate immune defense in many animal phyla by breaking down the bacterial cell-walls. In this study, we report the molecular cloning, sequence analysis and phylogeny of the first caudate amphibian g-lysozyme: a full-length spleen cDNA library from axolotl (Ambystoma mexicanum). A goose-type (g-lysozyme) EST was identified and the full-length cDNA was obtained using RACE-PCR. The axolotl g-lysozyme sequence represents an open reading frame for a putative signal peptide and the mature protein composed of 184 amino acids. The calculated molecular mass and the theoretical isoelectric point (pI) of this mature protein are 21523.0 Da and 4.37, respectively. Expression of g-lysozyme mRNA is predominantly found in skin, with lower levels in spleen, liver, muscle, and lung. Phylogenetic analysis revealed that caudate amphibian g-lysozyme had distinct evolution pattern for being juxtaposed with not only anura amphibian, but also with the fish, bird and mammal. Although the first complete cDNA sequence for caudate amphibian g-lysozyme is reported in the present study, clones encoding axolotl's other functional immune molecules in the full-length cDNA library will have to be further sequenced to gain insight into the fundamental aspects of antibacterial mechanisms in caudate.
英文摘要Lysozymes are key proteins that play important roles in innate immune defense in many animal phyla by breaking down the bacterial cell-walls. In this study, we report the molecular cloning, sequence analysis and phylogeny of the first caudate amphibian g-lysozyme: a full-length spleen cDNA library from axolotl (Ambystoma mexicanum). A goose-type (g-lysozyme) EST was identified and the full-length cDNA was obtained using RACE-PCR. The axolotl g-lysozyme sequence represents an open reading frame for a putative signal peptide and the mature protein composed of 184 amino acids. The calculated molecular mass and the theoretical isoelectric point (pI) of this mature protein are 21523.0 Da and 4.37, respectively. Expression of g-lysozyme mRNA is predominantly found in skin, with lower levels in spleen, liver, muscle, and lung. Phylogenetic analysis revealed that caudate amphibian g-lysozyme had distinct evolution pattern for being juxtaposed with not only anura amphibian, but also with the fish, bird and mammal. Although the first complete cDNA sequence for caudate amphibian g-lysozyme is reported in the present study, clones encoding axolotl's other functional immune molecules in the full-length cDNA library will have to be further sequenced to gain insight into the fundamental aspects of antibacterial mechanisms in caudate.
学科主题Zoology
研究领域[WOS]Zoology
关键词[WOS]GOOSE-TYPE LYSOZYME ; TAPES-JAPONICA LYSOZYME ; C-TYPE ; RECOMBINANT PROTEIN ; ALPHA-LACTALBUMIN ; LYTIC ACTIVITY ; EXPRESSION ; EVOLUTION ; CDNA ; GENE
收录类别SCI
资助信息Chinese National Natural Science Foundation [41076098, 41206153]
原文出处http://dx.doi.org/10.2108/zsj.30.938
语种英语
WOS记录号WOS:000326246300008
公开日期2014-07-08
源URL[http://ir.yic.ac.cn/handle/133337/7062]  
专题烟台海岸带研究所_中科院烟台海岸带研究所知识产出
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China
2.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Reseach, Yantai 264003, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Yu, Haining,Gao, Jiuxiang,Lu, Yiling,et al. Molecular Cloning, Sequence Analysis and Phylogeny of First Caudata G-type Lysozyme in Axolotl (Ambystoma mexicanum)[J]. ZOOLOGICAL SCIENCE,2013,30(11):938-943.
APA Yu, Haining.,Gao, Jiuxiang.,Lu, Yiling.,Guang, Huijuan.,Cai, Shasha.,...&Wang, Yipeng.(2013).Molecular Cloning, Sequence Analysis and Phylogeny of First Caudata G-type Lysozyme in Axolotl (Ambystoma mexicanum).ZOOLOGICAL SCIENCE,30(11),938-943.
MLA Yu, Haining,et al."Molecular Cloning, Sequence Analysis and Phylogeny of First Caudata G-type Lysozyme in Axolotl (Ambystoma mexicanum)".ZOOLOGICAL SCIENCE 30.11(2013):938-943.

入库方式： OAI收割

来源：烟台海岸带研究所

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