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Identification of a LPS-induced TNF-alpha factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation

文献类型：期刊论文

作者Yang, Dinglong1,2; Wei, Xiumei1; Yang, Jianmin1; Yang, Jialong3; Xu, Jie1,2; Fang, Jinghui1; Wang, Sheng1,2; Liu, Xiangquan1
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2013-10-01
卷号35期号:4页码:1325-1328
关键词Solen grandis LITAF Innate immunity Transcription factor Real-time PCR
ISSN号1050-4648
产权排序[Yang, Dinglong; Wei, Xiumei; Yang, Jianmin; Xu, Jie; Fang, Jinghui; Wang, Sheng; Liu, Xiangquan] Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China; [Yang, Dinglong; Xu, Jie; Wang, Sheng] Shanghai Ocean Univ, Sch Fisheries & Life Sci, Shanghai 201306, Peoples R China; [Yang, Jialong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
通讯作者Wei, XM (reprint author), Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China. xiumeiwei@hotmail.com ; lxq6808@163.com
中文摘要Lipopolysaccharide-induced TNF-alpha factor (LITAF) is one of the most important transcription factors mediating TNF-alpha transcription. In the present study, a LITAF gene (designated as SgLITAF) was identified from razor clams Solen grandis. The full-length cDNA of SgLITAF was of 1476 bp, encoding a polypeptide of 130 amino acids showed high similarity to other known LITAFs. SgLITAF encoded a LITAF domain and the Zn2+-binding motifs in the domain were well conserved. The mRNA transcripts of SgLITAF were detected in all tested tissues of healthy razor clams, including mantle, gill, gonad, hemocytes, muscle and hepatopancreas, and with the highest expression level in hepatopancreas. The expression level of SgLITAF in hemocytes was significantly up-regulated (P < 0.01) after razor clams were stimulated by LPS or beta-1, 3-glucan, but no obvious fluctuation of SgLITAF mRNA expression was observed after PGN stimulation. All the results indicated that there might be a LITAF-regulated TNF-alpha signaling pathway existing in S. grandis, which involved in the immune response not only against gram-negative bacteria but also towards fungi. (C) 2013 Elsevier Ltd. All rights reserved.
英文摘要Lipopolysaccharide-induced TNF-alpha factor (LITAF) is one of the most important transcription factors mediating TNF-alpha transcription. In the present study, a LITAF gene (designated as SgLITAF) was identified from razor clams Solen grandis. The full-length cDNA of SgLITAF was of 1476 bp, encoding a polypeptide of 130 amino acids showed high similarity to other known LITAFs. SgLITAF encoded a LITAF domain and the Zn2+-binding motifs in the domain were well conserved. The mRNA transcripts of SgLITAF were detected in all tested tissues of healthy razor clams, including mantle, gill, gonad, hemocytes, muscle and hepatopancreas, and with the highest expression level in hepatopancreas. The expression level of SgLITAF in hemocytes was significantly up-regulated (P < 0.01) after razor clams were stimulated by LPS or beta-1, 3-glucan, but no obvious fluctuation of SgLITAF mRNA expression was observed after PGN stimulation. All the results indicated that there might be a LITAF-regulated TNF-alpha signaling pathway existing in S. grandis, which involved in the immune response not only against gram-negative bacteria but also towards fungi. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]MOLECULAR-CLONING ; HOMOLOG ; GENE
收录类别SCI
资助信息NSFC [31202025]; Program of Agriculture Thoroughbred Project, Shandong Province, China; Taishan Scholar Position Funding of Aquatic Animal Nutrition and Feed
原文出处http://dx.doi.org/10.1016/j.fsi.2013.07.034
语种英语
WOS记录号WOS:000324849800031
公开日期2014-07-08
源URL[http://ir.yic.ac.cn/handle/133337/7064]  
专题烟台海岸带研究所_中科院烟台海岸带研究所知识产出
海岸带生物资源高效利用研究与发展中心
作者单位1.Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China
2.Shanghai Ocean Univ, Sch Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Dinglong,Wei, Xiumei,Yang, Jianmin,et al. Identification of a LPS-induced TNF-alpha factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(4):1325-1328.
APA Yang, Dinglong.,Wei, Xiumei.,Yang, Jianmin.,Yang, Jialong.,Xu, Jie.,...&Liu, Xiangquan.(2013).Identification of a LPS-induced TNF-alpha factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation.FISH & SHELLFISH IMMUNOLOGY,35(4),1325-1328.
MLA Yang, Dinglong,et al."Identification of a LPS-induced TNF-alpha factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation".FISH & SHELLFISH IMMUNOLOGY 35.4(2013):1325-1328.

入库方式： OAI收割

来源：烟台海岸带研究所

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