A Highly Efficient Synthetic Vector: Nonhydrodynamic Delivery of DNA to Hepatocyte Nuclei in Vivo
文献类型：期刊论文
| 作者 | Hu, Yunxia
|
| 刊名 | ACS NANO
 |
| 出版日期 | 2013-06 |
| 卷号 | 7期号:6页码:5376-5384 |
| 关键词 | nanoparticle liposome liver hepatocyte DNA delivery arginine-rich peptide |
| ISSN号 | 1936-0851 |
| 产权排序 | [Hu, Yunxia; Haynes, Matthew T.; Wang, Yuhua; Liu, Feng; Huang, Leaf] Univ N Carolina, Eshelman Sch Pharm, Div Mol Pharmaceut, Ctr Nanotechnol Drug Delivery, Chapel Hill, NC 27599 USA |
| 通讯作者 | Hu, YX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chun Hui Rd, Yantai 264003, Shandong, Peoples R China. yunxiahu@yic.ac.cn ; leafh@unc.edu |
| 中文摘要 | Multifunctional membrane-core nanoparticles, composed of calcium phosphate cores, arginine-rich peptides, cationic and PEGylated lipid membranes, and galactose targeting ligands, have been developed as synthetic vectors for efficient nuclear delivery of plasmid DNA and subsequent gene expression in hepatocytes in vivo. Targeted particles exhibited rapid and extensive hepatic accumulation and were predominantly internalized by hepatocytes, while the Inclusion of such peptides in Le was sufficient to elicit high degrees of nuclear translocation of plasmid DNA. Monocyclic CR8C significantly enhanced In vivo gene expression over 10-fold more than linear CR8C, likely due to a release-favoring mechanism of the DNA/peptide complex. Though 100-fold lower in activity than that achieved via hydrodynamic injection, this formulation presents as a much less invasive alternative. To our knowledge, this is the most effective synthetic vector for liver gene transfer. |
| 学科主题 | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 收录类别 | SCI |
| 资助信息 | NIH [CA129835, CA149363, CA125273, CA151652] |
| 原文出处 | http://dx.doi.org/10.1021/nn4012384 |
| 语种 | 英语 |
| 公开日期 | 2014-07-08 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/7058]  |
| 专题 | 烟台海岸带研究所_山东省海岸带环境工程技术研究中心 |
| 推荐引用方式 GB/T 7714 | Hu, Yunxia. A Highly Efficient Synthetic Vector: Nonhydrodynamic Delivery of DNA to Hepatocyte Nuclei in Vivo[J]. ACS NANO,2013,7(6):5376-5384. |
| APA | Hu, Yunxia.(2013).A Highly Efficient Synthetic Vector: Nonhydrodynamic Delivery of DNA to Hepatocyte Nuclei in Vivo.ACS NANO,7(6),5376-5384. |
| MLA | Hu, Yunxia."A Highly Efficient Synthetic Vector: Nonhydrodynamic Delivery of DNA to Hepatocyte Nuclei in Vivo".ACS NANO 7.6(2013):5376-5384. |
入库方式： OAI收割
来源：烟台海岸带研究所
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