A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression
文献类型：期刊论文
| 作者 | Zhang Jiquan1; Wang Jing1,2 ; Xiang Jianhai1
|
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2013-11-01 |
| 卷号 | 31期号:6页码:1204-1209 |
| 关键词 | metallothionein Crustacea Decapoda embryonic stage larvae post larvae |
| ISSN号 | 0254-4059 |
| 通讯作者 | Zhang, JQ |
| 中文摘要 | Metallothioneins (MTs) are a group of low molecular weight cysteine-rich proteins capable of binding heavy metal ions. A cadmium metallothionein (EcMT-Cd) cDNA with a 189 bp open reading frame (ORF) that encoded a 62 amino acid protein was obtained from Exopalaemon carinicauda. Seventeen cysteines were in the deduced amino acid sequence, and the cysteine (Cys)-rich characteristic was revealed in different metallothioneins in other species. In addition, the deduced amino acid sequence did not contain any aromatic amino acid residues, such as tyrosine (Tyr), tryptophan (Trp), and phenylalanine (Phe). EcMT-Cd mRNA was expressed in all tested tissues (the ovary, muscle, stomach, and hepatopancreas), and its expression profiles in the hepatopancreas were very different when shrimps were exposed to seawater containing either 50 mu mol/L CuSO4 or 2.5 mu mol/L CdCl2. The expression of EcMT-Cd was significantly up-regulated in shrimp exposed to CuSO4 for 12 h and down-regulated in shrimps exposed to CdCl2 for 12 h. After 24 h exposure to both metals, its expression was down-regulated. By contrast, at 48 h the EcMT-Cd was up-regulated in test shrimps exposed to CdCl2. The transcript of EcMT-Cd was very low or even absent before the zoea stage, and the expression of EcMT-Cd was detected from mysis larvae-I, then its expression began to rise. In conclusion, a cadmium MT exists in E. carinicauda that is expressed in different tissues and during different developmental stages, and responds to the challenge with heavy metal ions, which provides a clue to understanding the function of cadmium MT. |
| 英文摘要 | Metallothioneins (MTs) are a group of low molecular weight cysteine-rich proteins capable of binding heavy metal ions. A cadmium metallothionein (EcMT-Cd) cDNA with a 189 bp open reading frame (ORF) that encoded a 62 amino acid protein was obtained from Exopalaemon carinicauda. Seventeen cysteines were in the deduced amino acid sequence, and the cysteine (Cys)-rich characteristic was revealed in different metallothioneins in other species. In addition, the deduced amino acid sequence did not contain any aromatic amino acid residues, such as tyrosine (Tyr), tryptophan (Trp), and phenylalanine (Phe). EcMT-Cd mRNA was expressed in all tested tissues (the ovary, muscle, stomach, and hepatopancreas), and its expression profiles in the hepatopancreas were very different when shrimps were exposed to seawater containing either 50 mu mol/L CuSO4 or 2.5 mu mol/L CdCl2. The expression of EcMT-Cd was significantly up-regulated in shrimp exposed to CuSO4 for 12 h and down-regulated in shrimps exposed to CdCl2 for 12 h. After 24 h exposure to both metals, its expression was down-regulated. By contrast, at 48 h the EcMT-Cd was up-regulated in test shrimps exposed to CdCl2. The transcript of EcMT-Cd was very low or even absent before the zoea stage, and the expression of EcMT-Cd was detected from mysis larvae-I, then its expression began to rise. In conclusion, a cadmium MT exists in E. carinicauda that is expressed in different tissues and during different developmental stages, and responds to the challenge with heavy metal ions, which provides a clue to understanding the function of cadmium MT. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 学科主题 | Marine & Freshwater Biology ; Oceanography |
| 类目[WOS] | Limnology ; Oceanography |
| 研究领域[WOS] | Marine & Freshwater Biology ; Oceanography |
| 关键词[WOS] | CRAB CALLINECTES-SAPIDUS ; MT MESSENGER-RNA ; METAL ; ZINC ; EMBRYOGENESIS ; ACCUMULATION ; INDUCTION ; MERCURY ; PROTEIN ; CLONING |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s00343-013-3089-8 |
| 语种 | 英语 |
| WOS记录号 | WOS:000326628800006 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16509]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Jiquan,Wang Jing,Xiang Jianhai. A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(6):1204-1209. |
| APA | Zhang Jiquan,Wang Jing,&Xiang Jianhai.(2013).A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(6),1204-1209. |
| MLA | Zhang Jiquan,et al."A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.6(2013):1204-1209. |
入库方式： OAI收割
来源：海洋研究所
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