A four-CRD C-type lectin from Chlamys farreri mediating nonself-recognition with broader spectrum and opsonization
文献类型：期刊论文
| 作者 | Huang, Mengmeng1,2 ; Wang, Lingling1; Yang, Jialong1,2; Zhang, Huan1; Wang, Leilei1,2; Song, Linsheng1; Zhang, H
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| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 出版日期 | 2013-04-01 |
| 卷号 | 39期号:4页码:363-369 |
| ISSN号 | 0145-305X |
| 关键词 | Chlamys farreri C-type lectin Innate immunity Nonself-recognition Opsonization Microbe binding ability |
| 通讯作者 | Zhang, H |
| 中文摘要 | C-type lectins are a superfamily of Ca2+-dependent carbohydrate-recognition proteins consisting of at least one carbohydrate-recognition domain (CRD), which participate in nonself-recognition and clearance of invaders. In invertebrate, some multidomain C-type lectins have been identified, but their relative functions and binding mechanism are still meager. In the present study, A C-type lectin (CfLec-4) with four CRDs from Chlamys farreri was selected to investigate its possible function in innate immunity. The mRNA expression of CfLec-4 in hemocytes was significantly up-regulated (P < 0.01) after the stimulations of beta-glucan, LPS or PGN, and reached the highest expression level at 3, 6, 12 h post-stimulation, which was 27.9-, 22.6- or 47.9-fold of that in blank group, respectively. Immunohistochemistry assay with polyclonal antibody specific for CfLec-4 revealed that the endogenous CfLec-4 was mainly located in the hepatopancreas, kidney and gonad of the scallops. The recombinant CfLec-4 (rCflec-4) could bind LPS, PGN, glucan and mannose in vitro, but could not bind LTA. Furthermore, rCflec-4 displayed a broader bacteria binding spectrum towards Gram-positive bacteria Staphylococcus aureus and Micrococcus luteus as well as Gram-negative bacteria Escherichia coil, Vibrio anguillarum and fungi Pichia pastoris. Meanwhile, rCfLec-4 could significantly (P < 0.01) enhance the phagocytosis of hemocytes in vitro. The results clearly suggested that four-CRD containing CfLec-4 not only served as PRR with wider recognition spectrum, but also functioned as an opsonin participating in the clearance of invaders in scallops. It could be inferred that the diversity and complexity of CRDs in C-type lectins endowed these receptors with comprehensive recognition spectrum and multiple immune functions against complex living environment. (C) 2012 Elsevier Ltd. All rights reserved. |
| 英文摘要 | C-type lectins are a superfamily of Ca2+-dependent carbohydrate-recognition proteins consisting of at least one carbohydrate-recognition domain (CRD), which participate in nonself-recognition and clearance of invaders. In invertebrate, some multidomain C-type lectins have been identified, but their relative functions and binding mechanism are still meager. In the present study, A C-type lectin (CfLec-4) with four CRDs from Chlamys farreri was selected to investigate its possible function in innate immunity. The mRNA expression of CfLec-4 in hemocytes was significantly up-regulated (P < 0.01) after the stimulations of beta-glucan, LPS or PGN, and reached the highest expression level at 3, 6, 12 h post-stimulation, which was 27.9-, 22.6- or 47.9-fold of that in blank group, respectively. Immunohistochemistry assay with polyclonal antibody specific for CfLec-4 revealed that the endogenous CfLec-4 was mainly located in the hepatopancreas, kidney and gonad of the scallops. The recombinant CfLec-4 (rCflec-4) could bind LPS, PGN, glucan and mannose in vitro, but could not bind LTA. Furthermore, rCflec-4 displayed a broader bacteria binding spectrum towards Gram-positive bacteria Staphylococcus aureus and Micrococcus luteus as well as Gram-negative bacteria Escherichia coil, Vibrio anguillarum and fungi Pichia pastoris. Meanwhile, rCfLec-4 could significantly (P < 0.01) enhance the phagocytosis of hemocytes in vitro. The results clearly suggested that four-CRD containing CfLec-4 not only served as PRR with wider recognition spectrum, but also functioned as an opsonin participating in the clearance of invaders in scallops. It could be inferred that the diversity and complexity of CRDs in C-type lectins endowed these receptors with comprehensive recognition spectrum and multiple immune functions against complex living environment. (C) 2012 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology ; Zoology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | PATTERN-RECOGNITION ; INNATE IMMUNITY ; BINDING LECTIN ; MANDUCA-SEXTA ; PATHOGEN RECOGNITION ; COMPLEMENT PATHWAY ; SELF-RECOGNITION ; BOMBYX-MORI ; PROTEIN ; LIPOPOLYSACCHARIDE |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.dci.2012.12.002 |
| 语种 | 英语 |
| WOS记录号 | WOS:000317248900006 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16511]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, H |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Mengmeng,Wang, Lingling,Yang, Jialong,et al. A four-CRD C-type lectin from Chlamys farreri mediating nonself-recognition with broader spectrum and opsonization[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,39(4):363-369. |
| APA | Huang, Mengmeng.,Wang, Lingling.,Yang, Jialong.,Zhang, Huan.,Wang, Leilei.,...&Zhang, H.(2013).A four-CRD C-type lectin from Chlamys farreri mediating nonself-recognition with broader spectrum and opsonization.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,39(4),363-369. |
| MLA | Huang, Mengmeng,et al."A four-CRD C-type lectin from Chlamys farreri mediating nonself-recognition with broader spectrum and opsonization".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 39.4(2013):363-369. |
入库方式： OAI收割
来源：海洋研究所
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