Applications of three DNA barcodes in assorting intertidal red macroalgal flora in Qingdao, China
文献类型：期刊论文
| 作者 | Zhao Xiaobo1,2; Pang Shaojun1; Shan Tifeng1; Liu Feng1 |
| 刊名 | JOURNAL OF OCEAN UNIVERSITY OF CHINA
 |
| 出版日期 | 2013-03-01 |
| 卷号 | 12期号:1页码:139-145 |
| 关键词 | red seaweeds DNA barcoding COI ITS UPA |
| ISSN号 | 1672-5182 |
| 通讯作者 | Pang, SJ |
| 中文摘要 | This study is part of the endeavor to construct a comprehensive DNA barcoding database for common seaweeds in China. Identifications of red seaweeds, which have simple morphology and anatomy, are sometimes difficult solely depending on morphological characteristics. In recent years, DNA barcode technique has become a more and more effective tool to help solve some of the taxonomic difficulties. Some DNA markers such as COI (cytochrome oxidase subunit I) are proposed as standardized DNA barcodes for all seaweed species. In this study, COI, UPA (universal plastid amplicon, domain V of 23S rRNA), and ITS (nuclear internal transcribed spacer) were employed to analyze common species of intertidal red seaweeds in Qingdao (119.3A degrees-121A degrees E, 35.35A degrees-37.09A degrees N). The applicability of using one or a few combined barcodes to identify red seaweed species was tested. The results indicated that COI is a sensitive marker at species level. However, not all the tested species gave PCR amplification products due to lack of the universal primers. The second barcode UPA had effective universal primers but needed to be tested for the effectiveness of resolving closely related species. More than one ITS sequence types were found in some species in this investigation, which might lead to confusion in further analysis. Therefore ITS sequence is not recommended as a universal barcode for seaweeds identification. |
| 英文摘要 | This study is part of the endeavor to construct a comprehensive DNA barcoding database for common seaweeds in China. Identifications of red seaweeds, which have simple morphology and anatomy, are sometimes difficult solely depending on morphological characteristics. In recent years, DNA barcode technique has become a more and more effective tool to help solve some of the taxonomic difficulties. Some DNA markers such as COI (cytochrome oxidase subunit I) are proposed as standardized DNA barcodes for all seaweed species. In this study, COI, UPA (universal plastid amplicon, domain V of 23S rRNA), and ITS (nuclear internal transcribed spacer) were employed to analyze common species of intertidal red seaweeds in Qingdao (119.3A degrees-121A degrees E, 35.35A degrees-37.09A degrees N). The applicability of using one or a few combined barcodes to identify red seaweed species was tested. The results indicated that COI is a sensitive marker at species level. However, not all the tested species gave PCR amplification products due to lack of the universal primers. The second barcode UPA had effective universal primers but needed to be tested for the effectiveness of resolving closely related species. More than one ITS sequence types were found in some species in this investigation, which might lead to confusion in further analysis. Therefore ITS sequence is not recommended as a universal barcode for seaweeds identification. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | Oceanography |
| 类目[WOS] | Oceanography |
| 研究领域[WOS] | Oceanography |
| 关键词[WOS] | RHODOPHYTA ; NUCLEAR ; PHAEOPHYCEAE ; DIVERSITY ; SEQUENCES ; FLORIDEOPHYCEAE ; AMPLIFICATION ; GIGARTINALES ; PHYLOGENY ; TAXONOMY |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s11802-013-2052-9 |
| 语种 | 英语 |
| WOS记录号 | WOS:000313973800019 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16529]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Marine Biol Culture Collect Ctr, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao Xiaobo,Pang Shaojun,Shan Tifeng,et al. Applications of three DNA barcodes in assorting intertidal red macroalgal flora in Qingdao, China[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2013,12(1):139-145. |
| APA | Zhao Xiaobo,Pang Shaojun,Shan Tifeng,&Liu Feng.(2013).Applications of three DNA barcodes in assorting intertidal red macroalgal flora in Qingdao, China.JOURNAL OF OCEAN UNIVERSITY OF CHINA,12(1),139-145. |
| MLA | Zhao Xiaobo,et al."Applications of three DNA barcodes in assorting intertidal red macroalgal flora in Qingdao, China".JOURNAL OF OCEAN UNIVERSITY OF CHINA 12.1(2013):139-145. |
入库方式： OAI收割
来源：海洋研究所
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