Characteristics of hydrogen production by an aciduric transposon-mutagenized strain of Pantoea agglomerans BH18
文献类型：期刊论文
| 作者 | Liu, Hongyan1; Wang, Guangce1,2 |
| 刊名 | INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
 |
| 出版日期 | 2013-10-08 |
| 卷号 | 38期号:30页码:13192-13197 |
| 关键词 | Transposon-mutagenized library Mutant strain Acid tolerance Hydrogen production |
| ISSN号 | 0360-3199 |
| 通讯作者 | Wang, GC |
| 中文摘要 | Based on the generation of Pantoea agglomerans BH18 library using Tn7 transposon system, mutant screens were conducted for improvement the ability of hydrogen production of this strain. These mutants were used to test for ability of hydrogen production in the initial pH of 5.0. In contrast to wild type strain BH18, a transposon mutant, named as strain TB108, was screened for high hydrogen-producing capability and acid tolerance in the initial pH of 5.0. The factors required for hydrogen production of the aciduric transposon-mutagenized strain TB108 were determined. The mutant strain TB108 similar as wild type strain BH18 was able to produce hydrogen over a wide range of salt concentration from 0.4% to 6%. Under the marine conditions with the initial pH of 5.0 and glucose concentration of 10 g/L, the total hydrogen production of the mutant TB108 was (1.36 +/- 0.04) mol H-2/mol glucose (mean +/- SE.), increasing by 55% compared with wild type. In addition, the mutant strain TB108 could produce hydrogen using many carbon sources such as fructose, glucose, sucrose, sorbitol and so on. This result demonstrated that the mutant strain with high acid tolerance is beneficial for improvement of hydrogen production. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved. |
| 英文摘要 | Based on the generation of Pantoea agglomerans BH18 library using Tn7 transposon system, mutant screens were conducted for improvement the ability of hydrogen production of this strain. These mutants were used to test for ability of hydrogen production in the initial pH of 5.0. In contrast to wild type strain BH18, a transposon mutant, named as strain TB108, was screened for high hydrogen-producing capability and acid tolerance in the initial pH of 5.0. The factors required for hydrogen production of the aciduric transposon-mutagenized strain TB108 were determined. The mutant strain TB108 similar as wild type strain BH18 was able to produce hydrogen over a wide range of salt concentration from 0.4% to 6%. Under the marine conditions with the initial pH of 5.0 and glucose concentration of 10 g/L, the total hydrogen production of the mutant TB108 was (1.36 +/- 0.04) mol H-2/mol glucose (mean +/- SE.), increasing by 55% compared with wild type. In addition, the mutant strain TB108 could produce hydrogen using many carbon sources such as fructose, glucose, sucrose, sorbitol and so on. This result demonstrated that the mutant strain with high acid tolerance is beneficial for improvement of hydrogen production. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 学科主题 | Chemistry ; Electrochemistry ; Energy & Fuels |
| 类目[WOS] | Chemistry, Physical ; Electrochemistry ; Energy & Fuels |
| 研究领域[WOS] | Chemistry ; Electrochemistry ; Energy & Fuels |
| 关键词[WOS] | BIOHYDROGEN PRODUCTION ; SEWAGE-SLUDGE ; COW DUNG ; IMPROVEMENT ; OVEREXPRESSION ; FERMENTATION ; ISOLATE ; STARCH ; WASTE ; GENE |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.ijhydene.2013.07.065 |
| 语种 | 英语 |
| WOS记录号 | WOS:000326214200008 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16536]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin Key Lab Marine Resources & Chem, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Hongyan,Wang, Guangce. Characteristics of hydrogen production by an aciduric transposon-mutagenized strain of Pantoea agglomerans BH18[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2013,38(30):13192-13197. |
| APA | Liu, Hongyan,&Wang, Guangce.(2013).Characteristics of hydrogen production by an aciduric transposon-mutagenized strain of Pantoea agglomerans BH18.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,38(30),13192-13197. |
| MLA | Liu, Hongyan,et al."Characteristics of hydrogen production by an aciduric transposon-mutagenized strain of Pantoea agglomerans BH18".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 38.30(2013):13192-13197. |
入库方式： OAI收割
来源：海洋研究所
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