Characterization of the high cytochalasin E and rosellichalasin producing-Aspergillus sp. nov. F1 isolated from marine solar saltern in China
文献类型：期刊论文
| 作者 | Xiao, Lin1,2,3; Liu, Haizhou1; Wu, Ning1; Liu, Ming1; Wei, Jianteng1; Zhang, Yuyan1; Lin, Xiukun1 |
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 29期号:1页码:11-17 |
| 关键词 | Moderately halophilic fungus Aspergillus Cytotoxic activity Cytochalasin |
| ISSN号 | 0959-3993 |
| 通讯作者 | Lin, XK |
| 中文摘要 | A moderately halophilic fungus F1 was isolated from a marine solar saltern in Weihai, China. The identification of the fungus F1 was performed by the morphological characteristics, physiological and biochemical tests as well as phylogenetic analysis based on ITS (internal transcribed spacer)-5.8S rDNA region sequence comparison. The strain was identified as belonging to the genus Aspergillus and designated as Aspergillus sp. nov. F1. Furthermore, Aspergillus sp. nov. F1 grew well in 3-15 % (w/v) NaCl, and with increasing of salinity, the generation of secondary metabolites with cytotoxicity was also augmented. Three compounds with cytotoxicity were isolated from the ethyl acetate extract of the whole broth and mycelia of Aspergillus sp. nov. F1, and identified as ergosterol, rosellichalasin and cytochalasin E, respectively. Especially, ergosterol showed high potent cytotoxic activity to human colon cancer cell line RKO with IC50 of 3.3 +/- A 0.5 mu M. Considering the high cytochalasin production and the simple and economical fermentation of Aspergillus sp. nov. F1, the strain could be used as potential strain for large scale production of the cytochalasin E and rosellichalasin. |
| 英文摘要 | A moderately halophilic fungus F1 was isolated from a marine solar saltern in Weihai, China. The identification of the fungus F1 was performed by the morphological characteristics, physiological and biochemical tests as well as phylogenetic analysis based on ITS (internal transcribed spacer)-5.8S rDNA region sequence comparison. The strain was identified as belonging to the genus Aspergillus and designated as Aspergillus sp. nov. F1. Furthermore, Aspergillus sp. nov. F1 grew well in 3-15 % (w/v) NaCl, and with increasing of salinity, the generation of secondary metabolites with cytotoxicity was also augmented. Three compounds with cytotoxicity were isolated from the ethyl acetate extract of the whole broth and mycelia of Aspergillus sp. nov. F1, and identified as ergosterol, rosellichalasin and cytochalasin E, respectively. Especially, ergosterol showed high potent cytotoxic activity to human colon cancer cell line RKO with IC50 of 3.3 +/- A 0.5 mu M. Considering the high cytochalasin production and the simple and economical fermentation of Aspergillus sp. nov. F1, the strain could be used as potential strain for large scale production of the cytochalasin E and rosellichalasin. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | SEQUENCE ALIGNMENT ; METABOLITE ; EXTRACTS ; BACTERIA ; HALOCINS ; GROWTH ; ACTIN ; CELLS |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s11274-012-1152-9 |
| 语种 | 英语 |
| WOS记录号 | WOS:000312075300002 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16539]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, Lin,Liu, Haizhou,Wu, Ning,et al. Characterization of the high cytochalasin E and rosellichalasin producing-Aspergillus sp. nov. F1 isolated from marine solar saltern in China[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2013,29(1):11-17. |
| APA | Xiao, Lin.,Liu, Haizhou.,Wu, Ning.,Liu, Ming.,Wei, Jianteng.,...&Lin, Xiukun.(2013).Characterization of the high cytochalasin E and rosellichalasin producing-Aspergillus sp. nov. F1 isolated from marine solar saltern in China.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,29(1),11-17. |
| MLA | Xiao, Lin,et al."Characterization of the high cytochalasin E and rosellichalasin producing-Aspergillus sp. nov. F1 isolated from marine solar saltern in China".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 29.1(2013):11-17. |
入库方式： OAI收割
来源：海洋研究所
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