Cloning and characterization of a novel diacylglycerol acyltransferase from the diatom Phaeodactylum tricornutum
文献类型：期刊论文
| 作者 | Cui, Yulin2; Zheng, Guoting1,3; Li, Xueqin2,4; Lin, Hanzhi5; Jiang, Peng1; Qin, Song2 |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
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| 出版日期 | 2013-10-01 |
| 卷号 | 25期号:5页码:1509-1512 |
| ISSN号 | 0921-8971 |
| 关键词 | Acyl-CoA:diacylglycerol acyltransferase (DGAT) Heterologous expression Phaeodactylum tricornutum Triacyglycerol (TAG) |
| 通讯作者 | Jiang, P |
| 中文摘要 | In this study, a cDNA encoding a novel acyl-CoA:diacylglycerol acyltransferase (DGAT)-like protein is identified and isolated from the diatom microalga Phaeodactylum tricornutum (PtDGAT3). Analysis of the sequence reveals that ptDGAT3 cDNA encodes a protein of 504 amino acids with a molecular mass of 64.5 KDa. The putative ptDGAT3 protein has two catalytic domains: a wax ester synthase-like acyl-CoA acyltransferase domain and a bacteria-specific acyltransferase domain, which shows higher similarity to the DGAT3 of Acinetobacter calcoaceticus than reported DGAT1 or DGAT2 from high plants or algae. Its activity was confirmed by heterologous expression of PtDGAT3 in a neutral lipid-deficient quadruple mutant yeast Saccharomyces cerevisiae H1246. The recombinant yeast restored the formation of a lipid body and displayed a preference to the incorporation of unsaturated C-18 fatty acids into triacyglycerol (TAG). This is the first characterized algal DGAT3 gene, giving further evidence to the occurrence of a DGAT3-mediated TAG biosynthesis pathway. |
| 英文摘要 | In this study, a cDNA encoding a novel acyl-CoA:diacylglycerol acyltransferase (DGAT)-like protein is identified and isolated from the diatom microalga Phaeodactylum tricornutum (PtDGAT3). Analysis of the sequence reveals that ptDGAT3 cDNA encodes a protein of 504 amino acids with a molecular mass of 64.5 KDa. The putative ptDGAT3 protein has two catalytic domains: a wax ester synthase-like acyl-CoA acyltransferase domain and a bacteria-specific acyltransferase domain, which shows higher similarity to the DGAT3 of Acinetobacter calcoaceticus than reported DGAT1 or DGAT2 from high plants or algae. Its activity was confirmed by heterologous expression of PtDGAT3 in a neutral lipid-deficient quadruple mutant yeast Saccharomyces cerevisiae H1246. The recombinant yeast restored the formation of a lipid body and displayed a preference to the incorporation of unsaturated C-18 fatty acids into triacyglycerol (TAG). This is the first characterized algal DGAT3 gene, giving further evidence to the occurrence of a DGAT3-mediated TAG biosynthesis pathway. |
| 学科主题 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 关键词[WOS] | BIOSYNTHESIS |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s10811-013-9991-9 |
| 语种 | 英语 |
| WOS记录号 | WOS:000324104700026 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16544]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Expt Marine Biol Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Shandong, Peoples R China 3.Ningbo Univ, Key Lab Marine Biotechnol, Ningbo 315211, Zhejiang, Peoples R China 4.Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China 5.Rutgers State Univ, Inst Marine & Coastal Sci, New Brunswick, NJ 08901 USA |
| 推荐引用方式 GB/T 7714 | Cui, Yulin,Zheng, Guoting,Li, Xueqin,et al. Cloning and characterization of a novel diacylglycerol acyltransferase from the diatom Phaeodactylum tricornutum[J]. JOURNAL OF APPLIED PHYCOLOGY,2013,25(5):1509-1512. |
| APA | Cui, Yulin,Zheng, Guoting,Li, Xueqin,Lin, Hanzhi,Jiang, Peng,&Qin, Song.(2013).Cloning and characterization of a novel diacylglycerol acyltransferase from the diatom Phaeodactylum tricornutum.JOURNAL OF APPLIED PHYCOLOGY,25(5),1509-1512. |
| MLA | Cui, Yulin,et al."Cloning and characterization of a novel diacylglycerol acyltransferase from the diatom Phaeodactylum tricornutum".JOURNAL OF APPLIED PHYCOLOGY 25.5(2013):1509-1512. |
入库方式： OAI收割
来源：海洋研究所
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