Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity
文献类型：期刊论文
| 作者 | Jia, Pan-pan1,2; Hu, Yong-hua2; Chi, Heng2; Sun, Bo-guang2 ; Yu, Wen-gong1; Sun, Li2
|
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2013-02-01 |
| 卷号 | 34期号:2页码:514-520 |
| 关键词 | Vibrio anguillarum Flagellin Vaccine Adjuvant |
| ISSN号 | 1050-4648 |
| 通讯作者 | Sun, L |
| 中文摘要 | Vibrio anguillarum is the etiological agent of vibriosis, an aquaculture disease that affects a wide range of farmed fish. The genome of V anguillarum contains five flagellin genes, i.e. flaA,flaB,flaC,flaD, and flaE. In this study, we analyzed the vaccine potential and adjuvanticity of FlaA, FlaB, FlaD, and FlaE in a model of Japanese flounder (Paralichthys olivaceus). For this purpose, recombinant FlaA, FlaB, FlaD, and FlaE were expressed in and purified from Escherichia coli. In vivo immunogenicity analysis showed that antibodies against rFlaA, rFlaB, rFlaD, and rFlaE were detected in rat antiserum raised against live V. anguillarum, with the highest antibody level being that against rFlaB. When administered into flounder via intraperitoneal injection, rFlaA, rFlaD, and rFlaE induced comparable relative percent survival (RPS) rates, which were significantly lower than that induced by rFlaB. Specific serum antibodies were induced by all flagellins, however, the antibody level induced by rFlaB was significantly higher than those induced by other three flagellins. Compared to sera from fish vaccinated with rFlaA, rFlaD, and rFlaE, serum from fish vaccinated with rFlaB significantly reduced the infectivity of V. anguillarum against host cells. To examine the potential adjuvant effect of the flagellins, flounder were immunized with rEsa1, a D15-like surface antigen that induces protective immunity as a subunit vaccine, in the presence or absence of rFlaA, rFlaB, rFlaD, and rFlaE respectively. The results showed that rFlaE, but not other three flagellins, significantly increased the RPS of rEsa1. Compared to fish vaccinated with rEsa1, fish vaccinated with rEsa1 plus rFlaE exhibited a significantly higher level of serum antibodies and enhanced expression of the genes involved in innate and adaptive immunity. Taken together, these results indicate that FlaA, FlaB, FlaD, and FlaE have different immunological properties and, as a result, differ in vaccine and adjuvant potentials. (C) 2012 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Vibrio anguillarum is the etiological agent of vibriosis, an aquaculture disease that affects a wide range of farmed fish. The genome of V anguillarum contains five flagellin genes, i.e. flaA,flaB,flaC,flaD, and flaE. In this study, we analyzed the vaccine potential and adjuvanticity of FlaA, FlaB, FlaD, and FlaE in a model of Japanese flounder (Paralichthys olivaceus). For this purpose, recombinant FlaA, FlaB, FlaD, and FlaE were expressed in and purified from Escherichia coli. In vivo immunogenicity analysis showed that antibodies against rFlaA, rFlaB, rFlaD, and rFlaE were detected in rat antiserum raised against live V. anguillarum, with the highest antibody level being that against rFlaB. When administered into flounder via intraperitoneal injection, rFlaA, rFlaD, and rFlaE induced comparable relative percent survival (RPS) rates, which were significantly lower than that induced by rFlaB. Specific serum antibodies were induced by all flagellins, however, the antibody level induced by rFlaB was significantly higher than those induced by other three flagellins. Compared to sera from fish vaccinated with rFlaA, rFlaD, and rFlaE, serum from fish vaccinated with rFlaB significantly reduced the infectivity of V. anguillarum against host cells. To examine the potential adjuvant effect of the flagellins, flounder were immunized with rEsa1, a D15-like surface antigen that induces protective immunity as a subunit vaccine, in the presence or absence of rFlaA, rFlaB, rFlaD, and rFlaE respectively. The results showed that rFlaE, but not other three flagellins, significantly increased the RPS of rEsa1. Compared to fish vaccinated with rEsa1, fish vaccinated with rEsa1 plus rFlaE exhibited a significantly higher level of serum antibodies and enhanced expression of the genes involved in innate and adaptive immunity. Taken together, these results indicate that FlaA, FlaB, FlaD, and FlaE have different immunological properties and, as a result, differ in vaccine and adjuvant potentials. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | MUCOSAL IMMUNE-RESPONSES ; TOLL-LIKE RECEPTOR-5 ; FISH CELL-LINE ; BACTERIAL FLAGELLIN ; PARALICHTHYS-OLIVACEUS ; INNATE IMMUNITY ; EXPRESSION ; MOTILITY ; PROTEIN ; SALMONELLA |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.fsi.2012.11.039 |
| 语种 | 英语 |
| WOS记录号 | WOS:000315076100012 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16547]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Pan-pan,Hu, Yong-hua,Chi, Heng,et al. Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(2):514-520. |
| APA | Jia, Pan-pan,Hu, Yong-hua,Chi, Heng,Sun, Bo-guang,Yu, Wen-gong,&Sun, Li.(2013).Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity.FISH & SHELLFISH IMMUNOLOGY,34(2),514-520. |
| MLA | Jia, Pan-pan,et al."Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity".FISH & SHELLFISH IMMUNOLOGY 34.2(2013):514-520. |
入库方式： OAI收割
来源：海洋研究所
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