Complete genome sequence and transcription profiles of the rock bream iridovirus RBIV-C1
文献类型：期刊论文
| 作者 | Zhang, Bao-Cun1,2; Zhang, Min1; Sun, Bo-Guang1 ; Fang, Yong1,2; Xiao, Zhi-Zhong1; Sun, Li1
|
| 刊名 | DISEASES OF AQUATIC ORGANISMS
 |
| 出版日期 | 2013-06-13 |
| 卷号 | 104期号:3页码:203-+ |
| 关键词 | Iridovirus Megalocytivirus Comparative genome Oplegnathus fasciatus |
| ISSN号 | 0177-5103 |
| 通讯作者 | Sun, L |
| 中文摘要 | The family Iridoviridae consists of 5 genera of double-stranded DNA viruses, including the genus Megalocytivirus, which contains species that are important fish pathogens. In a previous study, we isolated the first rock bream iridovirus from China (RBIV-C1) and identified it as a member of the genus Megalocytivirus. In this report, we determined the complete genomic sequence of RBIV-C1 and examined its in vivo expression profiles. The genome of RBIV-C1 is 112 333 bp in length, with a GC content of 55% and a coding density of 92%. RBIV-C1 contains 4584 simple sequence repeats, 89.8% of which are distributed among coding regions. A total of 119 potential open reading frames (ORFs) were identified in RBIV-C1, including the 26 core iridovirus genes; 41 ORFs encode proteins that are predicted to be associated with essential biological functions. RBIV-C1 exhibits the highest degree of sequence conservation and colinear arrangement of genes with orange-spotted grouper iridovirus (OSGIV) and rock bream iridovirus (RBIV). The pair-wise nucleotide identities are 99.49% between RBIV-C1 and OSGIV and 98.69% between RBIV-C1 and RBIV. Compared to OSGIV, RBIV-C1 contains 11 insertions, 13 deletions, and 103 single nucleotide mutations. Whole-genome transcription analysis showed that following experimental infection of rock bream with RBIV-C1, all but 1 of the 119 ORFs were expressed at different time points and clustered into 3 hierarchical groups based on their expression patterns. These results provide new insights into the genetic nature and gene expression features of megalocytiviruses. |
| 英文摘要 | The family Iridoviridae consists of 5 genera of double-stranded DNA viruses, including the genus Megalocytivirus, which contains species that are important fish pathogens. In a previous study, we isolated the first rock bream iridovirus from China (RBIV-C1) and identified it as a member of the genus Megalocytivirus. In this report, we determined the complete genomic sequence of RBIV-C1 and examined its in vivo expression profiles. The genome of RBIV-C1 is 112 333 bp in length, with a GC content of 55% and a coding density of 92%. RBIV-C1 contains 4584 simple sequence repeats, 89.8% of which are distributed among coding regions. A total of 119 potential open reading frames (ORFs) were identified in RBIV-C1, including the 26 core iridovirus genes; 41 ORFs encode proteins that are predicted to be associated with essential biological functions. RBIV-C1 exhibits the highest degree of sequence conservation and colinear arrangement of genes with orange-spotted grouper iridovirus (OSGIV) and rock bream iridovirus (RBIV). The pair-wise nucleotide identities are 99.49% between RBIV-C1 and OSGIV and 98.69% between RBIV-C1 and RBIV. Compared to OSGIV, RBIV-C1 contains 11 insertions, 13 deletions, and 103 single nucleotide mutations. Whole-genome transcription analysis showed that following experimental infection of rock bream with RBIV-C1, all but 1 of the 119 ORFs were expressed at different time points and clustered into 3 hierarchical groups based on their expression patterns. These results provide new insights into the genetic nature and gene expression features of megalocytiviruses. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries ; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Veterinary Sciences |
| 关键词[WOS] | SINGAPORE GROUPER IRIDOVIRUS ; RED-SEA BREAM ; RANAVIRUS FAMILY IRIDOVIRIDAE ; LYMPHOCYSTIS DISEASE VIRUS ; ORANGE-SPOTTED GROUPER ; COMPLETE DNA-SEQUENCE ; INFECTIOUS SPLEEN ; PROTEOMIC ANALYSIS ; IRIDESCENT VIRUS ; FISH |
| 收录类别 | SCI |
| 原文出处 | 10.3354/dao02587 |
| 语种 | 英语 |
| WOS记录号 | WOS:000320321000003 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16549]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Bao-Cun,Zhang, Min,Sun, Bo-Guang,et al. Complete genome sequence and transcription profiles of the rock bream iridovirus RBIV-C1[J]. DISEASES OF AQUATIC ORGANISMS,2013,104(3):203-+. |
| APA | Zhang, Bao-Cun,Zhang, Min,Sun, Bo-Guang,Fang, Yong,Xiao, Zhi-Zhong,&Sun, Li.(2013).Complete genome sequence and transcription profiles of the rock bream iridovirus RBIV-C1.DISEASES OF AQUATIC ORGANISMS,104(3),203-+. |
| MLA | Zhang, Bao-Cun,et al."Complete genome sequence and transcription profiles of the rock bream iridovirus RBIV-C1".DISEASES OF AQUATIC ORGANISMS 104.3(2013):203-+. |
入库方式： OAI收割
来源：海洋研究所
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