Comprehensive Extraction of Agar and R-Phycoerythrin from Gracilaria lemaneiformis (Bangiales, Rhodophyta)
文献类型：期刊论文
| 作者 | Niu, Jianfeng1; Xu, Meiling3; Wang, Guangce1,3; Zhang, Kaiyue4; Peng, Guang1 |
| 刊名 | INDIAN JOURNAL OF GEO-MARINE SCIENCES
 |
| 出版日期 | 2013-02-01 |
| 卷号 | 42期号:1页码:21-28 |
| 关键词 | Gracilaria lemaneiformis R-phycoerythrin expanded bed adsorption ion-exchange chromatography agar |
| ISSN号 | 0379-5136 |
| 通讯作者 | Wang, GC |
| 中文摘要 | Water-soluble proteins from Gracilaria lemaneiformis were extracted and further purification for R-phycoerythrin (R-PE) was carried out. Remaining cell mass was used for agar extraction. Approximately 21% of the R-PE recovered from the crude extract and the yield of R-PE with a purification ratio >3.2 were 0,36 mg g(-1) fresh G, lemaneiformis, The yield of agar was 15,2%, with the gel strength of 895 +/- 20 g cm(-2) in a 1% gel at 30 degrees C, which showed no significant difference from those obtained from the directed agar extraction using the dried algae. Research in the experiments also revealed that R-PE could purify from the waste hydration solution of the dried alga, although its recovery and purity found to be lower than when it Was extracted from fresh algae, |
| 英文摘要 | Water-soluble proteins from Gracilaria lemaneiformis were extracted and further purification for R-phycoerythrin (R-PE) was carried out. Remaining cell mass was used for agar extraction. Approximately 21% of the R-PE recovered from the crude extract and the yield of R-PE with a purification ratio >3.2 were 0,36 mg g(-1) fresh G, lemaneiformis, The yield of agar was 15,2%, with the gel strength of 895 +/- 20 g cm(-2) in a 1% gel at 30 degrees C, which showed no significant difference from those obtained from the directed agar extraction using the dried algae. Research in the experiments also revealed that R-PE could purify from the waste hydration solution of the dried alga, although its recovery and purity found to be lower than when it Was extracted from fresh algae, |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | Oceanography |
| 类目[WOS] | Oceanography |
| 研究领域[WOS] | Oceanography |
| 关键词[WOS] | EXPANDED BED ADSORPTION ; LARGE-SCALE ISOLATION ; GEL-ELECTROPHORESIS ; B-PHYCOERYTHRIN ; RED ALGAE ; PURIFICATION ; PHYCOCYANIN ; RECOVERY ; QUALITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317453500004 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16550]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Nantong Branch, Nantong 226006, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201303, Peoples R China |
| 推荐引用方式 GB/T 7714 | Niu, Jianfeng,Xu, Meiling,Wang, Guangce,et al. Comprehensive Extraction of Agar and R-Phycoerythrin from Gracilaria lemaneiformis (Bangiales, Rhodophyta)[J]. INDIAN JOURNAL OF GEO-MARINE SCIENCES,2013,42(1):21-28. |
| APA | Niu, Jianfeng,Xu, Meiling,Wang, Guangce,Zhang, Kaiyue,&Peng, Guang.(2013).Comprehensive Extraction of Agar and R-Phycoerythrin from Gracilaria lemaneiformis (Bangiales, Rhodophyta).INDIAN JOURNAL OF GEO-MARINE SCIENCES,42(1),21-28. |
| MLA | Niu, Jianfeng,et al."Comprehensive Extraction of Agar and R-Phycoerythrin from Gracilaria lemaneiformis (Bangiales, Rhodophyta)".INDIAN JOURNAL OF GEO-MARINE SCIENCES 42.1(2013):21-28. |
入库方式： OAI收割
来源：海洋研究所
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