Expression analysis of phosphoenolpyruvate carboxykinase in Porphyra haitanensis (Rhodophyta) sporophytes and gametophytes
文献类型：期刊论文
| 作者 | He, Linwen1,2; Zhang, Xiaojuan1,2; Wang, Guangce1 |
| 刊名 | PHYCOLOGICAL RESEARCH
 |
| 出版日期 | 2013-07-01 |
| 卷号 | 61期号:3页码:172-179 |
| ISSN号 | 1322-0829 |
| 关键词 | C-4 phosphoenolpyruvate carboxykinase Porphyra haitanensis quantitative polymerase chain reaction rapid amplification of cDNA ends |
| 通讯作者 | Wang, GC |
| 中文摘要 | Phosphoenolpyruvate carboxykinase (PEPCK) catalyzes carboxylation of phosphoenolpyruvate or decarboxylation of oxaloacetate, which plays an important role in some C-4 plants and algae. The full-length cDNA of the PEPCK gene cloned from Porphyra haitanensisT. J. Chang et B. F. Zheng (Bangiales, Rhodophyta), was denoted Phpepck. It had a nucleotide sequence of 2300 bp, including a 5-untranslated region (UTR) of 147 bp, a 3-UTR of 329 bp and an open reading frame of 1824 bp. The expression of PEPCK in P.haitanensis sporophytes and gametophytes was examined at the levels of transcription, translation and enzyme activity. Both PEPCK abundance and activity were higher in sporophytes than those in gametophytes, suggesting that a C-4-like carbon-fixation mechanism might be functional in P.haitanensis sporophytes. |
| 英文摘要 | Phosphoenolpyruvate carboxykinase (PEPCK) catalyzes carboxylation of phosphoenolpyruvate or decarboxylation of oxaloacetate, which plays an important role in some C-4 plants and algae. The full-length cDNA of the PEPCK gene cloned from Porphyra haitanensisT. J. Chang et B. F. Zheng (Bangiales, Rhodophyta), was denoted Phpepck. It had a nucleotide sequence of 2300 bp, including a 5-untranslated region (UTR) of 147 bp, a 3-UTR of 329 bp and an open reading frame of 1824 bp. The expression of PEPCK in P.haitanensis sporophytes and gametophytes was examined at the levels of transcription, translation and enzyme activity. Both PEPCK abundance and activity were higher in sporophytes than those in gametophytes, suggesting that a C-4-like carbon-fixation mechanism might be functional in P.haitanensis sporophytes. |
| 学科主题 | Marine & Freshwater Biology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Marine & Freshwater Biology |
| 研究领域[WOS] | Marine & Freshwater Biology |
| 关键词[WOS] | C-4 PHOTOSYNTHESIS ; DNA-SEQUENCE ; RED ALGA ; GENE ; GLUCONEOGENESIS ; CHENOPODIACEAE ; PURIFICATION ; METABOLISM ; MECHANISM ; EVOLUTION |
| 收录类别 | SCI |
| 原文出处 | 10.1111/pre.12005 |
| 语种 | 英语 |
| WOS记录号 | WOS:000321753200002 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16567]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Linwen,Zhang, Xiaojuan,Wang, Guangce. Expression analysis of phosphoenolpyruvate carboxykinase in Porphyra haitanensis (Rhodophyta) sporophytes and gametophytes[J]. PHYCOLOGICAL RESEARCH,2013,61(3):172-179. |
| APA | He, Linwen,Zhang, Xiaojuan,&Wang, Guangce.(2013).Expression analysis of phosphoenolpyruvate carboxykinase in Porphyra haitanensis (Rhodophyta) sporophytes and gametophytes.PHYCOLOGICAL RESEARCH,61(3),172-179. |
| MLA | He, Linwen,et al."Expression analysis of phosphoenolpyruvate carboxykinase in Porphyra haitanensis (Rhodophyta) sporophytes and gametophytes".PHYCOLOGICAL RESEARCH 61.3(2013):172-179. |
入库方式： OAI收割
来源：海洋研究所
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