Expression characterization and activity analysis of a cathepsin B from Pacific abalone Haliotis discus hannai
文献类型：期刊论文
| 作者 | Qiu, Reng1,2; Liu, Xiao1; Hu, Yong-hua1; Sun, Bo-guang1 ; Sun, BG
|
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 34期号:5页码:1376-1382 |
| 关键词 | Cathepsin B Mollusk Abalone Haliotis discus Cysteine protease |
| ISSN号 | 1050-4648 |
| 通讯作者 | Sun, BG |
| 中文摘要 | Cathepsin B (EC 3.4.22.1) is a member of lysosomal cysteine protease and has a papain-like fold. In mammals, it is involved in protein degradation and other physiological processes including immune response. However, little is known about the function of cathepsin B in mollusks. In this study, we identified and analyzed a cathepsin B homolog (HdCatB) from Pacific abalone (Haliotis discus hannai), an economically important mollusk species cultured in East Asia. HdCatB is composed of 336 amino acid residues and its mature form is predicted to start at residue 86. HdCatB possesses typical domain architecture of cathepsin B and contains a propeptide region and a cysteine protease domain, the latter containing the four active site residues (Q108, C114, H282, and N302) that are conserved in many different organisms. HdCatB shares 40-60% overall sequence identities with the cathepsin Bofa number of vertebrates and invertebrates and is phylogenetically very close to mollusk cathepsin B. Quantitative real time RT-PCR analysis revealed that HdCatB expression occurred in multiple tissues and was upregulated by bacterial infection. Recombinant HdCatB purified from Escherichia coli exhibited apparent protease activity, which was optimal at 45 degrees C and pH 6.0. These results indicate that HdCatB is a bioactive protease that is likely to be implicated in the immune response of abalone during bacterial infection. (C) 2013 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Cathepsin B (EC 3.4.22.1) is a member of lysosomal cysteine protease and has a papain-like fold. In mammals, it is involved in protein degradation and other physiological processes including immune response. However, little is known about the function of cathepsin B in mollusks. In this study, we identified and analyzed a cathepsin B homolog (HdCatB) from Pacific abalone (Haliotis discus hannai), an economically important mollusk species cultured in East Asia. HdCatB is composed of 336 amino acid residues and its mature form is predicted to start at residue 86. HdCatB possesses typical domain architecture of cathepsin B and contains a propeptide region and a cysteine protease domain, the latter containing the four active site residues (Q108, C114, H282, and N302) that are conserved in many different organisms. HdCatB shares 40-60% overall sequence identities with the cathepsin Bofa number of vertebrates and invertebrates and is phylogenetically very close to mollusk cathepsin B. Quantitative real time RT-PCR analysis revealed that HdCatB expression occurred in multiple tissues and was upregulated by bacterial infection. Recombinant HdCatB purified from Escherichia coli exhibited apparent protease activity, which was optimal at 45 degrees C and pH 6.0. These results indicate that HdCatB is a bioactive protease that is likely to be implicated in the immune response of abalone during bacterial infection. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | LYSOSOMAL CYSTEINE PROTEASES ; CELL-DEATH ; IDENTIFICATION ; PURIFICATION ; OYSTER ; INHIBITORS ; DISEASES ; COMPLEX ; STRAIN ; GENES |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.fsi.2013.02.025 |
| 语种 | 英语 |
| WOS记录号 | WOS:000317870600046 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16569]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, BG |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, Reng,Liu, Xiao,Hu, Yong-hua,et al. Expression characterization and activity analysis of a cathepsin B from Pacific abalone Haliotis discus hannai[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1376-1382. |
| APA | Qiu, Reng,Liu, Xiao,Hu, Yong-hua,Sun, Bo-guang,&Sun, BG.(2013).Expression characterization and activity analysis of a cathepsin B from Pacific abalone Haliotis discus hannai.FISH & SHELLFISH IMMUNOLOGY,34(5),1376-1382. |
| MLA | Qiu, Reng,et al."Expression characterization and activity analysis of a cathepsin B from Pacific abalone Haliotis discus hannai".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1376-1382. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。