Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides
文献类型：期刊论文
| 作者 | Bao, Y. B.1; Li, L.2 ; Ye, M. X.1; Dong, Y. H.1; Jin, W. X.1; Lin, Z. H.1
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| 刊名 | GENETICS AND MOLECULAR RESEARCH
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| 出版日期 | 2013 |
| 卷号 | 12期号:2页码:1143-1154 |
| ISSN号 | 1676-5680 |
| 关键词 | Vibrio parahaemolyticus Messenger RNA expression Immune response Tegillarca granosa Glutamine synthetase |
| 通讯作者 | Lin, ZH |
| 中文摘要 | The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase ( GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa ( Tg-GS) from hemocytes by 3'-and 5'-rapid amplification of cDNA ends ( RACE)-PCR. The fulllength cDNA consisted of 1762 bp, with a 1104-bp open reading frame encoding 367 amino acids. Sequence comparison showed that Tg-GS has homology to GS of other organisms, with 79.78% identity with GS from the Pacific oyster Crassostrea gigas, 71.98% identity with GS from the zebrafish Danio rerio, and 68.96% identity with human Homo sapiens GS. A C-beta-Grasp domain and an N-catalytic domain were identified in Tg-GS, indicating that Tg-GS should be classified as a new member of the GS family. A quantitative RT-PCR assay was used to detect mRNA expression of Tg-GS in five different tissues. Higher levels of mRNA expression of GS were detected in the tissues of hemocytes and the mantle. Up- regulation of GS by challenge with the bacteria Vibrio parahaemolyticus and with bacterial wall lipopolysaccharides showed that GS plays a role in anti-bacterial immunity. We conclude that pathogen infection significantly induces expression level of Tg-GS, and that activation of GS influences the immune response of T. granosa by increasing glutamine concentration. |
| 英文摘要 | The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase ( GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa ( Tg-GS) from hemocytes by 3'-and 5'-rapid amplification of cDNA ends ( RACE)-PCR. The fulllength cDNA consisted of 1762 bp, with a 1104-bp open reading frame encoding 367 amino acids. Sequence comparison showed that Tg-GS has homology to GS of other organisms, with 79.78% identity with GS from the Pacific oyster Crassostrea gigas, 71.98% identity with GS from the zebrafish Danio rerio, and 68.96% identity with human Homo sapiens GS. A C-beta-Grasp domain and an N-catalytic domain were identified in Tg-GS, indicating that Tg-GS should be classified as a new member of the GS family. A quantitative RT-PCR assay was used to detect mRNA expression of Tg-GS in five different tissues. Higher levels of mRNA expression of GS were detected in the tissues of hemocytes and the mantle. Up- regulation of GS by challenge with the bacteria Vibrio parahaemolyticus and with bacterial wall lipopolysaccharides showed that GS plays a role in anti-bacterial immunity. We conclude that pathogen infection significantly induces expression level of Tg-GS, and that activation of GS influences the immune response of T. granosa by increasing glutamine concentration. |
| 学科主题 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 关键词[WOS] | EVOLUTIONARY RELATIONSHIPS ; GENE ; CLONING ; CDNA ; ENCODE |
| 收录类别 | SCI |
| 原文出处 | 10.4238/2013.April.10.9 |
| 语种 | 英语 |
| WOS记录号 | WOS:000320030100028 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16570]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Zhejiang Wanli Univ, Coll Biol & Environm Sci, Ningbo, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bao, Y. B.,Li, L.,Ye, M. X.,et al. Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides[J]. GENETICS AND MOLECULAR RESEARCH,2013,12(2):1143-1154. |
| APA | Bao, Y. B.,Li, L.,Ye, M. X.,Dong, Y. H.,Jin, W. X.,&Lin, Z. H..(2013).Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides.GENETICS AND MOLECULAR RESEARCH,12(2),1143-1154. |
| MLA | Bao, Y. B.,et al."Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides".GENETICS AND MOLECULAR RESEARCH 12.2(2013):1143-1154. |
入库方式： OAI收割
来源：海洋研究所
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