Expression patterns of an i-type lysozyme in the clam Meretrix meretrix along with larval development
文献类型：期刊论文
| 作者 | Yue, Xin1; Huan, Pin1 ; Xiao, Guoqiang2; Liu, Baozhong1; Liu, BZ
|
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2013-09-01 |
| 卷号 | 41期号:1页码:27-32 |
| 关键词 | Meretrix meretrix Lysozyme Larval immunity Whole mount in situ hybridization Whole mount immunofluorescence |
| ISSN号 | 0145-305X |
| 通讯作者 | Liu, BZ |
| 中文摘要 | An i-type lysozyme (MmeLys) has been proved to function in immunity of the clam Meretrix meretrix in our previous studies. In this study, the expression patterns of MmeLys mRNA and protein at four chief developmental stages of M. meretrix were analyzed, which was able to provide information about how ontogeny of immunity and, in particular, antibacterial ability occured in the bivalve. The results of real-time PCR and western blot showed that MmeLys expressions were activated in D-veligers and dramatically increased to the highest level in pediveligers. It is proposed that the expression changes at these two stages might be due to the visceral organs changes, which were related to the archenteron formation in D-veligers and the organ-restructuring in pediveligers during metamorphosis. In addition, new methods of whole mount in situ hybridization and whole mount immunofluorescence were applied to identify the MmeLys expression tissues, and these tissues (i.e. hepatopancreas, gill, mantle, mouth, velum and foot) may be involved in the immune function during development of clams. Our study is valuable to a certain extent for exploring the origin of immune functions in clams and provides new methodology for future studies on the immune ontogeny of bivalves. (C) 2013 Elsevier Ltd. All rights reserved. |
| 英文摘要 | An i-type lysozyme (MmeLys) has been proved to function in immunity of the clam Meretrix meretrix in our previous studies. In this study, the expression patterns of MmeLys mRNA and protein at four chief developmental stages of M. meretrix were analyzed, which was able to provide information about how ontogeny of immunity and, in particular, antibacterial ability occured in the bivalve. The results of real-time PCR and western blot showed that MmeLys expressions were activated in D-veligers and dramatically increased to the highest level in pediveligers. It is proposed that the expression changes at these two stages might be due to the visceral organs changes, which were related to the archenteron formation in D-veligers and the organ-restructuring in pediveligers during metamorphosis. In addition, new methods of whole mount in situ hybridization and whole mount immunofluorescence were applied to identify the MmeLys expression tissues, and these tissues (i.e. hepatopancreas, gill, mantle, mouth, velum and foot) may be involved in the immune function during development of clams. Our study is valuable to a certain extent for exploring the origin of immune functions in clams and provides new methodology for future studies on the immune ontogeny of bivalves. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology ; Zoology |
| 类目[WOS] | Immunology ; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | PENAEUS-SETIFERUS DECAPODA ; OYSTER CRASSOSTREA-GIGAS ; INNATE IMMUNE-RESPONSE ; GENE-EXPRESSION ; BIVALVE MOLLUSKS ; EASTERN OYSTER ; MANDUCA-SEXTA ; CATHEPSIN-B ; METAMORPHOSIS ; ONTOGENY |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.dci.2013.04.005 |
| 语种 | 英语 |
| WOS记录号 | WOS:000321091400004 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16571]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, BZ |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Zhejiang Mariculture Res Inst, Wenzhou 325005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yue, Xin,Huan, Pin,Xiao, Guoqiang,et al. Expression patterns of an i-type lysozyme in the clam Meretrix meretrix along with larval development[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,41(1):27-32. |
| APA | Yue, Xin,Huan, Pin,Xiao, Guoqiang,Liu, Baozhong,&Liu, BZ.(2013).Expression patterns of an i-type lysozyme in the clam Meretrix meretrix along with larval development.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,41(1),27-32. |
| MLA | Yue, Xin,et al."Expression patterns of an i-type lysozyme in the clam Meretrix meretrix along with larval development".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 41.1(2013):27-32. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。