Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation
文献类型：期刊论文
| 作者 | Chen Huaxin1; Lin Hanzhi1,2; Jiang Peng1; Li Fuchao1; Qin Song3 |
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2013-03-01 |
| 卷号 | 31期号:2页码:416-420 |
| 关键词 | chromatic acclimation electroporation genetic transformation Synechococcus sp CC9311 |
| ISSN号 | 0254-4059 |
| 通讯作者 | Jiang, P |
| 中文摘要 | Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin, kanamycin, spectinomycin, and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV, using a kanamycin resistance gene as selectable marker, was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition, the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium. |
| 英文摘要 | Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin, kanamycin, spectinomycin, and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV, using a kanamycin resistance gene as selectable marker, was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition, the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 学科主题 | Marine & Freshwater Biology ; Oceanography |
| 类目[WOS] | Limnology ; Oceanography |
| 研究领域[WOS] | Marine & Freshwater Biology ; Oceanography |
| 关键词[WOS] | CHROMATIC ADAPTATION ; MANIPULATION ; DIVERSITY ; MOTILITY ; STRAINS ; GENUS |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s00343-013-2164-5 |
| 语种 | 英语 |
| WOS记录号 | WOS:000316018300018 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16579]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen Huaxin,Lin Hanzhi,Jiang Peng,et al. Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(2):416-420. |
| APA | Chen Huaxin,Lin Hanzhi,Jiang Peng,Li Fuchao,&Qin Song.(2013).Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(2),416-420. |
| MLA | Chen Huaxin,et al."Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.2(2013):416-420. |
入库方式： OAI收割
来源：海洋研究所
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