Hemocytic immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Sun, Rui1,2; Qiu, Limei1; Yue, Feng1,2; Wang, Lingling1; Liu, Rui1; Zhou, Zhi1; Zhang, Huan1; Song, Linsheng1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 34期号:1页码:38-45 |
| 关键词 | CpG ODN Litopenaeus vannamei Apoptosis Hematopoiesis Phagocytosis |
| ISSN号 | 1050-4648 |
| 通讯作者 | Song, LS |
| 中文摘要 | CpG oligodeoxynucleotides (CpG ODNs), also called bacterial DNA or synthetic oligodeoxynucleotides, can induce apparent immunity protection against various pathogens, and they are widely used as functional immunostimulant or vaccine adjuvant in mammals. In the present study, CpG-rich plasmid pUC57-CpG was constructed and employed to stimulate the shrimp Litopenaeus vannamei, and the total hemocyte count, percentage of apoptotic hemocytes, regeneration of circulating hemocytes, the ability of phagocytosis and generation of reactive oxygen species (ROS) were measured to reveal the possible protection mechanism of CpG ODNs. After the injection of pUC57-CpG, the total hemocyte count significantly decreased (p < 0.01) to 2.56 x 10(7) cell/mL at the first day post stimulation, while the apoptosis increased (p < 0.01), which was 1.72-fold of that in control group. At the same time, the regeneration of circulating hemocytes fluctuated in a similar trend, and a significant increase was observed at the first day post stimulation. The phagocytotic activity including the percentage of phagocytosis and phagocytotic index, experienced an upward tend during the whole experimental period and the ROS level increased by 22% (p < 0.05) compared to that in the control group at first day post stimulation. These results together suggested that pUC57-CpG could promote the apoptosis and regeneration of circulating hemocytes, and enhance the phagocytosis and ROS production, which might contribute to the boosted immunity against the infection of pathogens. (c) 2012 Elsevier Ltd. All rights reserved. |
| 英文摘要 | CpG oligodeoxynucleotides (CpG ODNs), also called bacterial DNA or synthetic oligodeoxynucleotides, can induce apparent immunity protection against various pathogens, and they are widely used as functional immunostimulant or vaccine adjuvant in mammals. In the present study, CpG-rich plasmid pUC57-CpG was constructed and employed to stimulate the shrimp Litopenaeus vannamei, and the total hemocyte count, percentage of apoptotic hemocytes, regeneration of circulating hemocytes, the ability of phagocytosis and generation of reactive oxygen species (ROS) were measured to reveal the possible protection mechanism of CpG ODNs. After the injection of pUC57-CpG, the total hemocyte count significantly decreased (p < 0.01) to 2.56 x 10(7) cell/mL at the first day post stimulation, while the apoptosis increased (p < 0.01), which was 1.72-fold of that in control group. At the same time, the regeneration of circulating hemocytes fluctuated in a similar trend, and a significant increase was observed at the first day post stimulation. The phagocytotic activity including the percentage of phagocytosis and phagocytotic index, experienced an upward tend during the whole experimental period and the ROS level increased by 22% (p < 0.05) compared to that in the control group at first day post stimulation. These results together suggested that pUC57-CpG could promote the apoptosis and regeneration of circulating hemocytes, and enhance the phagocytosis and ROS production, which might contribute to the boosted immunity against the infection of pathogens. (c) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | PROTEIN PAP GENE ; SIGNAL-TRANSDUCTION ; BACTERIAL-DNA ; IN-VIVO ; CELLS ; OLIGODEOXYNUCLEOTIDES ; PHAGOCYTOSIS ; HEMATOPOIESIS ; EXPRESSION ; RECEPTOR |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.fsi.2012.09.016 |
| 语种 | 英语 |
| WOS记录号 | WOS:000314743600005 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16583]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Rui,Qiu, Limei,Yue, Feng,et al. Hemocytic immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(1):38-45. |
| APA | Sun, Rui.,Qiu, Limei.,Yue, Feng.,Wang, Lingling.,Liu, Rui.,...&Song, Linsheng.(2013).Hemocytic immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,34(1),38-45. |
| MLA | Sun, Rui,et al."Hemocytic immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 34.1(2013):38-45. |
入库方式： OAI收割
来源：海洋研究所
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