Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis
文献类型：期刊论文
| 作者 | Li, Ying Xia1,2; Wang, Guang Ce2; Niu, Jian Feng2; Gao, Zheng Quan; Chen, Chang Sheng3,4,5; Li, YX
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| 刊名 | INDIAN JOURNAL OF GEO-MARINE SCIENCES
 |
| 出版日期 | 2013-02-01 |
| 卷号 | 42期号:1页码:11-15 |
| 关键词 | Porphyra haitanesis thylakoid membranes photosystem II |
| ISSN号 | 0379-5136 |
| 通讯作者 | Li, YX |
| 中文摘要 | Thylakoid membranes were isolated and purified from gametophyte of Porphyra haitanesis by sucrose density gradient ultracentrifugation. After P. haitanesis gametophyte thylakoid membranes were solubilized with sodium dodecyl sulfate (SDS), the highly pure and active photosystem (PS) II complex was isolated and characterized by the room absorption and fluorescence emission spectra, DCIP (2, 6-Dichloroindophenol) reduction and oxygen- evolving rates. The PS II complex from P. haitanesis had the characteristic absorption peaks of chlorophyll (Chl) a (436 and 676 urn) and typical fluorescence emission peak at 685 nm (Ex = 436 nm). Acquired PS II complex exhibited high oxygen evolution (879 mu mol O-2. mg(-1) Chl. h(-1)) in the present of 2.5 mM DCIP as an artificial acceptor and was active in photoreduction of DCIP (2, 6- dichloroindophenol) by DPC (1, 5- diphenylcarbazide) at 104 ueq. mg(-1).Chl a. h(-1). Extrinsic proteins of P. haitanesis PS II consisted of 33, 20, 16, 12 kD proteins and cyt c-550 (15 kD), and were different with that of cyanobacteria and higher plants. |
| 英文摘要 | Thylakoid membranes were isolated and purified from gametophyte of Porphyra haitanesis by sucrose density gradient ultracentrifugation. After P. haitanesis gametophyte thylakoid membranes were solubilized with sodium dodecyl sulfate (SDS), the highly pure and active photosystem (PS) II complex was isolated and characterized by the room absorption and fluorescence emission spectra, DCIP (2, 6-Dichloroindophenol) reduction and oxygen- evolving rates. The PS II complex from P. haitanesis had the characteristic absorption peaks of chlorophyll (Chl) a (436 and 676 urn) and typical fluorescence emission peak at 685 nm (Ex = 436 nm). Acquired PS II complex exhibited high oxygen evolution (879 mu mol O-2. mg(-1) Chl. h(-1)) in the present of 2.5 mM DCIP as an artificial acceptor and was active in photoreduction of DCIP (2, 6- dichloroindophenol) by DPC (1, 5- diphenylcarbazide) at 104 ueq. mg(-1).Chl a. h(-1). Extrinsic proteins of P. haitanesis PS II consisted of 33, 20, 16, 12 kD proteins and cyt c-550 (15 kD), and were different with that of cyanobacteria and higher plants. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | Oceanography |
| 类目[WOS] | Oceanography |
| 研究领域[WOS] | Oceanography |
| 关键词[WOS] | EXTRINSIC PROTEINS ; THYLAKOID MEMBRANES ; CYANIDIUM-CALDARIUM ; RECONSTITUTION ; POLYPEPTIDES ; YEZOENSIS ; BINDING ; PSII |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317453500002 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16599]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, YX |
| 作者单位 | 1.Nanyang Normal Univ, Sch Life Sci & Technol, Nanyang 473061, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Nation Deep Sea Ctr State Ocean Adm, Qingdao, Peoples R China 4.Shandong Univ Technol, Sch Life Sci, Zibo, Peoples R China 5.Jimei Univ, Dept Aquaculture, Inst Fishery Biotechnol, Xiamen 361021, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Ying Xia,Wang, Guang Ce,Niu, Jian Feng,et al. Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis[J]. INDIAN JOURNAL OF GEO-MARINE SCIENCES,2013,42(1):11-15. |
| APA | Li, Ying Xia,Wang, Guang Ce,Niu, Jian Feng,Gao, Zheng Quan,Chen, Chang Sheng,&Li, YX.(2013).Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis.INDIAN JOURNAL OF GEO-MARINE SCIENCES,42(1),11-15. |
| MLA | Li, Ying Xia,et al."Isolation and Characterization of Photosystem II Complex from Red Alga Porphyra haitanesis".INDIAN JOURNAL OF GEO-MARINE SCIENCES 42.1(2013):11-15. |
入库方式： OAI收割
来源：海洋研究所
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