Macrophage migration inhibitory factor of Sciaenops ocellatus regulates immune cell trafficking and is involved in pathogen-induced immune response
文献类型：期刊论文
| 作者 | Qiu, Reng1,2; Li, Jun1,3; Xiao, Zhi-zhong1; Sun, Li1 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2013-07-01 |
| 卷号 | 40期号:3-4页码:232-239 |
| 关键词 | Macrophage migration inhibitory factor Sciaenops ocellatus Cell traffic Pathogen infection |
| ISSN号 | 0145-305X |
| 通讯作者 | Sun, L |
| 中文摘要 | Macrophage migration inhibitory factor (MIF) is a multi-functional cytokine involved in immunoregulation and inflammation. In this study, we examined the expression and biological function of a MIF, SoMIF, from red drum Sciaenops ocellatus. SoMIF is composed of 115 residues and shares 85-99% overall sequence identities with the MIF of a number of teleost. SoMIF expression was detected in a wide range of tissues and upregulated by bacterial and viral infection in a time-dependent manner. In head kidney (HK) leukocytes, pathogen infection induced SoMIF expression, and the expressed SoMIF was secreted into the extracellular milieu. Recombinant SoMIF (rSoMIF) purified from Escherichia coil inhibited the migration of both HK monocytes and lymphocytes, and this inhibitory effect was abolished by the presence of anti-rSoMIF antibodies. When rSoMIF was administered into red drum, it stimulated the production of reactive oxygen species in HK monocytes both in the presence and absence of pathogen infection. In vivo infection study showed that compared to untreated fish, fish pre-treated with rSoMIF before bacterial infection exhibited significantly lower bacterial loads in blood, kidney, spleen, and liver. Taken together, these results indicate that SoMIF is a secreted protein that regulates immune cell trafficking and is involved in pathogen-induced immune response. (C) 2013 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Macrophage migration inhibitory factor (MIF) is a multi-functional cytokine involved in immunoregulation and inflammation. In this study, we examined the expression and biological function of a MIF, SoMIF, from red drum Sciaenops ocellatus. SoMIF is composed of 115 residues and shares 85-99% overall sequence identities with the MIF of a number of teleost. SoMIF expression was detected in a wide range of tissues and upregulated by bacterial and viral infection in a time-dependent manner. In head kidney (HK) leukocytes, pathogen infection induced SoMIF expression, and the expressed SoMIF was secreted into the extracellular milieu. Recombinant SoMIF (rSoMIF) purified from Escherichia coil inhibited the migration of both HK monocytes and lymphocytes, and this inhibitory effect was abolished by the presence of anti-rSoMIF antibodies. When rSoMIF was administered into red drum, it stimulated the production of reactive oxygen species in HK monocytes both in the presence and absence of pathogen infection. In vivo infection study showed that compared to untreated fish, fish pre-treated with rSoMIF before bacterial infection exhibited significantly lower bacterial loads in blood, kidney, spleen, and liver. Taken together, these results indicate that SoMIF is a secreted protein that regulates immune cell trafficking and is involved in pathogen-induced immune response. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology ; Zoology |
| 类目[WOS] | Immunology ; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | TOLL-LIKE RECEPTOR-4 ; FACTOR MIF ; EDWARDSIELLA-TARDA ; BACTERIAL-INFECTION ; MOLECULAR-CLONING ; DANIO-RERIO ; PROTEIN ; EXPRESSION ; IDENTIFICATION ; OXIDOREDUCTASE |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.dci.2013.03.016 |
| 语种 | 英语 |
| WOS记录号 | WOS:000321091100002 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16600]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Lake Super State Univ, Sch Biol Sci, Sault Ste Marie, MI USA |
| 推荐引用方式 GB/T 7714 | Qiu, Reng,Li, Jun,Xiao, Zhi-zhong,et al. Macrophage migration inhibitory factor of Sciaenops ocellatus regulates immune cell trafficking and is involved in pathogen-induced immune response[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,40(3-4):232-239. |
| APA | Qiu, Reng,Li, Jun,Xiao, Zhi-zhong,&Sun, Li.(2013).Macrophage migration inhibitory factor of Sciaenops ocellatus regulates immune cell trafficking and is involved in pathogen-induced immune response.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,40(3-4),232-239. |
| MLA | Qiu, Reng,et al."Macrophage migration inhibitory factor of Sciaenops ocellatus regulates immune cell trafficking and is involved in pathogen-induced immune response".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 40.3-4(2013):232-239. |
入库方式： OAI收割
来源：海洋研究所
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