Megalocytivirus-induced proteins of turbot (Scophthalmus maximus): Identification and antiviral potential
文献类型：期刊论文
| 作者 | Zhang, Jian1,2; Hu, Yong-hua1; Xiao, Zhi-zhong1; Sun, Li1 |
| 刊名 | JOURNAL OF PROTEOMICS
 |
| 出版日期 | 2013-10-08 |
| 卷号 | 91页码:430-443 |
| ISSN号 | 1874-3919 |
| 关键词 | Megalocytivirus Scophthalmus maximus Proteomic Antiviral Adjuvant |
| 通讯作者 | Sun, L |
| 中文摘要 | Megalocytivirus is an important fish pathogen with a broad host range that includes turbot. In this study, proteomic analysis was conducted to examine turbot proteins modulated in expression by megalocytivirus infection. Thirty five proteins from spleen were identified to be differentially expressed at 2 days post-viral infection (dpi) and 7 dpi. Three upregulated proteins, i.e. heat shock protein 70 (Hsp70), Mx protein, and natural killer enhancing factor (NKEF), were further analyzed for potential antiviral effect. For this purpose, turbot were administered separately with the plasmids pHsp70, pMx, and pNKEF, which express Hsp70, Mx, and NKEF respectively, before megalocytivirus infection. Viral dissemination and propagation in spleen were subsequently determined. The results showed that the viral loads in fish administered with pNKEF were significantly reduced. To examine the potential of Hsp70, Mx, and NKEF as immunological adjuvant, turbot were immunized with a DNA vaccine in the presence of pHsp70, pMx, or pNKEF. Subsequent analysis showed that the presence of pNKEF and pHsp70, but not pMx, significantly reduced viral infection and enhanced fish survival. Taken together, these results indicate that NKEF exhibits antiviral property against megalocytivirus, and that both NKEF and Hsp70 may be used in DNA vaccine-based control of megalocytivirus infection. Biological significance This study provides the first proteomic picture of turbot in response to megalocytivirus infection. We demonstrated that megalocytivirus infection modulates the expression of turbot proteins associated with various cellular functions, and that one of the upregulated proteins, NKEF, exhibits antiviral effect when overexpressed in uiuo, while another upregulated protein, Hsp70, exhibits adjuvant effect when co-immunized with a DNA vaccine. These results add molecular insights into turbot immune response induced by megalocytivirus and provide candidate proteins with application potentials in the control of megalocytivirus-associated disease. (C) 2013 Elsevier B.V. All rights reserved. |
| 英文摘要 | Megalocytivirus is an important fish pathogen with a broad host range that includes turbot. In this study, proteomic analysis was conducted to examine turbot proteins modulated in expression by megalocytivirus infection. Thirty five proteins from spleen were identified to be differentially expressed at 2 days post-viral infection (dpi) and 7 dpi. Three upregulated proteins, i.e. heat shock protein 70 (Hsp70), Mx protein, and natural killer enhancing factor (NKEF), were further analyzed for potential antiviral effect. For this purpose, turbot were administered separately with the plasmids pHsp70, pMx, and pNKEF, which express Hsp70, Mx, and NKEF respectively, before megalocytivirus infection. Viral dissemination and propagation in spleen were subsequently determined. The results showed that the viral loads in fish administered with pNKEF were significantly reduced. To examine the potential of Hsp70, Mx, and NKEF as immunological adjuvant, turbot were immunized with a DNA vaccine in the presence of pHsp70, pMx, or pNKEF. Subsequent analysis showed that the presence of pNKEF and pHsp70, but not pMx, significantly reduced viral infection and enhanced fish survival. Taken together, these results indicate that NKEF exhibits antiviral property against megalocytivirus, and that both NKEF and Hsp70 may be used in DNA vaccine-based control of megalocytivirus infection. |
| 学科主题 | Biochemistry & Molecular Biology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | RED-SEA BREAM ; FLOUNDER PARALICHTHYS-OLIVACEUS ; LARGE YELLOW CROAKER ; RECEPTOR-MEDIATED ENDOCYTOSIS ; TROUT ONCORHYNCHUS-MYKISS ; ANTIGEN-PRESENTING CELLS ; COMPLETE GENOME SEQUENCE ; IRIDOVIRUS-LIKE AGENT ; NATURAL-KILLER-CELLS ; IN-VITRO INHIBITION |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.jprot.2013.07.033 |
| 语种 | 英语 |
| WOS记录号 | WOS:000327906000034 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16602]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jian,Hu, Yong-hua,Xiao, Zhi-zhong,et al. Megalocytivirus-induced proteins of turbot (Scophthalmus maximus): Identification and antiviral potential[J]. JOURNAL OF PROTEOMICS,2013,91:430-443. |
| APA | Zhang, Jian,Hu, Yong-hua,Xiao, Zhi-zhong,&Sun, Li.(2013).Megalocytivirus-induced proteins of turbot (Scophthalmus maximus): Identification and antiviral potential.JOURNAL OF PROTEOMICS,91,430-443. |
| MLA | Zhang, Jian,et al."Megalocytivirus-induced proteins of turbot (Scophthalmus maximus): Identification and antiviral potential".JOURNAL OF PROTEOMICS 91(2013):430-443. |
入库方式： OAI收割
来源：海洋研究所
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