Mere15, a novel polypeptide from Meretrix meretrix, inhibits adhesion, migration and invasion of human lung cancer A549 cells via down-regulating MMPs
文献类型：期刊论文
| 作者 | Wang, Hui1,3 ; Wei, Jianteng1,3; Wu, Ning1; Liu, Ming1; Wang, Cuicui3; Zhang, Yuyan1; Wang, Fengxia1; Liu, Haizhou1; Lin, Xiukun1,2
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| 刊名 | PHARMACEUTICAL BIOLOGY
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| 出版日期 | 2013-02-01 |
| 卷号 | 51期号:2页码:145-151 |
| 关键词 | Anticancer cancer metastasis transwell assay gelatin zymography RT-PCR |
| ISSN号 | 1388-0209 |
| 通讯作者 | Lin, XK |
| 中文摘要 | Context: Mere15 is a novel antitumor polypeptide purified from Meretrix meretrix Linn. (Veneridae). Previous studies have shown that the polypeptide induced cell death via intrinsic mitochondrial pathway. Objective: In the present study, the effects of Mere15 on cell adhesion, migration, invasion, as well as secretion and expression of matrix metalloproteinases (MMPs) were studied in human lung adenocarcinoma A549 cells. Materials and methods: The effect of Mere15 on cell adhesion, migration and invasion were studied by cell adhesion and transwell assay. The expression of MMPs was determined by gelatin zymography and RT-PCR analysis. Results: The ability of cell adhesion was decreased by 86.74% at the concentration of 12.0 mu g/mL of Mere15. And the migration and invasion of A549 cells were decreased with the inhibition ratio of, 69.22 and 53.84% when treated with 15.0 mu g/mL of Mere15. Further study also revealed that treatment of the cancer cells with Mere15 (15.0 mu g/mL), the secretion of MMP-2 and MMP-9 were down-regulated with the inhibition ratio of 72.00 and 93.24% and the inhibition rate of mRNA expression of MMP-2 and MMP-9 was 57.54 and 91.22%, respectively. Discussion and conclusion: The study demonstrates that Mere15 inhibits tumor growth via both pro-apoptotic and antimetastasis pathways, and the polypeptide has potential to be developed as a multi-target therapeutic agent for the treatment of human lung cancer. |
| 英文摘要 | Context: Mere15 is a novel antitumor polypeptide purified from Meretrix meretrix Linn. (Veneridae). Previous studies have shown that the polypeptide induced cell death via intrinsic mitochondrial pathway. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Plant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy |
| 类目[WOS] | Plant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Plant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy |
| 关键词[WOS] | EXPRESSION ; PROLIFERATION ; GROWTH ; ACID |
| 收录类别 | SCI |
| 原文出处 | 10.3109/13880209.2012.715169 |
| 语种 | 英语 |
| WOS记录号 | WOS:000313676300002 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16603]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Capital Med Univ, Dept Pharmacol, Beijing, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hui,Wei, Jianteng,Wu, Ning,et al. Mere15, a novel polypeptide from Meretrix meretrix, inhibits adhesion, migration and invasion of human lung cancer A549 cells via down-regulating MMPs[J]. PHARMACEUTICAL BIOLOGY,2013,51(2):145-151. |
| APA | Wang, Hui.,Wei, Jianteng.,Wu, Ning.,Liu, Ming.,Wang, Cuicui.,...&Lin, Xiukun.(2013).Mere15, a novel polypeptide from Meretrix meretrix, inhibits adhesion, migration and invasion of human lung cancer A549 cells via down-regulating MMPs.PHARMACEUTICAL BIOLOGY,51(2),145-151. |
| MLA | Wang, Hui,et al."Mere15, a novel polypeptide from Meretrix meretrix, inhibits adhesion, migration and invasion of human lung cancer A549 cells via down-regulating MMPs".PHARMACEUTICAL BIOLOGY 51.2(2013):145-151. |
入库方式： OAI收割
来源：海洋研究所
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