Molecular phylogenetic analyses of Ulva (Chlorophyta, Ulvophyceae) mats in the Xiangshan Bay of China using high-resolution DNA markers
文献类型：期刊论文
| 作者 | Liu, Feng; Pang, Shaojun; Zhao, Xiaobo |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2013-10-01 |
| 卷号 | 25期号:5页码:1287-1295 |
| 关键词 | Microscopic propagule Ulva mat Ulva prolifera Ulva flexuosa High-resolution DNA markers |
| ISSN号 | 0921-8971 |
| 通讯作者 | Pang, SJ |
| 中文摘要 | Intertidal Ulva mats occur annually in winter and spring in the Xiangshan Bay (29A degrees 26'aEuro"29A degrees 34' N, 121A degrees 27'aEuro"121A degrees 50' E) of China. Thousands of tons of Ulva biomass have been harvested as edible seaweeds for human consumption for several decades in this region. This investigation was designed to quantify Ulva microscopic propagules associated with the mat, identify species composition, and to analyze intra-species relationships using three molecular markers. Phylogenetic analysis based on the nuclear encoded rDNA internal transcribed spacer region, the plastid encoded large subunit of the ribulose 1,5-bisphosphate carboxylase gene, and the 5S rDNA spacer region showed that the mat was principally composed of Ulva prolifera and Ulva flexuosa. Their propagules were detected in both the water column and sediment. Based on phylogenetic analyses of the 5S rDNA spacer region, mat samples of U. prolifera and U. flexuosa were genetically distinct from the green tide samples in the Yellow Sea and U. flexuosa samples from Jiangsu coasts, respectively, revealing that isolated geographical position of the Xiangshan Bay might result in the maintenance of a distinct Ulva population. The results demonstrate that high-resolution DNA markers have great potential in identification and discrimination at and below the species level. |
| 英文摘要 | Intertidal Ulva mats occur annually in winter and spring in the Xiangshan Bay (29A degrees 26'aEuro"29A degrees 34' N, 121A degrees 27'aEuro"121A degrees 50' E) of China. Thousands of tons of Ulva biomass have been harvested as edible seaweeds for human consumption for several decades in this region. This investigation was designed to quantify Ulva microscopic propagules associated with the mat, identify species composition, and to analyze intra-species relationships using three molecular markers. Phylogenetic analysis based on the nuclear encoded rDNA internal transcribed spacer region, the plastid encoded large subunit of the ribulose 1,5-bisphosphate carboxylase gene, and the 5S rDNA spacer region showed that the mat was principally composed of Ulva prolifera and Ulva flexuosa. Their propagules were detected in both the water column and sediment. Based on phylogenetic analyses of the 5S rDNA spacer region, mat samples of U. prolifera and U. flexuosa were genetically distinct from the green tide samples in the Yellow Sea and U. flexuosa samples from Jiangsu coasts, respectively, revealing that isolated geographical position of the Xiangshan Bay might result in the maintenance of a distinct Ulva population. The results demonstrate that high-resolution DNA markers have great potential in identification and discrimination at and below the species level. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 关键词[WOS] | PROPAGULE BANKS ; GREEN TIDES ; FRESH-WATER ; ENTEROMORPHA ; SEQUENCES ; GENERA ; SEA ; MORPHOLOGY ; DIVERSITY ; LAMINARIA |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s10811-012-9955-5 |
| 语种 | 英语 |
| WOS记录号 | WOS:000324104700003 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16607]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | Acad Sinica, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Feng,Pang, Shaojun,Zhao, Xiaobo. Molecular phylogenetic analyses of Ulva (Chlorophyta, Ulvophyceae) mats in the Xiangshan Bay of China using high-resolution DNA markers[J]. JOURNAL OF APPLIED PHYCOLOGY,2013,25(5):1287-1295. |
| APA | Liu, Feng,Pang, Shaojun,&Zhao, Xiaobo.(2013).Molecular phylogenetic analyses of Ulva (Chlorophyta, Ulvophyceae) mats in the Xiangshan Bay of China using high-resolution DNA markers.JOURNAL OF APPLIED PHYCOLOGY,25(5),1287-1295. |
| MLA | Liu, Feng,et al."Molecular phylogenetic analyses of Ulva (Chlorophyta, Ulvophyceae) mats in the Xiangshan Bay of China using high-resolution DNA markers".JOURNAL OF APPLIED PHYCOLOGY 25.5(2013):1287-1295. |
入库方式： OAI收割
来源：海洋研究所
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