Phenotypic characterization, virulence, and immunogenicity of Edwardsiella tarda LSE40 aroA mutant
文献类型：期刊论文
| 作者 | Mo, Zhao-Lan1; Li, Jie1 ; Li, Gui-Yang1; Xiao, Peng2
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| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2013-07-01 |
| 卷号 | 97期号:14页码:6325-6335 |
| 关键词 | Edwardsiella tarda aroA Phenotype Protein secretion Virulence Immunogenecity |
| ISSN号 | 0175-7598 |
| 通讯作者 | Mo, ZL |
| 中文摘要 | Bacterial aro mutants are frequently used as live attenuated vaccines for domestic animals. In this study, we characterized Edwardsiella tarda strain LSE40 with a deletion in the aroA gene. In addition to autotrophy, the aroA mutant appeared to have delayed cell division and reductions in its swarming motility, biofilm formation, and production of translocator proteins in the type III secretory system. The mutant exhibited high virulence attenuation in turbot fish, Scophthalmus maximus (L.), where the 50 % lethal dose increased by more than 3 log(10) via intraperitoneal (i.p.) injection and by > 2 log(10) via immersion exposure compared with the wild-type parent strain. A tissue persistence study showed that the mutant retained the ability to invade and spread in turbot and viable cells could be detected up to 28 days after i.p. infection and 21 days after immersion exposure. These results suggested a pleiotropic role for aroA in the physiological behavior of E. tarda. Turbot exhibited a good humoral response and the enhanced expression of innate immune factors, interleukin 1 beta and lysozyme, when vaccinated with aroA mutant at 10(5) CFU via i.p. injection and at 10(8) CFU via immersion exposure. However, the aroA mutant did not provide effective protection for turbot against edwardsiellosis following i.p. vaccination at doses of 10(4)-10(6) CFU or immersion vaccination at doses of 10(6)-10(8) CFU ml(-1). We hypothesized that the aroA mutant did not trigger an appropriate T cell-immune response in turbot against infection of E. tarda. |
| 英文摘要 | Bacterial aro mutants are frequently used as live attenuated vaccines for domestic animals. In this study, we characterized Edwardsiella tarda strain LSE40 with a deletion in the aroA gene. In addition to autotrophy, the aroA mutant appeared to have delayed cell division and reductions in its swarming motility, biofilm formation, and production of translocator proteins in the type III secretory system. The mutant exhibited high virulence attenuation in turbot fish, Scophthalmus maximus (L.), where the 50 % lethal dose increased by more than 3 log(10) via intraperitoneal (i.p.) injection and by > 2 log(10) via immersion exposure compared with the wild-type parent strain. A tissue persistence study showed that the mutant retained the ability to invade and spread in turbot and viable cells could be detected up to 28 days after i.p. infection and 21 days after immersion exposure. These results suggested a pleiotropic role for aroA in the physiological behavior of E. tarda. Turbot exhibited a good humoral response and the enhanced expression of innate immune factors, interleukin 1 beta and lysozyme, when vaccinated with aroA mutant at 10(5) CFU via i.p. injection and at 10(8) CFU via immersion exposure. However, the aroA mutant did not provide effective protection for turbot against edwardsiellosis following i.p. vaccination at doses of 10(4)-10(6) CFU or immersion vaccination at doses of 10(6)-10(8) CFU ml(-1). We hypothesized that the aroA mutant did not trigger an appropriate T cell-immune response in turbot against infection of E. tarda. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | III SECRETION SYSTEM ; SALMONELLA-TYPHIMURIUM ; AEROMONAS-SALMONICIDA ; ATTENUATED VACCINE ; BIOFILM FORMATION ; FISH ; LIVE ; IDENTIFICATION ; GENES ; FURUNCULOSIS |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s00253-013-4813-1 |
| 语种 | 英语 |
| WOS记录号 | WOS:000321121800020 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16612]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mo, Zhao-Lan,Li, Jie,Li, Gui-Yang,et al. Phenotypic characterization, virulence, and immunogenicity of Edwardsiella tarda LSE40 aroA mutant[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(14):6325-6335. |
| APA | Mo, Zhao-Lan,Li, Jie,Li, Gui-Yang,&Xiao, Peng.(2013).Phenotypic characterization, virulence, and immunogenicity of Edwardsiella tarda LSE40 aroA mutant.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(14),6325-6335. |
| MLA | Mo, Zhao-Lan,et al."Phenotypic characterization, virulence, and immunogenicity of Edwardsiella tarda LSE40 aroA mutant".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.14(2013):6325-6335. |
入库方式： OAI收割
来源：海洋研究所
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