Preparation of D-glucosamine by hydrolysis of chitosan with chitosanase and beta-D-glucosaminidase
文献类型：期刊论文
| 作者 | Sun, Yuying1; Zhang, Jiquan2; Wu, Shengjun1; Wang, Shujun1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2013-10-01 |
| 卷号 | 61页码:160-163 |
| 关键词 | Chitosanase beta-D-Glucosaminidase D-Glucosamine |
| ISSN号 | 0141-8130 |
| 通讯作者 | Zhang, JQ |
| 中文摘要 | Crude enzymes including chitosanase and beta-D-glucosaminidase were obtained by centrifugal separation from the fermentation broth of Microbacterium sp. OU01. Then the crude enzymes are used to hydrolyze chitosan for producing D-glucosamine (GlcN). The effects of temperature, pH, substrate concentration, the ratio of enzyme to chitosan, and hydrolysis time on the productivity of GlcN were discussed. The experimental result showed that the optimal conditions were temperature 50 degrees C, pH 5.8, substrate concentration 20 mg/mL, the optimum ratio of enzyme to chitosan 1.5 U/60 mg. Under above conditions, chitosan was completely hydrolyzed in 5 h. These results provide a scientific material for the optimization process of enzymatic production of GlcN. What is more, thin layer chromatography and high performance liquid chromatography were used to analyze hydrolytic product, which was proved to be GlcN. (C) 2013 Elsevier B.V. All rights reserved. |
| 英文摘要 | Crude enzymes including chitosanase and beta-D-glucosaminidase were obtained by centrifugal separation from the fermentation broth of Microbacterium sp. OU01. Then the crude enzymes are used to hydrolyze chitosan for producing D-glucosamine (GlcN). The effects of temperature, pH, substrate concentration, the ratio of enzyme to chitosan, and hydrolysis time on the productivity of GlcN were discussed. The experimental result showed that the optimal conditions were temperature 50 degrees C, pH 5.8, substrate concentration 20 mg/mL, the optimum ratio of enzyme to chitosan 1.5 U/60 mg. Under above conditions, chitosan was completely hydrolyzed in 5 h. These results provide a scientific material for the optimization process of enzymatic production of GlcN. What is more, thin layer chromatography and high performance liquid chromatography were used to analyze hydrolytic product, which was proved to be GlcN. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ACETYL-D-GLUCOSAMINE ; PURIFICATION ; DEGRADATION ; CHITIN ; ACID |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.ijbiomac.2013.06.033 |
| 语种 | 英语 |
| WOS记录号 | WOS:000326660700024 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16617]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yuying,Zhang, Jiquan,Wu, Shengjun,et al. Preparation of D-glucosamine by hydrolysis of chitosan with chitosanase and beta-D-glucosaminidase[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2013,61:160-163. |
| APA | Sun, Yuying,Zhang, Jiquan,Wu, Shengjun,&Wang, Shujun.(2013).Preparation of D-glucosamine by hydrolysis of chitosan with chitosanase and beta-D-glucosaminidase.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,61,160-163. |
| MLA | Sun, Yuying,et al."Preparation of D-glucosamine by hydrolysis of chitosan with chitosanase and beta-D-glucosaminidase".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 61(2013):160-163. |
入库方式： OAI收割
来源：海洋研究所
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