Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence
文献类型：期刊论文
| 作者 | Hao, Bin1,2; Mo, Zhao-Lan1,3; Xiao, Peng1; Pan, Hai-Jian1,2; Lan, Xin1,2; Li, Gui-Yang1,3 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2013-03-01 |
| 卷号 | 97期号:6页码:2575-2585 |
| 关键词 | Vibrio anguillarum RpoN (sigma(54)) Biofilm Exopolysaccharides Metalloproteases Virulence |
| ISSN号 | 0175-7598 |
| 通讯作者 | Mo, ZL |
| 中文摘要 | The sigma factor sigma(54) (RpoN) is an important regulator of bacterial response to environmental stresses. Here, we demonstrate the roles of RpoN in Vibrio anguillarum M3 by comparative investigation of physiological phenotypes and virulence of the wild-type, an rpoN mutant, and an rpoN complemented strain. Disruption of rpoN was found to decrease biofilm formation, production of exopolysaccharides, and production of the metalloproteases EmpA and PrtV. Injection experiments in fish showed that the M3 Delta rpoN mutant was attenuated in virulence when administrated either by intramuscular injection or by immersion challenge. Slower proliferation of the mutant in fish was also observed. Complementation of the mutant strain with rpoN restored some of the phenotypes to wild-type levels. RpoN was involved in regulation of some virulence-associated genes, as shown by real-time quantitative reverse PCR analysis. These results revealed a pleiotropic regulatory role of RpoN in biofilm formation, production of proteases and exopolysaccharides, and virulence in V. anguillarum M3. |
| 英文摘要 | The sigma factor sigma(54) (RpoN) is an important regulator of bacterial response to environmental stresses. Here, we demonstrate the roles of RpoN in Vibrio anguillarum M3 by comparative investigation of physiological phenotypes and virulence of the wild-type, an rpoN mutant, and an rpoN complemented strain. Disruption of rpoN was found to decrease biofilm formation, production of exopolysaccharides, and production of the metalloproteases EmpA and PrtV. Injection experiments in fish showed that the M3 Delta rpoN mutant was attenuated in virulence when administrated either by intramuscular injection or by immersion challenge. Slower proliferation of the mutant in fish was also observed. Complementation of the mutant strain with rpoN restored some of the phenotypes to wild-type levels. RpoN was involved in regulation of some virulence-associated genes, as shown by real-time quantitative reverse PCR analysis. These results revealed a pleiotropic regulatory role of RpoN in biofilm formation, production of proteases and exopolysaccharides, and virulence in V. anguillarum M3. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | MEDIATED IRON UPTAKE ; ESCHERICHIA-COLI ; PSEUDOMONAS-AERUGINOSA ; CHEMOTACTIC MOTILITY ; HEMOLYSIN GENE ; UPTAKE SYSTEM ; METALLOPROTEASE ; IDENTIFICATION ; EXPRESSION ; COLONIZATION |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s00253-012-4372-x |
| 语种 | 英语 |
| WOS记录号 | WOS:000315650100027 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16621]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Bin,Mo, Zhao-Lan,Xiao, Peng,et al. Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(6):2575-2585. |
| APA | Hao, Bin,Mo, Zhao-Lan,Xiao, Peng,Pan, Hai-Jian,Lan, Xin,&Li, Gui-Yang.(2013).Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(6),2575-2585. |
| MLA | Hao, Bin,et al."Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.6(2013):2575-2585. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。