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Screening and hydrogen-producing characters of a highly efficient H-2-producing mutant of Rhodovulum sulfidophilum P5

文献类型：期刊论文

作者Cai, Jinling1; Wang, Guangce1,2
刊名BIORESOURCE TECHNOLOGY
出版日期2013-08-01
卷号142页码:18-25
关键词Biological hydrogen production Transposon Rhodovulum sulfidophilum Reduced light absorption
ISSN号0960-8524
通讯作者Cai, JL
中文摘要In this study, transposon mutagenesis technology was utilized to enhance the hydrogen production capability of a wild marine photosynthetic bacterium Rhodovulum sulfidophilum P5. A mutant strain TH-253 that exhibited high hydrogen yield and weaker light absorption ability was screened. Under strong light conditions, the mutant produced more hydrogen than that of the WT. Under optimum light intensity (120 mu mol photons/m(2) s), the mutant achieved its highest hydrogen yield (1436 +/- 44 mL H-2/L, about 3.21 +/- 0.10 mol H-2/mol acetate), which was 40.37% higher that of the WT. In continuous operation mode, the hydrogen yield (3.59 +/- 0.11 mol H-2/mol acetate) and average hydrogen production rate (16.91 +/- 0.46 mL H-2/L h) of the mutant were 43.40% and 45.07% higher than those of the WT, respectively. The mutant strain TH-253 may be used as an appropriate starting strain for future photosynthesis-based large scale hydrogen production. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
英文摘要In this study, transposon mutagenesis technology was utilized to enhance the hydrogen production capability of a wild marine photosynthetic bacterium Rhodovulum sulfidophilum P5. A mutant strain TH-253 that exhibited high hydrogen yield and weaker light absorption ability was screened. Under strong light conditions, the mutant produced more hydrogen than that of the WT. Under optimum light intensity (120 mu mol photons/m(2) s), the mutant achieved its highest hydrogen yield (1436 +/- 44 mL H-2/L, about 3.21 +/- 0.10 mol H-2/mol acetate), which was 40.37% higher that of the WT. In continuous operation mode, the hydrogen yield (3.59 +/- 0.11 mol H-2/mol acetate) and average hydrogen production rate (16.91 +/- 0.46 mL H-2/L h) of the mutant were 43.40% and 45.07% higher than those of the WT, respectively. The mutant strain TH-253 may be used as an appropriate starting strain for future photosynthesis-based large scale hydrogen production. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
学科主题Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
类目[WOS]Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]RHODOPSEUDOMONAS-FAECALIS RLD-53 ; RHODOBACTER-SPHAEROIDES OU001 ; PHOTOSYNTHETIC BACTERIUM ; DEFICIENT MUTANT ; BIOHYDROGEN PRODUCTION ; RHODOSPIRILLUM-RUBRUM ; REDUCED PIGMENT ; CAPSULATUS ; PHOTO ; ACETATE
收录类别SCI
原文出处10.1016/j.biortech.2013.05.009
语种英语
WOS记录号WOS:000322292800003
公开日期2014-07-17
源URL[http://ir.qdio.ac.cn/handle/337002/16624]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin Key Lab Marine Resources & Chem, Tianjin 300457, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Cai, Jinling,Wang, Guangce. Screening and hydrogen-producing characters of a highly efficient H-2-producing mutant of Rhodovulum sulfidophilum P5[J]. BIORESOURCE TECHNOLOGY,2013,142:18-25.
APA Cai, Jinling,&Wang, Guangce.(2013).Screening and hydrogen-producing characters of a highly efficient H-2-producing mutant of Rhodovulum sulfidophilum P5.BIORESOURCE TECHNOLOGY,142,18-25.
MLA Cai, Jinling,et al."Screening and hydrogen-producing characters of a highly efficient H-2-producing mutant of Rhodovulum sulfidophilum P5".BIORESOURCE TECHNOLOGY 142(2013):18-25.

入库方式： OAI收割

来源：海洋研究所

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