Screening of genes regulated by relish in Chinese shrimp Fenneropenaeus chinensis
文献类型：期刊论文
| 作者 | Wang, Dongdong1,2; Li, Shihao1; Li, Fuhua1 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2013-10-01 |
| 卷号 | 41期号:2页码:209-216 |
| 关键词 | Relish RNAi SSH Shrimp immunity |
| ISSN号 | 0145-305X |
| 通讯作者 | Li, FH |
| 中文摘要 | Relish is a key NF-kappa B transcription factor in the innate immunity. Learning the function of Relish in regulating the related genes of shrimp will be helpful to understand the shrimp immunity. In the present study, RNA interference (RNAi) and suppression subtractive hybridization (SSH) techniques were combined together to identify the genes regulated by Relish. A forward SSH library represents the genes whose transcription was regulated by Relish, and the reverse SSH library represents the genes whose transcription was up-regulated after Relish was silenced in shrimp responsive to Vibrio anguillarium (VA) stimulation. In the forward library, 43 unique genes were identified, and in the reverse library, 57 genes were identified. The expression of ten differentially expressed genes, including early cuticle protein5 (ECP5), Toll-like receptor protein (TLRP), antiviral factor (AV), C-type lectin receptor (CLR), thrombospondin (TSP), S-adenosylmethionine synthetase (SAMS), carcinolectin 5b-5 (CL5b-5), QM protein (QMP), heat shock protein 67B2 (HSP67B2), and Thioredoxin-related protein 14 (TRP14) were further confirmed by real-time PCR. The present data provides us a wide view to understand the function of Relish gene in the innate immunity of shrimp. (C) 2013 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Relish is a key NF-kappa B transcription factor in the innate immunity. Learning the function of Relish in regulating the related genes of shrimp will be helpful to understand the shrimp immunity. In the present study, RNA interference (RNAi) and suppression subtractive hybridization (SSH) techniques were combined together to identify the genes regulated by Relish. A forward SSH library represents the genes whose transcription was regulated by Relish, and the reverse SSH library represents the genes whose transcription was up-regulated after Relish was silenced in shrimp responsive to Vibrio anguillarium (VA) stimulation. In the forward library, 43 unique genes were identified, and in the reverse library, 57 genes were identified. The expression of ten differentially expressed genes, including early cuticle protein5 (ECP5), Toll-like receptor protein (TLRP), antiviral factor (AV), C-type lectin receptor (CLR), thrombospondin (TSP), S-adenosylmethionine synthetase (SAMS), carcinolectin 5b-5 (CL5b-5), QM protein (QMP), heat shock protein 67B2 (HSP67B2), and Thioredoxin-related protein 14 (TRP14) were further confirmed by real-time PCR. The present data provides us a wide view to understand the function of Relish gene in the innate immunity of shrimp. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology ; Zoology |
| 类目[WOS] | Immunology ; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | BLACK TIGER SHRIMP ; SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; S-ADENOSYLMETHIONINE SYNTHETASE ; ANTIMICROBIAL PEPTIDE GENES ; IMMUNE-RELATED GENES ; SPOT SYNDROME VIRUS ; NF-KAPPA-B ; PENAEUS-MONODON ; LITOPENAEUS-VANNAMEI ; EXPRESSION PROFILES |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.dci.2013.06.003 |
| 语种 | 英语 |
| WOS记录号 | WOS:000323870800011 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16625]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Dongdong,Li, Shihao,Li, Fuhua. Screening of genes regulated by relish in Chinese shrimp Fenneropenaeus chinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,41(2):209-216. |
| APA | Wang, Dongdong,Li, Shihao,&Li, Fuhua.(2013).Screening of genes regulated by relish in Chinese shrimp Fenneropenaeus chinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,41(2),209-216. |
| MLA | Wang, Dongdong,et al."Screening of genes regulated by relish in Chinese shrimp Fenneropenaeus chinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 41.2(2013):209-216. |
入库方式： OAI收割
来源：海洋研究所
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