Selection of Vibrio harveyi-resistant Litopenaeus vannamei via a three-round challenge selection with a pathogenic strain of V. harveyi
文献类型：期刊论文
| 作者 | Huang, Hai-Hong1; Liu, Xiao-Lin1; Xiang, Jian-Hai2 ; Wang, Ping3
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2013-08-01 |
| 卷号 | 35期号:2页码:328-333 |
| 关键词 | Vibrio harveyi Litopenaeus vannamei Challenge Resistance Selection |
| ISSN号 | 1050-4648 |
| 通讯作者 | Liu, XL |
| 中文摘要 | To obtain Vibrio harveyi-resistant Litopenaeus vannamei shrimp used for study on immune response of shrimp avoid vibriosis, a three-round challenge selection procedure was applied. In this procedure, resistant shrimp were selected gradually via three rounds challenge experiment with a pathogenic strain of V harveyi at a median and controllable lethal dose of 96-h LD50 (the median lethal dose). After this procedure, the cumulative mortality of selected shrimp during 96 h after injection of V. harveyi at 2.0 x 10(6) cfu shrimp(-1) significantly decreased from 93.3% to 26.7%, the hours of beginning of death and the hours of attaining of the maximum cumulative mortality of shrimp prolonged from 4 h and 10 h to 8 h and 24 h, respectively. The LD50 of 6 h, 12 h, 24 h, 48 h and 96 h of selected shrimp significantly increased to 1.4 +/- 0.1 x 10(7) (p < 0.01), 5.5 +/- 0.4 x 10(6) (p < 0.01), 3.1 +/- 0.2 x 10(6) (p < 0.01), 2.7 +/- 0.1 x 10(6) (p < 0.01) and 2.7 +/- 0.1 x 10(6) cfu shrimp(-1) (p < 0.01), about 15.9, 15.3, 9.4, 10.0 and 10.4 times of that of normal shrimp, respectively. In conclusion, the resistance of shrimp to Vibrio significantly increased after the three-round challenge selection procedure. (C) 2013 Elsevier Ltd. All rights reserved. |
| 英文摘要 | To obtain Vibrio harveyi-resistant Litopenaeus vannamei shrimp used for study on immune response of shrimp avoid vibriosis, a three-round challenge selection procedure was applied. In this procedure, resistant shrimp were selected gradually via three rounds challenge experiment with a pathogenic strain of V harveyi at a median and controllable lethal dose of 96-h LD50 (the median lethal dose). After this procedure, the cumulative mortality of selected shrimp during 96 h after injection of V. harveyi at 2.0 x 10(6) cfu shrimp(-1) significantly decreased from 93.3% to 26.7%, the hours of beginning of death and the hours of attaining of the maximum cumulative mortality of shrimp prolonged from 4 h and 10 h to 8 h and 24 h, respectively. The LD50 of 6 h, 12 h, 24 h, 48 h and 96 h of selected shrimp significantly increased to 1.4 +/- 0.1 x 10(7) (p < 0.01), 5.5 +/- 0.4 x 10(6) (p < 0.01), 3.1 +/- 0.2 x 10(6) (p < 0.01), 2.7 +/- 0.1 x 10(6) (p < 0.01) and 2.7 +/- 0.1 x 10(6) cfu shrimp(-1) (p < 0.01), about 15.9, 15.3, 9.4, 10.0 and 10.4 times of that of normal shrimp, respectively. In conclusion, the resistance of shrimp to Vibrio significantly increased after the three-round challenge selection procedure. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | WHITE SPOT SYNDROME ; IMMUNE-RESPONSE ; GENE-EXPRESSION ; SYNDROME VIRUS ; SHRIMP ; SUSCEPTIBILITY ; ALGINOLYTICUS ; INFECTION |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.fsi.2013.04.033 |
| 语种 | 英语 |
| WOS记录号 | WOS:000322207500015 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16628]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China 3.Nanjiang Marine Biotechnol Co Ltd, Sanya 572000, Hainan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Hai-Hong,Liu, Xiao-Lin,Xiang, Jian-Hai,et al. Selection of Vibrio harveyi-resistant Litopenaeus vannamei via a three-round challenge selection with a pathogenic strain of V. harveyi[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(2):328-333. |
| APA | Huang, Hai-Hong,Liu, Xiao-Lin,Xiang, Jian-Hai,&Wang, Ping.(2013).Selection of Vibrio harveyi-resistant Litopenaeus vannamei via a three-round challenge selection with a pathogenic strain of V. harveyi.FISH & SHELLFISH IMMUNOLOGY,35(2),328-333. |
| MLA | Huang, Hai-Hong,et al."Selection of Vibrio harveyi-resistant Litopenaeus vannamei via a three-round challenge selection with a pathogenic strain of V. harveyi".FISH & SHELLFISH IMMUNOLOGY 35.2(2013):328-333. |
入库方式： OAI收割
来源：海洋研究所
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