Shrimp MyD88 responsive to bacteria and white spot syndrome virus
文献类型：期刊论文
| 作者 | Wen, Rong1,2; Li, Fuhua1; Sun, Zheng3; Li, Shihao1; Xiang, Jianhai1
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2013-02-01 |
| 卷号 | 34期号:2页码:574-581 |
| 关键词 | MyD88 Expression Immunity Penaeid shrimp |
| ISSN号 | 1050-4648 |
| 通讯作者 | Li, FH |
| 中文摘要 | The myeloid differentiation factor 88 (MyD88) is an important adapter protein which links members of the toll-like receptor (TLR) to the downstream components to activate related signaling pathways. In the present study, a MyD88 homolog (FcMyD88) was cloned from penaeid shrimp Fenneropenaeus chinensis. The ORF of FcMyD88 consisted of 1434 bp encoding a polypeptide of 477 amino acids which contains a death domain (DD) and a typical TLR and interleukin-1 receptor (IL-1R)-related (TIR) domain. Homology analysis revealed that the predicted amino acid (aa) sequence of FcMyD88 shared high similarities with a variety of previously reported MyD88s. The time-dependent expression patterns of FcMyD88 in cephalothoraxes of shrimp injected with Vibrio anguillarum (Gram-negative bacteria, G(-)), Micrococcus lysodeikticu (Gram-positive bacteria, G(+)) and white syndrome spot virus (WSSV) were analyzed at transcription and protein level by real-time PCR and western blotting, respectively. The expression level of FcMyD88 mRNA was significantly up-regulated at one hour (h), 12 h and 24 h after stimulation with both V anguillarum and M. lysodeikticu. The expression level of FcMyD88 protein was 2-fold up-regulated at 12 h post injection (hpi) of inactivated V. anguillarum while it didn't change after M. lysodeikticu injection during this period. After WSSV injection, the expression level of FcMyD88 mRNA remained relatively constant, while the FcMyD88 protein was significantly up-regulated at 12 and 24 hpi. These results suggested that the MyD88-dependent signaling pathway could be involved in the defense of both bacteria and WSSV infection. (C) 2012 Elsevier Ltd. All rights reserved. |
| 英文摘要 | The myeloid differentiation factor 88 (MyD88) is an important adapter protein which links members of the toll-like receptor (TLR) to the downstream components to activate related signaling pathways. In the present study, a MyD88 homolog (FcMyD88) was cloned from penaeid shrimp Fenneropenaeus chinensis. The ORF of FcMyD88 consisted of 1434 bp encoding a polypeptide of 477 amino acids which contains a death domain (DD) and a typical TLR and interleukin-1 receptor (IL-1R)-related (TIR) domain. Homology analysis revealed that the predicted amino acid (aa) sequence of FcMyD88 shared high similarities with a variety of previously reported MyD88s. The time-dependent expression patterns of FcMyD88 in cephalothoraxes of shrimp injected with Vibrio anguillarum (Gram-negative bacteria, G(-)), Micrococcus lysodeikticu (Gram-positive bacteria, G(+)) and white syndrome spot virus (WSSV) were analyzed at transcription and protein level by real-time PCR and western blotting, respectively. The expression level of FcMyD88 mRNA was significantly up-regulated at one hour (h), 12 h and 24 h after stimulation with both V anguillarum and M. lysodeikticu. The expression level of FcMyD88 protein was 2-fold up-regulated at 12 h post injection (hpi) of inactivated V. anguillarum while it didn't change after M. lysodeikticu injection during this period. After WSSV injection, the expression level of FcMyD88 mRNA remained relatively constant, while the FcMyD88 protein was significantly up-regulated at 12 and 24 hpi. These results suggested that the MyD88-dependent signaling pathway could be involved in the defense of both bacteria and WSSV infection. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | TOLL RECEPTOR ; FENNEROPENAEUS-CHINENSIS ; SIGNALING PATHWAY ; CELL-DEVELOPMENT ; DENDRITIC CELLS ; WSSV INFECTION ; TLR ; EXPRESSION ; DROSOPHILA ; FAMILY |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.fsi.2012.11.034 |
| 语种 | 英语 |
| WOS记录号 | WOS:000315076100019 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16631]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen, Rong,Li, Fuhua,Sun, Zheng,et al. Shrimp MyD88 responsive to bacteria and white spot syndrome virus[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(2):574-581. |
| APA | Wen, Rong,Li, Fuhua,Sun, Zheng,Li, Shihao,&Xiang, Jianhai.(2013).Shrimp MyD88 responsive to bacteria and white spot syndrome virus.FISH & SHELLFISH IMMUNOLOGY,34(2),574-581. |
| MLA | Wen, Rong,et al."Shrimp MyD88 responsive to bacteria and white spot syndrome virus".FISH & SHELLFISH IMMUNOLOGY 34.2(2013):574-581. |
入库方式： OAI收割
来源：海洋研究所
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