中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Spermatozoid life-span of two brown seaweeds, Saccharina japonica and Undaria pinnatifida, as measured by fertilization efficiency

文献类型:期刊论文

作者Li Jing1,2; Pang Shaojun1; Liu Feng1; Shan Tifeng1; Gao Suqin1
刊名CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
出版日期2013-07-01
卷号31期号:4页码:774-781
关键词gametogenesis life-span of sperm Saccharina japonica seaweeds Undaria pinnatifida
ISSN号0254-4059
通讯作者Pang, SJ
中文摘要During sexual reproduction of seaweeds, spermatozoid (sperm) discharge is triggered by chemical messengers (pheromones) released by the female gametes. The chemotactic ability of the sperm ensures fertilization success. Using unialgal male and female gametophyte material under designated standard gametogenesis testing (SGT) conditions, the potential life-span of the sperm of two seaweeds, Saccharina japonica and Undaria pinnatifida, was assessed by their ability to fertilize eggs. Results show that within 20-30 min after being discharged, sperm of both species could complete fertilization without an apparent decline in fertilization rate. Although fertilization rate 60-120 min after sperm discharge dropped significantly in both species, some sperm were viable enough to fertilize the eggs. In S. japonica, at 12A degrees C, some sperm were able to fertilize eggs up to 12 h after discharge. In both species, egg discharge rates (EDR) in the male and female mixed positive controls were significantly higher than those of all the sperm-testing groups. Doubling the seeded male gametophytes of S. japonica in the SGT tests significantly increased the EDR, further confirming the effect of the presence of the male on the female in terms of facilitating egg discharge from oogonia.
英文摘要During sexual reproduction of seaweeds, spermatozoid (sperm) discharge is triggered by chemical messengers (pheromones) released by the female gametes. The chemotactic ability of the sperm ensures fertilization success. Using unialgal male and female gametophyte material under designated standard gametogenesis testing (SGT) conditions, the potential life-span of the sperm of two seaweeds, Saccharina japonica and Undaria pinnatifida, was assessed by their ability to fertilize eggs. Results show that within 20-30 min after being discharged, sperm of both species could complete fertilization without an apparent decline in fertilization rate. Although fertilization rate 60-120 min after sperm discharge dropped significantly in both species, some sperm were viable enough to fertilize the eggs. In S. japonica, at 12A degrees C, some sperm were able to fertilize eggs up to 12 h after discharge. In both species, egg discharge rates (EDR) in the male and female mixed positive controls were significantly higher than those of all the sperm-testing groups. Doubling the seeded male gametophytes of S. japonica in the SGT tests significantly increased the EDR, further confirming the effect of the presence of the male on the female in terms of facilitating egg discharge from oogonia.
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
学科主题Marine & Freshwater Biology ; Oceanography
类目[WOS]Limnology ; Oceanography
研究领域[WOS]Marine & Freshwater Biology ; Oceanography
关键词[WOS]POLLEN ; PHAEOPHYCEAE ; POLLINATION ; LAMINARIALES ; SPOROPHYTES ; SEAGRASSES ; INDUCTION ; CULTURE ; RELEASE
收录类别SCI
原文出处10.1007/s00343-013-2207-y
语种英语
WOS记录号WOS:000321629400007
公开日期2014-07-17
源URL[http://ir.qdio.ac.cn/handle/337002/16635]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Li Jing,Pang Shaojun,Liu Feng,et al. Spermatozoid life-span of two brown seaweeds, Saccharina japonica and Undaria pinnatifida, as measured by fertilization efficiency[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(4):774-781.
APA Li Jing,Pang Shaojun,Liu Feng,Shan Tifeng,&Gao Suqin.(2013).Spermatozoid life-span of two brown seaweeds, Saccharina japonica and Undaria pinnatifida, as measured by fertilization efficiency.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(4),774-781.
MLA Li Jing,et al."Spermatozoid life-span of two brown seaweeds, Saccharina japonica and Undaria pinnatifida, as measured by fertilization efficiency".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.4(2013):774-781.

入库方式: OAI收割

来源:海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。