The increased transcriptional response and translocation of a Rel/NF-kappa B homologue in scallop Chlamys farreri during the immune stimulation
文献类型：期刊论文
| 作者 | Zhou, Zhi1; Wang, Mengqiang1 ; Zhao, Jianmin1; Wang, Lingling1; Gao, Yang1,2; Zhang, Huan1; Liu, Rui1; Song, Linsheng1
|
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 34期号:5页码:1209-1215 |
| ISSN号 | 1050-4648 |
| 关键词 | Rel NF-kappa B Transcription activity Immunomodulation Scallop |
| 通讯作者 | Wang, LL |
| 中文摘要 | The Rel/NF-kappa B transcription factors can function as key regulators to modulate the expression of immune-related genes in response to immune challenge or environmental stress. In the present study, a gene coding Rel/NF-kappa B homologue was identified from scallop Chlamys farreri (designated CfRel). Its deduced protein comprised 359 amino acids, and contained a conserved N-terminal Rel homology domain (RHO) and an IPT domain. There was an NF-kappa B/Rel/dorsal domain signature sequence in the RHD domain. The mRNA transcripts of CfRel could be detected in all the tested tissues including adductor muscle, mantle, gill, gonad, haemocytes, kidney and hepatopancreas, with the highest expression level in hepatopancreas. After LPS stimulation, there were two peaks of CfRel mRNA expression level in haemocytes at 6 h (25.25-fold, P < 0.05) and 24 h (59.66-fold, P < 0.05) respectively, while the mRNA expression of CfRel was only up-regulated at 3 h after PGN stimulation (2.35-fold, P < 0.05). By Western blotting technique, CfRel protein was observed in the cytoplasm and nucleus of scallop haemocytes, and its concentration in the haemocyte nucleus increased significantly at 3 h and 12 h after LPS stimulation. The noticeable NF-kappa B transcription activity of CfRel protein was determined by NF-kappa B luciferase reporter assays (122.43%, P < 0.05), and it decreased significantly (17.61%, P < 0.05) after the coexpression of scallop I kappa B protein. These results collectively suggested that CfRel mRNA transcripts and protein were induced by immune stimulation, and CfRel protein could extricate itself from I kappa B protein and transfer into the haemocyte nucleus to modulate the immune response in scallop. (C) 2013 Elsevier Ltd. All rights reserved. |
| 英文摘要 | The Rel/NF-kappa B transcription factors can function as key regulators to modulate the expression of immune-related genes in response to immune challenge or environmental stress. In the present study, a gene coding Rel/NF-kappa B homologue was identified from scallop Chlamys farreri (designated CfRel). Its deduced protein comprised 359 amino acids, and contained a conserved N-terminal Rel homology domain (RHO) and an IPT domain. There was an NF-kappa B/Rel/dorsal domain signature sequence in the RHD domain. The mRNA transcripts of CfRel could be detected in all the tested tissues including adductor muscle, mantle, gill, gonad, haemocytes, kidney and hepatopancreas, with the highest expression level in hepatopancreas. After LPS stimulation, there were two peaks of CfRel mRNA expression level in haemocytes at 6 h (25.25-fold, P < 0.05) and 24 h (59.66-fold, P < 0.05) respectively, while the mRNA expression of CfRel was only up-regulated at 3 h after PGN stimulation (2.35-fold, P < 0.05). By Western blotting technique, CfRel protein was observed in the cytoplasm and nucleus of scallop haemocytes, and its concentration in the haemocyte nucleus increased significantly at 3 h and 12 h after LPS stimulation. The noticeable NF-kappa B transcription activity of CfRel protein was determined by NF-kappa B luciferase reporter assays (122.43%, P < 0.05), and it decreased significantly (17.61%, P < 0.05) after the coexpression of scallop I kappa B protein. These results collectively suggested that CfRel mRNA transcripts and protein were induced by immune stimulation, and CfRel protein could extricate itself from I kappa B protein and transfer into the haemocyte nucleus to modulate the immune response in scallop. (C) 2013 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | C-TYPE LECTIN ; DROSOPHILA-MELANOGASTER ; SIGNALING PATHWAYS ; CRASSOSTREA-GIGAS ; PACIFIC OYSTER ; KINASE COMPLEX ; ACTIVATION ; HEMOCYTES ; PROTEIN ; EXPRESSION |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.fsi.2013.01.009 |
| 语种 | 英语 |
| WOS记录号 | WOS:000317870600023 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16649]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Zhi,Wang, Mengqiang,Zhao, Jianmin,et al. The increased transcriptional response and translocation of a Rel/NF-kappa B homologue in scallop Chlamys farreri during the immune stimulation[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1209-1215. |
| APA | Zhou, Zhi.,Wang, Mengqiang.,Zhao, Jianmin.,Wang, Lingling.,Gao, Yang.,...&Song, Linsheng.(2013).The increased transcriptional response and translocation of a Rel/NF-kappa B homologue in scallop Chlamys farreri during the immune stimulation.FISH & SHELLFISH IMMUNOLOGY,34(5),1209-1215. |
| MLA | Zhou, Zhi,et al."The increased transcriptional response and translocation of a Rel/NF-kappa B homologue in scallop Chlamys farreri during the immune stimulation".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1209-1215. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。