The megalocytivirus-induced protein CsMig1 enhances Cynoglossus semilaevis resistance against viral infection
文献类型：期刊论文
| 作者 | Wang, Wei1,2; Xiao, Zhi-zhong1; Sun, Li1 |
| 刊名 | VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
 |
| 出版日期 | 2013-01-15 |
| 卷号 | 151期号:1-2页码:173-179 |
| 关键词 | Cynoglossus semilaevis Megalocytivirus Interferon Antiviral |
| ISSN号 | 0165-2427 |
| 通讯作者 | Sun, L |
| 中文摘要 | Half-smooth tongue sole (Cynoglossus semilaevis) is an important economic fish species cultured in northern China. In this study, we identified and analyzed the expression and function of a megalocytivirus-induced gene, CsMig1, from tongue sole. The deduced amino acid sequence of CsMig1 is composed of 507 residues and contains no conserved domains. Blast analysis identified no close homologues of CsMig1. CsMig1 shares moderate sequence similarities in the N-terminal region with the Gig1 (i.e., grass carp hemorrhagic virus-induced gene) homologues of several teleost species. Quantitative real time RTPCR analysis showed that constitutive CsMig1 expression occurred, in increasing order, in heart, spleen, muscle, kidney, liver, gill, and gut. Experimental infection with the viral pathogen megalocytivirus upregulated CsMig1 expression in kidney, spleen, and liver in time-dependent manners. Treatment of head kidney lymphocytes with the culture supernatant of megalocytivirus-stimulated cells significantly enhanced CsMig1 expression. When head kidney lymphocytes were transfected with the Plasmid that constitutively expresses CsMig1, the cells exhibited significantly increased ability to resist megalocytivirus infection. Taken together, these results indicate that CsMig1 is a virus- and, possibly, interferon-induced novel immune factor that functions in the antiviral immunity of tongue sole. (C) 2012 Elsevier B.V. All rights reserved. |
| 英文摘要 | Half-smooth tongue sole (Cynoglossus semilaevis) is an important economic fish species cultured in northern China. In this study, we identified and analyzed the expression and function of a megalocytivirus-induced gene, CsMig1, from tongue sole. The deduced amino acid sequence of CsMig1 is composed of 507 residues and contains no conserved domains. Blast analysis identified no close homologues of CsMig1. CsMig1 shares moderate sequence similarities in the N-terminal region with the Gig1 (i.e., grass carp hemorrhagic virus-induced gene) homologues of several teleost species. Quantitative real time RTPCR analysis showed that constitutive CsMig1 expression occurred, in increasing order, in heart, spleen, muscle, kidney, liver, gill, and gut. Experimental infection with the viral pathogen megalocytivirus upregulated CsMig1 expression in kidney, spleen, and liver in time-dependent manners. Treatment of head kidney lymphocytes with the culture supernatant of megalocytivirus-stimulated cells significantly enhanced CsMig1 expression. When head kidney lymphocytes were transfected with the Plasmid that constitutively expresses CsMig1, the cells exhibited significantly increased ability to resist megalocytivirus infection. Taken together, these results indicate that CsMig1 is a virus- and, possibly, interferon-induced novel immune factor that functions in the antiviral immunity of tongue sole. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology ; Veterinary Sciences |
| 类目[WOS] | Immunology ; Veterinary Sciences |
| 研究领域[WOS] | Immunology ; Veterinary Sciences |
| 关键词[WOS] | PANCREATIC NECROSIS VIRUS ; DOUBLE-STRANDED-RNA ; PARALICHTHYS-OLIVACEUS ; TELEOST FISH ; ANTIVIRAL RESPONSES ; BIOLOGICAL-ACTIVITY ; JAPANESE FLOUNDER ; I INTERFERON ; EXPRESSION ; GENES |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.vetimm.2012.11.005 |
| 语种 | 英语 |
| WOS记录号 | WOS:000314012700019 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16652]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Wei,Xiao, Zhi-zhong,Sun, Li. The megalocytivirus-induced protein CsMig1 enhances Cynoglossus semilaevis resistance against viral infection[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2013,151(1-2):173-179. |
| APA | Wang, Wei,Xiao, Zhi-zhong,&Sun, Li.(2013).The megalocytivirus-induced protein CsMig1 enhances Cynoglossus semilaevis resistance against viral infection.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,151(1-2),173-179. |
| MLA | Wang, Wei,et al."The megalocytivirus-induced protein CsMig1 enhances Cynoglossus semilaevis resistance against viral infection".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 151.1-2(2013):173-179. |
入库方式： OAI收割
来源：海洋研究所
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