The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis
文献类型：期刊论文
| 作者 | Wang, M.1; Wang, L.1 ; Zhou, Z.1,2; Gao, Y.1,2; Wang, L.1,2; Shi, X.1,2; Gai, Y.1; Mu, C.1; Song, L.1; Wang, L
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| 刊名 | INTERNATIONAL JOURNAL OF IMMUNOGENETICS
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| 出版日期 | 2013-06-01 |
| 卷号 | 40期号:3页码:230-240 |
| ISSN号 | 1744-3121 |
| 通讯作者 | Wang, L |
| 中文摘要 | Catalase (CAT) is an antioxidant enzyme and plays a significant role in the protection against oxidative stress by reducing hydrogen peroxide. The CAT cDNA of Eriocheir sinensis (EsCAT) was cloned via RACE technique. The complete sequence of EsCAT cDNA consisted of a 5 untranslated regions (UTR) of 224bp, a 3 UTR of 1287bp with a poly (A) tail and an open reading frame (ORF) of 1542bp, which encoded a polypeptide of 513 amino acid residues with a calculated molecular mass of approximately 58.86kDa and a theoretical isoelectric point of 6.880. The deduced amino acid sequence of EsCAT contained a highly conserved proximal active-site signature motif (60FDRERIPERVVHAKGAL76) and a proximal hemeligand signature motif (350RLFSYNDTH358) and exhibited high similarity with other reported CATs. In the phylogenetic tree, EsCAT was clustered with the CATs from Scylla serrata and Portunus trituberculatus. The EsCAT transcripts were constitutively expressed in haepatopancreas, haemocytes, gill, gonad, muscle and heart, with highest expression level in haepatopancreas. The relative expression level of EsCAT mRNA in haemocytes was continuously up-regulated and reached the peak level at 48h post-Vibrio anguillarum challenge. The purified recombinant EsCAT protein displayed antioxidant activity against hydrogen peroxide with high thermal stability and broad spectrum of pH values. All these results demonstrated that EsCAT was an efficient antioxidant enzyme and potentially involved in the regulation of redox and innate immune response of crabs. |
| 英文摘要 | Catalase (CAT) is an antioxidant enzyme and plays a significant role in the protection against oxidative stress by reducing hydrogen peroxide. The CAT cDNA of Eriocheir sinensis (EsCAT) was cloned via RACE technique. The complete sequence of EsCAT cDNA consisted of a 5 untranslated regions (UTR) of 224bp, a 3 UTR of 1287bp with a poly (A) tail and an open reading frame (ORF) of 1542bp, which encoded a polypeptide of 513 amino acid residues with a calculated molecular mass of approximately 58.86kDa and a theoretical isoelectric point of 6.880. The deduced amino acid sequence of EsCAT contained a highly conserved proximal active-site signature motif (60FDRERIPERVVHAKGAL76) and a proximal hemeligand signature motif (350RLFSYNDTH358) and exhibited high similarity with other reported CATs. In the phylogenetic tree, EsCAT was clustered with the CATs from Scylla serrata and Portunus trituberculatus. The EsCAT transcripts were constitutively expressed in haepatopancreas, haemocytes, gill, gonad, muscle and heart, with highest expression level in haepatopancreas. The relative expression level of EsCAT mRNA in haemocytes was continuously up-regulated and reached the peak level at 48h post-Vibrio anguillarum challenge. The purified recombinant EsCAT protein displayed antioxidant activity against hydrogen peroxide with high thermal stability and broad spectrum of pH values. All these results demonstrated that EsCAT was an efficient antioxidant enzyme and potentially involved in the regulation of redox and innate immune response of crabs. |
| 学科主题 | Genetics & Heredity ; Immunology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Genetics & Heredity ; Immunology |
| 研究领域[WOS] | Genetics & Heredity ; Immunology |
| 关键词[WOS] | BEEF-LIVER CATALASE ; REACTIVE OXYGEN ; INNATE IMMUNITY ; SACCHAROMYCES-CEREVISIAE ; ANTIOXIDANT ENZYMES ; OLD ENZYME ; GENE ; CLONING ; EXPRESSION ; DAMAGE |
| 收录类别 | SCI |
| 原文出处 | 10.1111/iji.12019 |
| 语种 | 英语 |
| WOS记录号 | WOS:000318810200010 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16653]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Wang, L |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, M.,Wang, L.,Zhou, Z.,et al. The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis[J]. INTERNATIONAL JOURNAL OF IMMUNOGENETICS,2013,40(3):230-240. |
| APA | Wang, M..,Wang, L..,Zhou, Z..,Gao, Y..,Wang, L..,...&Wang, L.(2013).The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis.INTERNATIONAL JOURNAL OF IMMUNOGENETICS,40(3),230-240. |
| MLA | Wang, M.,et al."The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis".INTERNATIONAL JOURNAL OF IMMUNOGENETICS 40.3(2013):230-240. |
入库方式： OAI收割
来源：海洋研究所
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