Three vibrio-resistance related EST-SSR markers revealed by selective genotyping in the clam Meretrix meretrix
文献类型：期刊论文
| 作者 | Nie, Qing1,2 ; Yue, Xin1; Chai, Xueliang3; Wang, Hongxia1; Liu, Baozhong1; Liu, BZ
|
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2013-08-01 |
| 卷号 | 35期号:2页码:421-428 |
| 关键词 | Meretrix meretrix EST-SSR Marker-trait association Vibrio-resistance |
| ISSN号 | 1050-4648 |
| 通讯作者 | Liu, BZ |
| 中文摘要 | The clam Meretrix meretrix is an important commercial bivalve distributed in the coastal areas of South and Southeast Asia. In this study, marker trait association analyses were performed based on the stock materials of M. meretrix with different vibrio-resistance profile obtained by selective breeding. Forty-eight EST-SSR markers were screened and 27 polymorphic SSRs of them were genotyped in the clam stocks with different resistance to Vibrio parahaemolyticus (11-R and 11-S) and to Vibrio harveyi (09-R and 09-C). Allele frequency distributions of the SSRs among different stocks were compared using Pearson's Chi-square test, and three functional EST-SSR markers (MM959, MM4765 and MM8364) were found to be associated with vibrio-resistance trait. The 140-bp allele of MM959 and 128-bp allele of MM4765 had significantly higher frequencies in resistant groups (11-R and 09-R) than in susceptive/control groups (11-S and 09-C) (P < 0.01), which suggested that the clams carrying these two alleles have stronger resistance against vibrio. Clam individuals of 11-S were divided into three subgroups based on the survival time post-challenge, and the multi-dimensional scaling (MDS) analysis showed that clusters generated by genetic similarity revealed by the three SSR markers were consistent with the three subgroups distinctions. The putative functions of contig959, contig4765 and contig8364 also suggested that the three SSR-involved genes might play important roles in immunity of M. meretrix. All these results supported that EST-SSR markers MM959, MM4765 and MM8364 were associated with vibrio-resistance and would be useful for marker-assisted selection (MAS) in M. meretrix genetic breeding. (C) 2013 Elsevier Ltd. All rights reserved. |
| 英文摘要 | The clam Meretrix meretrix is an important commercial bivalve distributed in the coastal areas of South and Southeast Asia. In this study, marker trait association analyses were performed based on the stock materials of M. meretrix with different vibrio-resistance profile obtained by selective breeding. Forty-eight EST-SSR markers were screened and 27 polymorphic SSRs of them were genotyped in the clam stocks with different resistance to Vibrio parahaemolyticus (11-R and 11-S) and to Vibrio harveyi (09-R and 09-C). Allele frequency distributions of the SSRs among different stocks were compared using Pearson's Chi-square test, and three functional EST-SSR markers (MM959, MM4765 and MM8364) were found to be associated with vibrio-resistance trait. The 140-bp allele of MM959 and 128-bp allele of MM4765 had significantly higher frequencies in resistant groups (11-R and 09-R) than in susceptive/control groups (11-S and 09-C) (P < 0.01), which suggested that the clams carrying these two alleles have stronger resistance against vibrio. Clam individuals of 11-S were divided into three subgroups based on the survival time post-challenge, and the multi-dimensional scaling (MDS) analysis showed that clusters generated by genetic similarity revealed by the three SSR markers were consistent with the three subgroups distinctions. The putative functions of contig959, contig4765 and contig8364 also suggested that the three SSR-involved genes might play important roles in immunity of M. meretrix. All these results supported that EST-SSR markers MM959, MM4765 and MM8364 were associated with vibrio-resistance and would be useful for marker-assisted selection (MAS) in M. meretrix genetic breeding. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | QUANTITATIVE-TRAIT LOCI ; FLOUNDER PARALICHTHYS-OLIVACEUS ; GENETIC-MARKERS ; ASSISTED SELECTION ; MOLECULAR CHARACTERIZATION ; DISEASE ; LINKAGE ; GROWTH ; QTL ; ASSOCIATION |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.fsi.2013.04.046 |
| 语种 | 英语 |
| WOS记录号 | WOS:000322207500027 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16663]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, BZ |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Zhejiang Mariculture Res Inst, Wenzhou 325005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Nie, Qing,Yue, Xin,Chai, Xueliang,et al. Three vibrio-resistance related EST-SSR markers revealed by selective genotyping in the clam Meretrix meretrix[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(2):421-428. |
| APA | Nie, Qing,Yue, Xin,Chai, Xueliang,Wang, Hongxia,Liu, Baozhong,&Liu, BZ.(2013).Three vibrio-resistance related EST-SSR markers revealed by selective genotyping in the clam Meretrix meretrix.FISH & SHELLFISH IMMUNOLOGY,35(2),421-428. |
| MLA | Nie, Qing,et al."Three vibrio-resistance related EST-SSR markers revealed by selective genotyping in the clam Meretrix meretrix".FISH & SHELLFISH IMMUNOLOGY 35.2(2013):421-428. |
入库方式： OAI收割
来源：海洋研究所
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