Transcriptome Analysis of Artificial Hybrid Pufferfish Jiyan-1and Its Parental Species: Implications for Pufferfish Heterosis
文献类型：期刊论文
| 作者 | Gao, Yang1,2 ; Zhang, Huan1; Gao, Qiang1; Wang, Lingling1; Zhang, Fuchong3; Siva, Vinu S.1,2; Zhou, Zhi1; Song, Linsheng1; Zhang, Shicui4
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| 刊名 | PLOS ONE
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| 出版日期 | 2013-03-08 |
| 卷号 | 8期号:3页码:e58453 |
| ISSN号 | 1932-6203 |
| 通讯作者 | Zhang, FC |
| 中文摘要 | Jiyan-1 puffer, the F1 hybrid of Takifugu rubripes and Takifugu flavidus, displays obvious heterosis in the growth performance, flavor and stress tolerance. In the present study, comparative analysis for the transcriptomes of T. rubripes, T. flavidus and Jiyan-1 was performed aiming to reveal the possible mechanisms of heterosis in pufferfish. Whole transcriptomes were sequenced using the SOLiD4 platform, and a total of 44,305 transcripts corresponding to 18,164 genes were identified collectively. A total of 14,148 transcripts were differentially expressed. By comparing the gene expression patterns of the three samples, the coexistence of overdominance, dominance, underdominance and additivity was observed in the gene action modes of Jiyan-1. There were 2,237 transcripts in the intersection of the differentially expressed transcripts from Jiyan-1 versus T. rubripes and Jiyan-1 versus T. flavidus, among which 213 transcripts were also in the T. rubripes versus T. flavidus. The potential functions of the remaining 2,024 transcripts were mainly associated with metabolic process, nucleotide binding and catalytic activity. The enrichment results indicated metabolism was the most activated biological function in the heterosis. In addition, 35 KEGG pathways were retrieved as affiliated with more than three differentially expressed transcripts and 8,579 potentially novel transcript isoforms were identified for Jiyan-1. The present study revealed the coexistence of multiple gene actions in the hybrid puffer, indicated the importance of metabolism, ion binding function and kinase activity, as well as provided a list of candidate genes and pathways for heterosis. It could be helpful for the better understanding of the determination and regulation mechanisms of heterosis. |
| 英文摘要 | Jiyan-1 puffer, the F1 hybrid of Takifugu rubripes and Takifugu flavidus, displays obvious heterosis in the growth performance, flavor and stress tolerance. In the present study, comparative analysis for the transcriptomes of T. rubripes, T. flavidus and Jiyan-1 was performed aiming to reveal the possible mechanisms of heterosis in pufferfish. Whole transcriptomes were sequenced using the SOLiD4 platform, and a total of 44,305 transcripts corresponding to 18,164 genes were identified collectively. A total of 14,148 transcripts were differentially expressed. By comparing the gene expression patterns of the three samples, the coexistence of overdominance, dominance, underdominance and additivity was observed in the gene action modes of Jiyan-1. There were 2,237 transcripts in the intersection of the differentially expressed transcripts from Jiyan-1 versus T. rubripes and Jiyan-1 versus T. flavidus, among which 213 transcripts were also in the T. rubripes versus T. flavidus. The potential functions of the remaining 2,024 transcripts were mainly associated with metabolic process, nucleotide binding and catalytic activity. The enrichment results indicated metabolism was the most activated biological function in the heterosis. In addition, 35 KEGG pathways were retrieved as affiliated with more than three differentially expressed transcripts and 8,579 potentially novel transcript isoforms were identified for Jiyan-1. The present study revealed the coexistence of multiple gene actions in the hybrid puffer, indicated the importance of metabolism, ion binding function and kinase activity, as well as provided a list of candidate genes and pathways for heterosis. It could be helpful for the better understanding of the determination and regulation mechanisms of heterosis. |
| WOS标题词 | Science & Technology |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | ELITE RICE HYBRID ; GENE-EXPRESSION ; INSECTICIDE RESISTANCE ; ARABIDOPSIS-THALIANA ; FUNCTIONAL GENOMICS ; METABOLIC-ACTIVITY ; CYTOCHROMES P450 ; MAIZE ; DOMINANCE ; REVEALS |
| 收录类别 | SCI |
| 原文出处 | 10.1371/journal.pone.0058453 |
| 语种 | 英语 |
| WOS记录号 | WOS:000318679900070 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16665]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Ocean & Fishery Sci Res Inst Hebei Prov, Qinhuangdao, Peoples R China 4.Ocean Univ China, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Yang,Zhang, Huan,Gao, Qiang,et al. Transcriptome Analysis of Artificial Hybrid Pufferfish Jiyan-1and Its Parental Species: Implications for Pufferfish Heterosis[J]. PLOS ONE,2013,8(3):e58453. |
| APA | Gao, Yang.,Zhang, Huan.,Gao, Qiang.,Wang, Lingling.,Zhang, Fuchong.,...&Zhang, Shicui.(2013).Transcriptome Analysis of Artificial Hybrid Pufferfish Jiyan-1and Its Parental Species: Implications for Pufferfish Heterosis.PLOS ONE,8(3),e58453. |
| MLA | Gao, Yang,et al."Transcriptome Analysis of Artificial Hybrid Pufferfish Jiyan-1and Its Parental Species: Implications for Pufferfish Heterosis".PLOS ONE 8.3(2013):e58453. |
入库方式： OAI收割
来源：海洋研究所
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