中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Two Rab GTPases, EsRab-1 and EsRab-3, involved in anti-bacterial response of Chinese mitten crab Eriocheir sinensis

文献类型:期刊论文

作者Wang, Leilei1,2; Li, Ling1; Wang, Lingling1; Yang, Jialong1,2; Wang, Jingjing1,2; Zhou, Zhi1; Zhang, Huan1; Song, Linsheng1
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2013-09-01
卷号35期号:3页码:1007-1015
关键词Rab GTPase Eriocheir sinensis Anti-bacterial immunity Innate immunity
ISSN号1050-4648
通讯作者Song, LS
中文摘要Rab GTPase is essential for the control of intracellular membrane trafficking in all eukaryotic cells and further affects the ability of phagocytic cells to scavenge pathogen. In the present study, the cDNAs for two crab Rab proteins (EsRab-1 and EsRab-3) were identified from the Chinese mitten crab Eriocheir sinensis by rapid amplification of cDNA ends (RACE) approaches and expressed sequence tag (EST) analysis. The full-length cDNAs of EsRab-1 and EsRab-3 were of 892 bp and 965 bp with ORFs of 615 bp and 630 bp, respectively. The cDNAs encoded two peptides of 204 and 209 amino acid residues with the conserved GTP/Mg2+ binding sites, Switch I region and Switch II region in RAB domains. The mRNA transcripts of EsRab-1 and EsRab-3 were both highest expressed in hepatopancreas, and marginally expressed in other tissues including hemocytes, muscle, gonad, gill and heart. After the crabs were challenged by bacteria Vibrio anguillarum, the expression levels of both EsRab-1 and EsRab-3 in hemocytes were significantly up-regulated, and reached the highest level at 1.5 h post-stimulation, which was 7-fold (P < 0.05) and 6-fold (P < 0.01) of blank group for EsRab-1 and EsRab-3, respectively. No significant change of mRNA expression was detected for either EsRab-1 or EsRab-3 in crabs stimulated by Pichia pastoris. These results clearly suggested the involvement of Rab proteins in crab anti-bacterial immunity. (C) 2013 Elsevier Ltd. All rights reserved.
英文摘要Rab GTPase is essential for the control of intracellular membrane trafficking in all eukaryotic cells and further affects the ability of phagocytic cells to scavenge pathogen. In the present study, the cDNAs for two crab Rab proteins (EsRab-1 and EsRab-3) were identified from the Chinese mitten crab Eriocheir sinensis by rapid amplification of cDNA ends (RACE) approaches and expressed sequence tag (EST) analysis. The full-length cDNAs of EsRab-1 and EsRab-3 were of 892 bp and 965 bp with ORFs of 615 bp and 630 bp, respectively. The cDNAs encoded two peptides of 204 and 209 amino acid residues with the conserved GTP/Mg2+ binding sites, Switch I region and Switch II region in RAB domains. The mRNA transcripts of EsRab-1 and EsRab-3 were both highest expressed in hepatopancreas, and marginally expressed in other tissues including hemocytes, muscle, gonad, gill and heart. After the crabs were challenged by bacteria Vibrio anguillarum, the expression levels of both EsRab-1 and EsRab-3 in hemocytes were significantly up-regulated, and reached the highest level at 1.5 h post-stimulation, which was 7-fold (P < 0.05) and 6-fold (P < 0.01) of blank group for EsRab-1 and EsRab-3, respectively. No significant change of mRNA expression was detected for either EsRab-1 or EsRab-3 in crabs stimulated by Pichia pastoris. These results clearly suggested the involvement of Rab proteins in crab anti-bacterial immunity. (C) 2013 Elsevier Ltd. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科主题Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
类目[WOS]Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]ANTILIPOPOLYSACCHARIDE FACTOR ; PHAGOSOME MATURATION ; VIRUS-INFECTION ; TREMOR DISEASE ; PROTEINS ; SHRIMP ; EXPRESSION ; PHAGOCYTOSIS ; CLONING ; GENE
收录类别SCI
原文出处10.1016/j.fsi.2013.06.010
语种英语
WOS记录号WOS:000324511700047
公开日期2014-07-17
源URL[http://ir.qdio.ac.cn/handle/337002/16673]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Wang, Leilei,Li, Ling,Wang, Lingling,et al. Two Rab GTPases, EsRab-1 and EsRab-3, involved in anti-bacterial response of Chinese mitten crab Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(3):1007-1015.
APA Wang, Leilei.,Li, Ling.,Wang, Lingling.,Yang, Jialong.,Wang, Jingjing.,...&Song, Linsheng.(2013).Two Rab GTPases, EsRab-1 and EsRab-3, involved in anti-bacterial response of Chinese mitten crab Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,35(3),1007-1015.
MLA Wang, Leilei,et al."Two Rab GTPases, EsRab-1 and EsRab-3, involved in anti-bacterial response of Chinese mitten crab Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 35.3(2013):1007-1015.

入库方式: OAI收割

来源:海洋研究所

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