Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis
文献类型：期刊论文
| 作者 | Wu, Xiaojie1; Huang, Aiyou2,3; Xu, Meiling1; Wang, Chao2,3; Jia, Zhaojun1; Wang, Guangce1,2; Niu, Jianfeng2 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013-04-18 |
| 卷号 | 8期号:4页码:e60740 |
| ISSN号 | 1932-6203 |
| 通讯作者 | Wang, GC |
| 中文摘要 | In order to identify the optimal internal control for relative real-time PCR when studying target gene expression in the red alga Porphyra yezoensis, we quantified the expression of seven housekeeping genes (18S ribosomal RNA, 30S ribosomal protein S8, Polyubiquitin-2, Glyceraldehyde-3-phosphate dehydrogenase, Elongation factor 1-alpha, Beta-tubulin and Actin 3) at different life-history stages. Absolute quantification was done by normalization to total RNA quantity and by normalization to genomic DNA quantity. We used these two normalization approaches, comparing the differences of expression levels of all candidate housekeeping genes between any two generations and across three life-history stages (filamentous sporophytes, leafy gametophytes and conchospores). We found GAPDH had the best stability in all cases and we recommend that GAPDH be considered as a potential internal control for gene expression studies at different life-history stages in P. yezoensis. |
| 英文摘要 | In order to identify the optimal internal control for relative real-time PCR when studying target gene expression in the red alga Porphyra yezoensis, we quantified the expression of seven housekeeping genes (18S ribosomal RNA, 30S ribosomal protein S8, Polyubiquitin-2, Glyceraldehyde-3-phosphate dehydrogenase, Elongation factor 1-alpha, Beta-tubulin and Actin 3) at different life-history stages. Absolute quantification was done by normalization to total RNA quantity and by normalization to genomic DNA quantity. We used these two normalization approaches, comparing the differences of expression levels of all candidate housekeeping genes between any two generations and across three life-history stages (filamentous sporophytes, leafy gametophytes and conchospores). We found GAPDH had the best stability in all cases and we recommend that GAPDH be considered as a potential internal control for gene expression studies at different life-history stages in P. yezoensis. |
| WOS标题词 | Science & Technology |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | REAL-TIME PCR ; POLYMERASE-CHAIN-REACTION ; RT-PCR ; MESSENGER-RNA ; RIBOSOMAL-RNA ; NORMALIZATION ; RHODOPHYTA ; QUANTIFICATION ; VALIDATION ; BANGIALES |
| 收录类别 | SCI |
| 原文出处 | 10.1371/journal.pone.0060740 |
| 语种 | 英语 |
| WOS记录号 | WOS:000317908700006 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16675]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Xiaojie,Huang, Aiyou,Xu, Meiling,et al. Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis[J]. PLOS ONE,2013,8(4):e60740. |
| APA | Wu, Xiaojie.,Huang, Aiyou.,Xu, Meiling.,Wang, Chao.,Jia, Zhaojun.,...&Niu, Jianfeng.(2013).Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis.PLOS ONE,8(4),e60740. |
| MLA | Wu, Xiaojie,et al."Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis".PLOS ONE 8.4(2013):e60740. |
入库方式： OAI收割
来源：海洋研究所
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