Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum
文献类型：期刊论文
| 作者 | Liu Yun1,2; Song Xiuxian1 ; Han Xiaotian1; Yu Zhiming1; Yu, ZM
|
| 刊名 | ACTA OCEANOLOGICA SINICA
 |
| 出版日期 | 2013-06-01 |
| 卷号 | 32期号:6页码:82-88 |
| 关键词 | high-affinity nitrate transporter gene phosphate starvation nitrate concentrations real-time PCR Skeletonema costatum |
| ISSN号 | 0253-505X |
| 通讯作者 | Yu, ZM |
| 中文摘要 | To verify the feasibility of high-affinity nitrate transporter gene (Nrt2) as an indicator of nitrogen status, changes in the transcript levels of transcripts associated with phosphate starvation and different nitrate concentrations were studied using real-time quantitative reverse-transcription PCR (QRT-PCR) technology in batch cultures of Skeletonema costatum. The results show that compared with P-replete condition, P starvation could reduce the Nrt2 transcript levels apparently. Nrt2 transcript levels had a significant negative linear correlation with nitrate concentrations below 40 mu mol/L. The results of 48 h short-term incubation experiment under different nitrate concentrations confirmed this correlation, and the following regression equation is built: y = -3.305x + 98.95, R-2 = 0.988, where x represents nitrate concentrations (<40 mu mol/L) and y represents the Nrt2 transcript levels. |
| 英文摘要 | To verify the feasibility of high-affinity nitrate transporter gene (Nrt2) as an indicator of nitrogen status, changes in the transcript levels of transcripts associated with phosphate starvation and different nitrate concentrations were studied using real-time quantitative reverse-transcription PCR (QRT-PCR) technology in batch cultures of Skeletonema costatum. The results show that compared with P-replete condition, P starvation could reduce the Nrt2 transcript levels apparently. Nrt2 transcript levels had a significant negative linear correlation with nitrate concentrations below 40 mu mol/L. The results of 48 h short-term incubation experiment under different nitrate concentrations confirmed this correlation, and the following regression equation is built: y = -3.305x + 98.95, R-2 = 0.988, where x represents nitrate concentrations (<40 mu mol/L) and y represents the Nrt2 transcript levels. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | Oceanography |
| 类目[WOS] | Oceanography |
| 研究领域[WOS] | Oceanography |
| 关键词[WOS] | THALASSIOSIRA-PSEUDONANA ; MARINE-PHYTOPLANKTON ; NITROGEN DEFICIENCY ; EXPRESSION ; STRESS ; BACILLARIOPHYCEAE ; PRYMNESIOPHYCEAE ; ECOSYSTEMS ; METABOLISM ; CLONING |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s13131-013-0324-2 |
| 语种 | 英语 |
| WOS记录号 | WOS:000320094400011 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16478]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Yu, ZM |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu Yun,Song Xiuxian,Han Xiaotian,et al. Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum[J]. ACTA OCEANOLOGICA SINICA,2013,32(6):82-88. |
| APA | Liu Yun,Song Xiuxian,Han Xiaotian,Yu Zhiming,&Yu, ZM.(2013).Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum.ACTA OCEANOLOGICA SINICA,32(6),82-88. |
| MLA | Liu Yun,et al."Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum".ACTA OCEANOLOGICA SINICA 32.6(2013):82-88. |
入库方式： OAI收割
来源：海洋研究所
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