Microbial genetic diversity and ciliate community structure along an environmental gradient in coastal soil
文献类型：期刊论文
| 作者 | Zhao, Feng1,2; Xu, Kuidong1 ; Xu, KD
|
| 刊名 | EUROPEAN JOURNAL OF PROTISTOLOGY
 |
| 出版日期 | 2013-11-01 |
| 卷号 | 49期号:4页码:516-525 |
| 关键词 | Ciliate community Coastal soil Molecular diversity Salinisation |
| ISSN号 | 0932-4739 |
| 通讯作者 | Xu, KD |
| 中文摘要 | We investigated the microbial genetic diversity and ciliate community in coastal soil from five sites with an environmental gradient using denaturing gradient gel electrophoresis (DGGE), gene sequencing and the Ludox-QPS method. The analyses of both the 16S ribosomal RNA (rRNA) gene and 18S rRNA gene DGGE resulted in equal or even a higher number of bands found in the samples taken from the high-salinity sites IV and V than in those taken from the low-to-moderate-salinity sites I-III. Cluster analysis of both DGGE profiles classified the five sites into three main groups (sites I, II and III and IV and V), which corresponded well to the analysis of environmental factors. There were 13 species observed at site I, three species at site II and nine species at site III, while no active ciliates were observed at the high-salinity sites IV and V. By contrast, the ciliate-specific DGGE revealed a higher number of bands in the samples taken from the high-salinity soil. Furthermore, gene sequencing suggested that the ciliates in the high-salinity soil comprised forms originating not only from soil but also from marine environments. The data indicate that saline soil may maintain a high diversity of ciliates and soil salinity might be the most influential factor regulating the community structure of ciliates. (C) 2013 Elsevier GmbH. All rights reserved. |
| 英文摘要 | We investigated the microbial genetic diversity and ciliate community in coastal soil from five sites with an environmental gradient using denaturing gradient gel electrophoresis (DGGE), gene sequencing and the Ludox-QPS method. The analyses of both the 16S ribosomal RNA (rRNA) gene and 18S rRNA gene DGGE resulted in equal or even a higher number of bands found in the samples taken from the high-salinity sites IV and V than in those taken from the low-to-moderate-salinity sites I-III. Cluster analysis of both DGGE profiles classified the five sites into three main groups (sites I, II and III and IV and V), which corresponded well to the analysis of environmental factors. There were 13 species observed at site I, three species at site II and nine species at site III, while no active ciliates were observed at the high-salinity sites IV and V. By contrast, the ciliate-specific DGGE revealed a higher number of bands in the samples taken from the high-salinity soil. Furthermore, gene sequencing suggested that the ciliates in the high-salinity soil comprised forms originating not only from soil but also from marine environments. The data indicate that saline soil may maintain a high diversity of ciliates and soil salinity might be the most influential factor regulating the community structure of ciliates. (C) 2013 Elsevier GmbH. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Microbiology |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | 16S RIBOSOMAL-RNA ; SALINITY GRADIENT ; MOLECULAR TECHNIQUES ; BACTERIAL ; CHINA ; DGGE ; ASSEMBLAGES ; PATTERNS ; PROTOZOA ; RIVER |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.ejop.2013.01.002 |
| 语种 | 英语 |
| WOS记录号 | WOS:000326360200003 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16336]  |
| 专题 | 海洋研究所_海洋生物分类与系统演化实验室 |
| 通讯作者 | Xu, KD |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Feng,Xu, Kuidong,Xu, KD. Microbial genetic diversity and ciliate community structure along an environmental gradient in coastal soil[J]. EUROPEAN JOURNAL OF PROTISTOLOGY,2013,49(4):516-525. |
| APA | Zhao, Feng,Xu, Kuidong,&Xu, KD.(2013).Microbial genetic diversity and ciliate community structure along an environmental gradient in coastal soil.EUROPEAN JOURNAL OF PROTISTOLOGY,49(4),516-525. |
| MLA | Zhao, Feng,et al."Microbial genetic diversity and ciliate community structure along an environmental gradient in coastal soil".EUROPEAN JOURNAL OF PROTISTOLOGY 49.4(2013):516-525. |
入库方式： OAI收割
来源：海洋研究所
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