Spatio-temporal Variations in the Molecular Diversity of Microeukaryotes in Particular Ciliates in Soil of the Yellow River Delta, China
文献类型：期刊论文
| 作者 | Zhao, Feng1,2; Xu, Kuidong1 ; Zhang, Dechao1; Xu, KD
|
| 刊名 | JOURNAL OF EUKARYOTIC MICROBIOLOGY
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 60期号:3页码:282-290 |
| 关键词 | Ciliate community coastal wetland microeukaryotic diversity salinization seasonal variation |
| ISSN号 | 1066-5234 |
| 通讯作者 | Xu, KD |
| 中文摘要 | The Yellow River delta in China is one of the most active regions of landocean interaction. It has suffered serious salinization due to drying-up of the Yellow River, rising sea level, and seashore erosion, and thus represents a special and extreme environment. We evaluated the microeukaryotic molecular diversity and its response to change of seasons and environmental variables, in particular salinity in the soil of the Yellow River delta, by denaturing gradient gel electrophoresis (DGGE) and gene sequencing. The sequencing of the microeukaryotic DGGE bands revealed the presence of diverse groups dominated by protists in particular ciliates. We further recovered a high diversity of marine and soil ciliates inhabiting in coastal soil using the ciliate-specific DGGE. The neighbor-joining tree indicated that the ciliate 18S rDNA sequences from high-salinity soil were affiliated to Colpodea, Spirotrichea, Litostomatea, and Oligohymenophorea, while all the sequences unique to the low-salinity soil were affiliated to Colpodea. Statistical analysis indicated that the microeukaryotic molecular diversity was significantly different among sites, while statistically indistinguishable among seasons. Soil salinity might be the main factor regulating the distribution of microeukaryotes in the soil from the Yellow River delta. |
| 英文摘要 | The Yellow River delta in China is one of the most active regions of landocean interaction. It has suffered serious salinization due to drying-up of the Yellow River, rising sea level, and seashore erosion, and thus represents a special and extreme environment. We evaluated the microeukaryotic molecular diversity and its response to change of seasons and environmental variables, in particular salinity in the soil of the Yellow River delta, by denaturing gradient gel electrophoresis (DGGE) and gene sequencing. The sequencing of the microeukaryotic DGGE bands revealed the presence of diverse groups dominated by protists in particular ciliates. We further recovered a high diversity of marine and soil ciliates inhabiting in coastal soil using the ciliate-specific DGGE. The neighbor-joining tree indicated that the ciliate 18S rDNA sequences from high-salinity soil were affiliated to Colpodea, Spirotrichea, Litostomatea, and Oligohymenophorea, while all the sequences unique to the low-salinity soil were affiliated to Colpodea. Statistical analysis indicated that the microeukaryotic molecular diversity was significantly different among sites, while statistically indistinguishable among seasons. Soil salinity might be the main factor regulating the distribution of microeukaryotes in the soil from the Yellow River delta. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Microbiology |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | GRADIENT GEL-ELECTROPHORESIS ; EUKARYOTIC DIVERSITY ; COMMUNITY STRUCTURE ; ECOSYSTEM ; PATTERNS ; PROTOZOA ; DNA ; MIGRATION ; PROTISTS ; RECOVERY |
| 收录类别 | SCI |
| 原文出处 | 10.1111/jeu.12035 |
| 语种 | 英语 |
| WOS记录号 | WOS:000318245900006 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16343]  |
| 专题 | 海洋研究所_海洋生物分类与系统演化实验室 |
| 通讯作者 | Xu, KD |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Feng,Xu, Kuidong,Zhang, Dechao,et al. Spatio-temporal Variations in the Molecular Diversity of Microeukaryotes in Particular Ciliates in Soil of the Yellow River Delta, China[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2013,60(3):282-290. |
| APA | Zhao, Feng,Xu, Kuidong,Zhang, Dechao,&Xu, KD.(2013).Spatio-temporal Variations in the Molecular Diversity of Microeukaryotes in Particular Ciliates in Soil of the Yellow River Delta, China.JOURNAL OF EUKARYOTIC MICROBIOLOGY,60(3),282-290. |
| MLA | Zhao, Feng,et al."Spatio-temporal Variations in the Molecular Diversity of Microeukaryotes in Particular Ciliates in Soil of the Yellow River Delta, China".JOURNAL OF EUKARYOTIC MICROBIOLOGY 60.3(2013):282-290. |
入库方式： OAI收割
来源：海洋研究所
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