Genome and Transcriptome Analyses Provide Insight into the Euryhaline Adaptation Mechanism of Crassostrea gigas
文献类型：期刊论文
| 作者 | Meng, Jie1,2 ; Zhu, Qihui1,2; Zhang, Linlin1; Li, Chunyan1,2; Li, Li1; She, Zhicai1,2; Huang, Baoyu1,2; Zhang, Guofan1; Li, L
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| 刊名 | PLOS ONE
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| 出版日期 | 2013-03-12 |
| 卷号 | 8期号:3页码:e58563 |
| ISSN号 | 1932-6203 |
| 通讯作者 | Li, L |
| 中文摘要 | Background: The Pacific oyster, Crassostrea gigas, has developed special mechanisms to regulate its osmotic balance to adapt to fluctuations of salinities in coastal zones. To understand the oyster's euryhaline adaptation, we analyzed salt stress effectors metabolism pathways under different salinities (salt 5, 10, 15, 20, 25, 30 and 40 for 7 days) using transcriptome data, physiology experiment and quantitative real-time PCR. Results: Transcriptome data uncovered 189, 480, 207 and 80 marker genes for monitoring physiology status of oysters and the environment conditions. Three known salt stress effectors (involving ion channels, aquaporins and free amino acids) were examined. The analysis of ion channels and aquaporins indicated that 7 days long-term salt stress inhibited voltage-gated Na+/K+ channel and aquaporin but increased calcium-activated K+ channel and Ca2+ channel. As the most important category of osmotic stress effector, we analyzed the oyster FAAs metabolism pathways (including taurine, glycine, alanine, beta-alanine, proline and arginine) and explained FAAs functional mechanism for oyster low salinity adaptation. FAAs metabolism key enzyme genes displayed expression differentiation in low salinity adapted individuals comparing with control which further indicated that FAAs played important roles for oyster salinity adaptation. A global metabolic pathway analysis (iPath) of oyster expanded genes displayed a co-expansion of FAAs metabolism in C. gigas compared with seven other species, suggesting oyster's powerful ability regarding FAAs metabolism, allowing it to adapt to fluctuating salinities, which may be one important mechanism underlying euryhaline adaption in oyster. Additionally, using transcriptome data analysis, we uncovered salt stress transduction networks in C. gigas. Conclusions: Our results represented oyster salt stress effectors functional mechanisms under salt stress conditions and explained the expansion of FAAs metabolism pathways as the most important effectors for oyster euryhaline adaptation. This study was the first to explain oyster euryhaline adaptation at a genome-wide scale in C. gigas. |
| 英文摘要 | Background: The Pacific oyster, Crassostrea gigas, has developed special mechanisms to regulate its osmotic balance to adapt to fluctuations of salinities in coastal zones. To understand the oyster's euryhaline adaptation, we analyzed salt stress effectors metabolism pathways under different salinities (salt 5, 10, 15, 20, 25, 30 and 40 for 7 days) using transcriptome data, physiology experiment and quantitative real-time PCR. |
| WOS标题词 | Science & Technology |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | CELL-VOLUME REGULATION ; PACIFIC OYSTER ; AMINO-ACID ; INTERTIDAL ZONE ; GENE-EXPRESSION ; MARINE MOLLUSKS ; MARKER GENES ; SALINITY ; STRESS ; TAURINE |
| 收录类别 | SCI |
| 原文出处 | 10.1371/journal.pone.0058563 |
| 语种 | 英语 |
| WOS记录号 | WOS:000316252500030 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16701]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, L |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, Jie,Zhu, Qihui,Zhang, Linlin,et al. Genome and Transcriptome Analyses Provide Insight into the Euryhaline Adaptation Mechanism of Crassostrea gigas[J]. PLOS ONE,2013,8(3):e58563. |
| APA | Meng, Jie.,Zhu, Qihui.,Zhang, Linlin.,Li, Chunyan.,Li, Li.,...&Li, L.(2013).Genome and Transcriptome Analyses Provide Insight into the Euryhaline Adaptation Mechanism of Crassostrea gigas.PLOS ONE,8(3),e58563. |
| MLA | Meng, Jie,et al."Genome and Transcriptome Analyses Provide Insight into the Euryhaline Adaptation Mechanism of Crassostrea gigas".PLOS ONE 8.3(2013):e58563. |
入库方式： OAI收割
来源：海洋研究所
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