Germ line-specific and sexually dimorphic expression of a dead end gene homologue in turbot (Scophthalmus maximus)
文献类型：期刊论文
| 作者 | Lin, F.1,2; Zhao, C. Y.1,2; Xu, S. H.1; Ma, D. Y.1; Xiao, Z. Z.1; Xiao, Y. S.1; Xu, C. A.3; Liu, Q. H.1; Li, J.1 ; Li, J
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| 刊名 | THERIOGENOLOGY
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| 出版日期 | 2013-10-01 |
| 卷号 | 80期号:6页码:665-672 |
| ISSN号 | 0093-691X |
| 关键词 | Dead end Scophthalmus maximus Teleost Germ cell Gonadal development |
| 通讯作者 | Li, J |
| 中文摘要 | Germ cells are indispensable for gonadal development and fertility. However, the physiological mechanisms regulating germ cell development in marine fish are poorly understood due to a lack of germ cell markers. The dead end (dnd) gene is a vertebrate-specific component of germplasm crucial for primordial germ cells (PGCs) migration and development in teleosts. In this study, we identified a dnd homologue (Smdnd) in turbot (Scophthalmus maximus) and investigated its expression pattern during embryogenesis and gonadal development. The deduced amino acid sequence of Smdnd shared several conserved motifs of Dnd homologues as well as high identity to other Dnd proteins. Phylogenetic analysis revealed that the SmDnd was closely related to its teleost counterparts. Reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization revealed that Smdnd transcripts could be exclusively detected in germ cells, including presumptive PGC and adult male and female germ cells. In addition, an interesting sexually dimorphic expression of Smdnd during gonadal development was observed by real-time PCR. Female turbot showed greater (P < 0.05) Smdnd expression than male before sex maturation. This difference reduced gradually due to the upregulation of Smdnd in the male during the period corresponding to spermatogonia proliferation and meiosis. These results indicate that Smdnd can be used as a germ cell marker in turbot. In addition, the temporal and sex differences in Smdnd expression indicate that this gene may play different roles in gonadal development in both sexes. (C) 2013 Elsevier Inc. All rights reserved. |
| 英文摘要 | Germ cells are indispensable for gonadal development and fertility. However, the physiological mechanisms regulating germ cell development in marine fish are poorly understood due to a lack of germ cell markers. The dead end (dnd) gene is a vertebrate-specific component of germplasm crucial for primordial germ cells (PGCs) migration and development in teleosts. In this study, we identified a dnd homologue (Smdnd) in turbot (Scophthalmus maximus) and investigated its expression pattern during embryogenesis and gonadal development. The deduced amino acid sequence of Smdnd shared several conserved motifs of Dnd homologues as well as high identity to other Dnd proteins. Phylogenetic analysis revealed that the SmDnd was closely related to its teleost counterparts. Reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization revealed that Smdnd transcripts could be exclusively detected in germ cells, including presumptive PGC and adult male and female germ cells. In addition, an interesting sexually dimorphic expression of Smdnd during gonadal development was observed by real-time PCR. Female turbot showed greater (P < 0.05) Smdnd expression than male before sex maturation. This difference reduced gradually due to the upregulation of Smdnd in the male during the period corresponding to spermatogonia proliferation and meiosis. These results indicate that Smdnd can be used as a germ cell marker in turbot. In addition, the temporal and sex differences in Smdnd expression indicate that this gene may play different roles in gonadal development in both sexes. (C) 2013 Elsevier Inc. All rights reserved. |
| 学科主题 | Reproductive Biology ; Veterinary Sciences |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Reproductive Biology ; Veterinary Sciences |
| 研究领域[WOS] | Reproductive Biology ; Veterinary Sciences |
| 关键词[WOS] | MESSENGER-RNA ; ZEBRAFISH MIR-430 ; CELL DEVELOPMENT ; MIGRATION ; FISH ; VASA |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.theriogenology.2013.06.016 |
| 语种 | 英语 |
| WOS记录号 | WOS:000323857200017 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16702]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, J |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 3.State Ocean Adm, Inst Oceanog 3, Xiamen, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, F.,Zhao, C. Y.,Xu, S. H.,et al. Germ line-specific and sexually dimorphic expression of a dead end gene homologue in turbot (Scophthalmus maximus)[J]. THERIOGENOLOGY,2013,80(6):665-672. |
| APA | Lin, F..,Zhao, C. Y..,Xu, S. H..,Ma, D. Y..,Xiao, Z. Z..,...&Li, J.(2013).Germ line-specific and sexually dimorphic expression of a dead end gene homologue in turbot (Scophthalmus maximus).THERIOGENOLOGY,80(6),665-672. |
| MLA | Lin, F.,et al."Germ line-specific and sexually dimorphic expression of a dead end gene homologue in turbot (Scophthalmus maximus)".THERIOGENOLOGY 80.6(2013):665-672. |
入库方式： OAI收割
来源：海洋研究所
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