Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development
文献类型：期刊论文
| 作者 | Chi, Liang1,2; Xu, Shihong1; Xiao, Zhizhong1; Lin, Fan1,2; Ma, Daoyuan1; Zhao, Chunyan1,2 ; Xiao, Yongshuang1; Liu, Qinghua1; Li, Jun1
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| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 165期号:1页码:58-65 |
| 关键词 | Turbot (Scophthalmus maximus) Pepsinogen A Pepsinogen C Functional differences Development |
| ISSN号 | 1096-4959 |
| 通讯作者 | Liu, QH |
| 中文摘要 | We characterized the expression patterns of pepsinogen A (tPGA) and pepsinogen C (tPGC) in turbot (Scophthalmus maximus). Quantitative expression analysis showed that tPGC was preferentially expressed in early developmental stages, and that the tPGA mRNA expression level was higher in adult fish. Full-length cDNA constructs of tPGA and tPGC were 1307 bp (from which 377 amino acids were deduced); and 1430 bp (from which 385 amino acids were deduced), respectively. The deduced proteins of tPGA and tPGC possessed signal peptides of 17 amino acids and 20 amino acids respectively. The initial transcripts of tPGA and tPGC were detected at 22 days post hatching (dph), well after the formation of gastric glands (16 dph). This suggested that the morphologic development of gastric glands was not synchronous with their functional development. In addition, tPGA and tPGC mRNAs were also expressed in muscle and ovary at much lower levels than in stomach and esophagus. The distribution of tPGA and tPGC in the turbot was investigated using in-situ hybridization, and tPGA and tPGC were first detected in the esophagus and cardiac region of the stomach, and then throughout the stomach. (C) 2013 Elsevier Inc. All rights reserved. |
| 英文摘要 | We characterized the expression patterns of pepsinogen A (tPGA) and pepsinogen C (tPGC) in turbot (Scophthalmus maximus). Quantitative expression analysis showed that tPGC was preferentially expressed in early developmental stages, and that the tPGA mRNA expression level was higher in adult fish. Full-length cDNA constructs of tPGA and tPGC were 1307 bp (from which 377 amino acids were deduced); and 1430 bp (from which 385 amino acids were deduced), respectively. The deduced proteins of tPGA and tPGC possessed signal peptides of 17 amino acids and 20 amino acids respectively. The initial transcripts of tPGA and tPGC were detected at 22 days post hatching (dph), well after the formation of gastric glands (16 dph). This suggested that the morphologic development of gastric glands was not synchronous with their functional development. In addition, tPGA and tPGC mRNAs were also expressed in muscle and ovary at much lower levels than in stomach and esophagus. The distribution of tPGA and tPGC in the turbot was investigated using in-situ hybridization, and tPGA and tPGC were first detected in the esophagus and cardiac region of the stomach, and then throughout the stomach. (C) 2013 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology ; Zoology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 关键词[WOS] | ORANGE-SPOTTED GROUPER ; PORGY PAGRUS-PAGRUS ; DIGESTIVE ENZYMES ; EPINEPHELUS-COIOIDES ; GASTRIC-MUCOSA ; ANTARCTIC FISH ; EVOLUTION ; ONTOGENY ; IDENTIFICATION ; FUNCTIONALITY |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.cbpb.2013.03.002 |
| 语种 | 英语 |
| WOS记录号 | WOS:000318464900008 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16712]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chi, Liang,Xu, Shihong,Xiao, Zhizhong,et al. Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2013,165(1):58-65. |
| APA | Chi, Liang.,Xu, Shihong.,Xiao, Zhizhong.,Lin, Fan.,Ma, Daoyuan.,...&Li, Jun.(2013).Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,165(1),58-65. |
| MLA | Chi, Liang,et al."Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 165.1(2013):58-65. |
入库方式： OAI收割
来源：海洋研究所
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