Peptidomic analysis of Chinese shrimp (Fenneropenaeus chinensis) hemolymph by magnetic bead-based MALDI-TOF MS
文献类型：期刊论文
| 作者 | Wang Baojie1,2; Liu Mei1; Jiang Keyong1; Zhang Guofan1; Wang Lei1; Wang, L
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| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 出版日期 | 2013-03-01 |
| 卷号 | 31期号:2页码:407-415 |
| 关键词 | Fenneropenaeus chinensis hemolymph peptidome MALDI-TOF MS WCX-magnetic bead immune-challenged |
| ISSN号 | 0254-4059 |
| 通讯作者 | Wang, L |
| 中文摘要 | Peptides in shrimp hemolymph play an important role in the innate immune response. Analysis of hemolymph will help to detect and identify potential novel biomarkers of microbial infection. We used magnetic bead-based purification (ClinProt system) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) to characterize shrimp hemolymph peptides. Shrimp serum and plasma were used as the source of samples for comparative analysis, and it was found that serum was more suitable for shrimp hemolymph peptidomic analysis. To screen potential specific biomarkers in serum of immune-challenged shrimps, we applied magnetic bead-based MALDI-TOF MS to serum samples from 10 immune-challenged and 10 healthy shrimps. The spectra were analyzed using FlexAnalysis 3.0 and ClinProTools 2.1 software. Thirteen peptide peaks significantly different between the two groups were selected as candidate biomarkers of lipopolysaccharide (LPS)-infection. The diagnostic model established by genetic algorithm using five of these peaks was able to discriminate LPS-challenged shrimps from healthy control shrimps with a sensitivity of 90% and a specificity of 100%. Our approach in MALDITOF MS-based peptidomics is a powerful tool for screening bioactive peptides or biomarkers derived from hemolymph, and will help to enable a better understanding of the innate immune response of shrimps. |
| 英文摘要 | Peptides in shrimp hemolymph play an important role in the innate immune response. Analysis of hemolymph will help to detect and identify potential novel biomarkers of microbial infection. We used magnetic bead-based purification (ClinProt system) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) to characterize shrimp hemolymph peptides. Shrimp serum and plasma were used as the source of samples for comparative analysis, and it was found that serum was more suitable for shrimp hemolymph peptidomic analysis. To screen potential specific biomarkers in serum of immune-challenged shrimps, we applied magnetic bead-based MALDI-TOF MS to serum samples from 10 immune-challenged and 10 healthy shrimps. The spectra were analyzed using FlexAnalysis 3.0 and ClinProTools 2.1 software. Thirteen peptide peaks significantly different between the two groups were selected as candidate biomarkers of lipopolysaccharide (LPS)-infection. The diagnostic model established by genetic algorithm using five of these peaks was able to discriminate LPS-challenged shrimps from healthy control shrimps with a sensitivity of 90% and a specificity of 100%. Our approach in MALDITOF MS-based peptidomics is a powerful tool for screening bioactive peptides or biomarkers derived from hemolymph, and will help to enable a better understanding of the innate immune response of shrimps. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 学科主题 | Marine & Freshwater Biology ; Oceanography |
| 类目[WOS] | Limnology ; Oceanography |
| 研究领域[WOS] | Marine & Freshwater Biology ; Oceanography |
| 关键词[WOS] | FLIGHT MASS-SPECTROMETRY ; MOLECULAR-CLONING ; ANTIMICROBIAL PEPTIDE ; LITOPENAEUS-VANNAMEI ; PROTEOMIC ANALYSIS ; PENAEUS-MONODON ; IMMUNE-RESPONSE ; NATURAL LECTIN ; PURIFICATION ; EXPRESSION |
| 收录类别 | SCI |
| 原文出处 | 10.1007/s00343-013-2076-4 |
| 语种 | 英语 |
| WOS记录号 | WOS:000316018300017 |
| 公开日期 | 2014-07-17 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/16713]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 通讯作者 | Wang, L |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanl, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang Baojie,Liu Mei,Jiang Keyong,et al. Peptidomic analysis of Chinese shrimp (Fenneropenaeus chinensis) hemolymph by magnetic bead-based MALDI-TOF MS[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(2):407-415. |
| APA | Wang Baojie,Liu Mei,Jiang Keyong,Zhang Guofan,Wang Lei,&Wang, L.(2013).Peptidomic analysis of Chinese shrimp (Fenneropenaeus chinensis) hemolymph by magnetic bead-based MALDI-TOF MS.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(2),407-415. |
| MLA | Wang Baojie,et al."Peptidomic analysis of Chinese shrimp (Fenneropenaeus chinensis) hemolymph by magnetic bead-based MALDI-TOF MS".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.2(2013):407-415. |
入库方式： OAI收割
来源：海洋研究所
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